Strain Fitness in Pseudomonas simiae WCS417 around PS417_19560

Experiment: Growth on radish seeds for 72 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_19550 and PS417_19555 are separated by 302 nucleotidesPS417_19555 and PS417_19560 are separated by 42 nucleotidesPS417_19560 and PS417_19565 are separated by 94 nucleotidesPS417_19565 and PS417_19570 are separated by 153 nucleotides PS417_19550: PS417_19550 - thioesterase, at 4,252,436 to 4,253,188 _19550 PS417_19555: PS417_19555 - RNA polymerase sigma70, at 4,253,491 to 4,254,042 _19555 PS417_19560: PS417_19560 - amino acid ABC transporter substrate-binding protein, at 4,254,085 to 4,254,873 _19560 PS417_19565: PS417_19565 - TetR family transcriptional regulator, at 4,254,968 to 4,255,591 _19565 PS417_19570: PS417_19570 - terpene utilization protein AtuA, at 4,255,745 to 4,257,523 _19570 Position (kb) 4254 4255Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 4253.219 kb on + strandat 4253.219 kb on + strandat 4253.219 kb on + strandat 4253.220 kb on - strandat 4253.220 kb on - strandat 4253.220 kb on - strandat 4253.220 kb on - strandat 4253.220 kb on - strandat 4253.220 kb on - strandat 4253.220 kb on - strandat 4253.319 kb on - strandat 4253.357 kb on + strandat 4253.357 kb on + strandat 4253.435 kb on - strandat 4253.435 kb on - strandat 4253.504 kb on + strandat 4253.504 kb on + strandat 4253.505 kb on - strandat 4253.583 kb on + strand, within PS417_19555at 4253.584 kb on - strand, within PS417_19555at 4253.616 kb on - strand, within PS417_19555at 4253.616 kb on - strand, within PS417_19555at 4253.686 kb on + strand, within PS417_19555at 4253.686 kb on + strand, within PS417_19555at 4253.896 kb on + strand, within PS417_19555at 4254.022 kb on - strandat 4254.033 kb on + strandat 4254.033 kb on + strandat 4254.053 kb on + strandat 4254.054 kb on - strandat 4254.054 kb on - strandat 4254.113 kb on + strandat 4254.224 kb on + strand, within PS417_19560at 4254.224 kb on + strand, within PS417_19560at 4254.224 kb on + strand, within PS417_19560at 4254.225 kb on - strand, within PS417_19560at 4254.225 kb on - strand, within PS417_19560at 4254.225 kb on - strand, within PS417_19560at 4254.285 kb on + strand, within PS417_19560at 4254.285 kb on + strand, within PS417_19560at 4254.299 kb on + strand, within PS417_19560at 4254.300 kb on - strand, within PS417_19560at 4254.368 kb on + strand, within PS417_19560at 4254.368 kb on + strand, within PS417_19560at 4254.369 kb on - strand, within PS417_19560at 4254.401 kb on + strand, within PS417_19560at 4254.401 kb on + strand, within PS417_19560at 4254.401 kb on + strand, within PS417_19560at 4254.402 kb on - strand, within PS417_19560at 4254.402 kb on - strand, within PS417_19560at 4254.664 kb on + strand, within PS417_19560at 4254.755 kb on + strand, within PS417_19560at 4254.954 kb on + strandat 4255.095 kb on - strandat 4255.110 kb on + strand, within PS417_19565at 4255.136 kb on + strand, within PS417_19565at 4255.137 kb on - strand, within PS417_19565at 4255.159 kb on - strand, within PS417_19565at 4255.347 kb on + strand, within PS417_19565at 4255.348 kb on - strand, within PS417_19565at 4255.633 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth on radish seeds for 72 hours
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4,253,219 + +0.0
4,253,219 + +0.4
4,253,219 + -2.2
4,253,220 - +0.6
4,253,220 - +1.8
4,253,220 - +0.5
4,253,220 - -0.3
4,253,220 - -0.2
4,253,220 - +2.8
4,253,220 - -1.5
4,253,319 - +0.0
4,253,357 + +0.3
4,253,357 + -0.0
4,253,435 - +1.0
4,253,435 - -1.7
4,253,504 + +0.2
4,253,504 + -1.5
4,253,505 - +0.1
4,253,583 + PS417_19555 0.17 +0.8
4,253,584 - PS417_19555 0.17 +1.4
4,253,616 - PS417_19555 0.23 +0.4
4,253,616 - PS417_19555 0.23 +0.3
4,253,686 + PS417_19555 0.35 +1.6
4,253,686 + PS417_19555 0.35 -1.5
4,253,896 + PS417_19555 0.73 +2.1
4,254,022 - +0.2
4,254,033 + -0.7
4,254,033 + +0.8
4,254,053 + -0.7
4,254,054 - +0.5
4,254,054 - -1.3
4,254,113 + +0.7
4,254,224 + PS417_19560 0.18 +1.0
4,254,224 + PS417_19560 0.18 +2.7
4,254,224 + PS417_19560 0.18 +3.0
4,254,225 - PS417_19560 0.18 -0.2
4,254,225 - PS417_19560 0.18 +5.0
4,254,225 - PS417_19560 0.18 +1.6
4,254,285 + PS417_19560 0.25 +1.8
4,254,285 + PS417_19560 0.25 +0.2
4,254,299 + PS417_19560 0.27 +1.3
4,254,300 - PS417_19560 0.27 +1.5
4,254,368 + PS417_19560 0.36 +1.6
4,254,368 + PS417_19560 0.36 +1.7
4,254,369 - PS417_19560 0.36 -0.2
4,254,401 + PS417_19560 0.40 +1.4
4,254,401 + PS417_19560 0.40 +1.0
4,254,401 + PS417_19560 0.40 +2.9
4,254,402 - PS417_19560 0.40 +3.1
4,254,402 - PS417_19560 0.40 -0.2
4,254,664 + PS417_19560 0.73 +1.8
4,254,755 + PS417_19560 0.85 +4.2
4,254,954 + -0.4
4,255,095 - -0.2
4,255,110 + PS417_19565 0.23 -2.6
4,255,136 + PS417_19565 0.27 -2.0
4,255,137 - PS417_19565 0.27 -0.1
4,255,159 - PS417_19565 0.31 -1.0
4,255,347 + PS417_19565 0.61 -1.1
4,255,348 - PS417_19565 0.61 -0.6
4,255,633 + +0.8

Or see this region's nucleotide sequence