Strain Fitness in Escherichia coli BL21 around ECD_01426

Experiment: Bas18

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01425 and ECD_01426 overlap by 4 nucleotidesECD_01426 and ECD_01427 are separated by 81 nucleotides ECD_01425: ECD_01425 - nitrate reductase 2 (NRZ), beta subunit, at 1,492,990 to 1,494,534 _01425 ECD_01426: ECD_01426 - nitrate reductase 2 (NRZ), alpha subunit, at 1,494,531 to 1,498,271 _01426 ECD_01427: ECD_01427 - nitrate/nitrite transporter, at 1,498,353 to 1,499,741 _01427 Position (kb) 1494 1495 1496 1497 1498 1499Strain fitness (log2 ratio) -2 -1 0 1 2at 1493.547 kb on - strand, within ECD_01425at 1494.178 kb on - strand, within ECD_01425at 1494.787 kb on + strandat 1494.787 kb on + strandat 1494.971 kb on - strand, within ECD_01426at 1495.138 kb on + strand, within ECD_01426at 1495.208 kb on - strand, within ECD_01426at 1495.589 kb on + strand, within ECD_01426at 1496.095 kb on + strand, within ECD_01426at 1496.543 kb on - strand, within ECD_01426at 1497.224 kb on + strand, within ECD_01426at 1497.827 kb on - strand, within ECD_01426at 1498.109 kb on + strandat 1498.191 kb on - strandat 1498.335 kb on - strandat 1498.647 kb on - strand, within ECD_01427at 1498.818 kb on - strand, within ECD_01427at 1499.221 kb on - strand, within ECD_01427

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas18
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1,493,547 - ECD_01425 0.36 +0.4
1,494,178 - ECD_01425 0.77 +0.0
1,494,787 + -0.9
1,494,787 + -0.0
1,494,971 - ECD_01426 0.12 +0.5
1,495,138 + ECD_01426 0.16 +0.2
1,495,208 - ECD_01426 0.18 -1.1
1,495,589 + ECD_01426 0.28 -1.8
1,496,095 + ECD_01426 0.42 +1.8
1,496,543 - ECD_01426 0.54 -2.0
1,497,224 + ECD_01426 0.72 -1.1
1,497,827 - ECD_01426 0.88 -0.7
1,498,109 + +1.0
1,498,191 - -0.6
1,498,335 - -1.0
1,498,647 - ECD_01427 0.21 +0.2
1,498,818 - ECD_01427 0.33 -0.1
1,499,221 - ECD_01427 0.62 -1.2

Or see this region's nucleotide sequence