Experiment: Growth on radish seeds for 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_12230 and PS417_12235 are separated by 64 nucleotides PS417_12235 and PS417_12240 are separated by 127 nucleotides PS417_12240 and PS417_12245 are separated by 20 nucleotides PS417_12245 and PS417_12250 overlap by 11 nucleotides
PS417_12230: PS417_12230 - ribonuclease H, at 2,658,536 to 2,658,988
_12230
PS417_12235: PS417_12235 - DNA polymerase III subunit epsilon, at 2,659,053 to 2,659,799
_12235
PS417_12240: PS417_12240 - lysine decarboxylase, at 2,659,927 to 2,662,182
_12240
PS417_12245: PS417_12245 - GNAT family acetyltransferase, at 2,662,203 to 2,662,757
_12245
PS417_12250: PS417_12250 - LysR family transcriptional regulator, at 2,662,747 to 2,663,667
_12250
Position (kb)
2659
2660
2661
2662
2663 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2659.004 kb on + strand at 2659.007 kb on + strand at 2659.833 kb on + strand at 2659.834 kb on - strand at 2659.889 kb on + strand at 2659.916 kb on + strand at 2660.142 kb on + strand at 2660.143 kb on - strand at 2660.233 kb on - strand, within PS417_12240 at 2660.354 kb on - strand, within PS417_12240 at 2660.354 kb on - strand, within PS417_12240 at 2660.416 kb on + strand, within PS417_12240 at 2660.416 kb on + strand, within PS417_12240 at 2660.764 kb on + strand, within PS417_12240 at 2660.764 kb on + strand, within PS417_12240 at 2660.765 kb on - strand, within PS417_12240 at 2660.765 kb on - strand, within PS417_12240 at 2660.765 kb on - strand, within PS417_12240 at 2660.917 kb on + strand, within PS417_12240 at 2660.918 kb on - strand, within PS417_12240 at 2660.918 kb on - strand, within PS417_12240 at 2660.933 kb on - strand, within PS417_12240 at 2660.967 kb on + strand, within PS417_12240 at 2661.204 kb on + strand, within PS417_12240 at 2661.492 kb on - strand, within PS417_12240 at 2661.628 kb on + strand, within PS417_12240 at 2661.641 kb on + strand, within PS417_12240 at 2661.641 kb on + strand, within PS417_12240 at 2661.826 kb on + strand, within PS417_12240 at 2661.866 kb on - strand, within PS417_12240 at 2661.908 kb on - strand, within PS417_12240 at 2662.157 kb on - strand at 2662.157 kb on - strand at 2662.190 kb on + strand at 2662.190 kb on + strand at 2662.190 kb on + strand at 2662.216 kb on - strand at 2662.261 kb on - strand, within PS417_12245 at 2662.351 kb on + strand, within PS417_12245 at 2662.464 kb on - strand, within PS417_12245 at 2662.464 kb on - strand, within PS417_12245 at 2662.647 kb on + strand, within PS417_12245 at 2662.662 kb on + strand at 2662.755 kb on + strand at 2662.868 kb on + strand, within PS417_12250 at 2662.997 kb on + strand, within PS417_12250 at 2662.998 kb on - strand, within PS417_12250 at 2663.129 kb on + strand, within PS417_12250 at 2663.130 kb on - strand, within PS417_12250 at 2663.130 kb on - strand, within PS417_12250
Per-strain Table
Position Strand Gene LocusTag Fraction Growth on radish seeds for 72 hours remove 2,659,004 + -0.2 2,659,007 + +1.0 2,659,833 + -0.0 2,659,834 - +0.5 2,659,889 + -1.4 2,659,916 + +1.5 2,660,142 + +2.1 2,660,143 - -1.1 2,660,233 - PS417_12240 0.14 -1.1 2,660,354 - PS417_12240 0.19 -2.3 2,660,354 - PS417_12240 0.19 -0.5 2,660,416 + PS417_12240 0.22 -1.1 2,660,416 + PS417_12240 0.22 -1.8 2,660,764 + PS417_12240 0.37 -2.6 2,660,764 + PS417_12240 0.37 -1.1 2,660,765 - PS417_12240 0.37 -0.0 2,660,765 - PS417_12240 0.37 -1.3 2,660,765 - PS417_12240 0.37 +0.6 2,660,917 + PS417_12240 0.44 -0.5 2,660,918 - PS417_12240 0.44 -1.1 2,660,918 - PS417_12240 0.44 +0.2 2,660,933 - PS417_12240 0.45 -0.1 2,660,967 + PS417_12240 0.46 +1.5 2,661,204 + PS417_12240 0.57 +0.6 2,661,492 - PS417_12240 0.69 -0.1 2,661,628 + PS417_12240 0.75 -0.5 2,661,641 + PS417_12240 0.76 -0.1 2,661,641 + PS417_12240 0.76 -0.5 2,661,826 + PS417_12240 0.84 +0.5 2,661,866 - PS417_12240 0.86 -0.6 2,661,908 - PS417_12240 0.88 +0.2 2,662,157 - -0.5 2,662,157 - +0.7 2,662,190 + -1.1 2,662,190 + +0.9 2,662,190 + +0.5 2,662,216 - +0.1 2,662,261 - PS417_12245 0.10 +0.2 2,662,351 + PS417_12245 0.27 -0.5 2,662,464 - PS417_12245 0.47 +0.5 2,662,464 - PS417_12245 0.47 -0.1 2,662,647 + PS417_12245 0.80 +1.1 2,662,662 + +1.5 2,662,755 + +0.5 2,662,868 + PS417_12250 0.13 +0.2 2,662,997 + PS417_12250 0.27 -1.5 2,662,998 - PS417_12250 0.27 -1.5 2,663,129 + PS417_12250 0.41 -1.5 2,663,130 - PS417_12250 0.42 -1.1 2,663,130 - PS417_12250 0.42 -0.3
Or see this region's nucleotide sequence