Strain Fitness in Escherichia coli BL21 around ECD_00194

Experiment: Bas18

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00193 and ECD_00194 are separated by 110 nucleotidesECD_00194 and ECD_00195 overlap by 4 nucleotidesECD_00195 and ECD_00196 are separated by 117 nucleotides ECD_00193: ECD_00193 - prolyl-tRNA synthetase, at 219,899 to 221,617 _00193 ECD_00194: ECD_00194 - tRNA-Thr(GGU) m(6)t(6)A37 methyltransferase, SAM-dependent, at 221,728 to 222,435 _00194 ECD_00195: ECD_00195 - putative outer membrane protein, at 222,432 to 222,836 _00195 ECD_00196: ECD_00196 - DL-methionine transporter subunit, at 222,954 to 223,769 _00196 Position (kb) 221 222 223Strain fitness (log2 ratio) -2 -1 0 1 2 3at 221.982 kb on - strand, within ECD_00194at 222.023 kb on + strand, within ECD_00194at 222.023 kb on + strand, within ECD_00194at 222.024 kb on - strand, within ECD_00194at 222.132 kb on + strand, within ECD_00194at 222.133 kb on - strand, within ECD_00194at 222.133 kb on - strand, within ECD_00194at 222.311 kb on + strand, within ECD_00194at 222.409 kb on + strandat 222.464 kb on + strandat 222.468 kb on + strandat 222.469 kb on - strandat 222.469 kb on - strandat 222.469 kb on - strandat 222.469 kb on - strandat 222.515 kb on + strand, within ECD_00195at 222.569 kb on + strand, within ECD_00195at 222.681 kb on - strand, within ECD_00195at 222.683 kb on - strand, within ECD_00195at 222.810 kb on + strandat 222.811 kb on - strandat 222.837 kb on - strandat 222.837 kb on - strandat 222.852 kb on + strandat 222.853 kb on - strandat 222.929 kb on - strandat 222.929 kb on - strandat 223.022 kb on - strandat 223.118 kb on + strand, within ECD_00196at 223.156 kb on + strand, within ECD_00196at 223.156 kb on + strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.157 kb on - strand, within ECD_00196at 223.349 kb on + strand, within ECD_00196

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas18
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221,982 - ECD_00194 0.36 -0.0
222,023 + ECD_00194 0.42 -0.6
222,023 + ECD_00194 0.42 +0.1
222,024 - ECD_00194 0.42 -1.0
222,132 + ECD_00194 0.57 -0.1
222,133 - ECD_00194 0.57 +0.2
222,133 - ECD_00194 0.57 -0.4
222,311 + ECD_00194 0.82 -1.8
222,409 + +0.4
222,464 + -0.1
222,468 + -1.5
222,469 - -0.4
222,469 - +0.1
222,469 - -0.5
222,469 - -0.0
222,515 + ECD_00195 0.20 +0.1
222,569 + ECD_00195 0.34 +1.0
222,681 - ECD_00195 0.61 -0.8
222,683 - ECD_00195 0.62 -0.3
222,810 + -0.1
222,811 - +0.4
222,837 - -0.1
222,837 - -2.0
222,852 + +0.2
222,853 - -0.7
222,929 - -0.3
222,929 - -0.1
223,022 - +1.9
223,118 + ECD_00196 0.20 +1.6
223,156 + ECD_00196 0.25 +1.8
223,156 + ECD_00196 0.25 +1.2
223,157 - ECD_00196 0.25 +1.7
223,157 - ECD_00196 0.25 +2.3
223,157 - ECD_00196 0.25 +1.4
223,157 - ECD_00196 0.25 +2.8
223,349 + ECD_00196 0.48 +2.6

Or see this region's nucleotide sequence