Experiment: LB Anaerobic with Hygromycin B 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt betA and betB are separated by 13 nucleotides betB and betI are separated by 13 nucleotides betI and betT are separated by 128 nucleotides
b0311: betA - choline dehydrogenase (NCBI), at 324,801 to 326,471
betA
b0312: betB - betaine aldehyde dehydrogenase, NAD-dependent (NCBI), at 326,485 to 327,957
betB
b0313: betI - probably transcriptional repressor of bet genes (VIMSS), at 327,971 to 328,558
betI
b0314: betT - choline transporter of high affinity (NCBI), at 328,687 to 330,720
betT
Position (kb) 326
327
328 Strain fitness (log2 ratio) -2
-1
0
1
2 at 325.506 kb on + strand, within betA at 325.513 kb on - strand, within betA at 325.513 kb on - strand, within betA at 325.513 kb on - strand, within betA at 325.520 kb on + strand, within betA at 325.520 kb on + strand, within betA at 325.525 kb on + strand, within betA at 325.525 kb on - strand, within betA at 325.533 kb on - strand, within betA at 325.533 kb on - strand, within betA at 325.551 kb on - strand, within betA at 325.707 kb on - strand, within betA at 325.714 kb on + strand, within betA at 325.714 kb on + strand, within betA at 325.752 kb on - strand, within betA at 325.835 kb on - strand, within betA at 325.835 kb on - strand, within betA at 325.858 kb on + strand, within betA at 325.858 kb on + strand, within betA at 325.873 kb on + strand, within betA at 325.873 kb on + strand, within betA at 325.890 kb on - strand, within betA at 325.914 kb on - strand, within betA at 325.968 kb on - strand, within betA at 325.968 kb on - strand, within betA at 326.137 kb on - strand, within betA at 326.214 kb on + strand, within betA at 326.384 kb on - strand at 326.384 kb on - strand at 326.435 kb on - strand at 326.524 kb on - strand at 326.537 kb on + strand at 326.691 kb on - strand, within betB at 326.698 kb on + strand, within betB at 326.698 kb on + strand, within betB at 326.722 kb on - strand, within betB at 326.722 kb on - strand, within betB at 326.782 kb on - strand, within betB at 326.782 kb on - strand, within betB at 326.816 kb on - strand, within betB at 326.816 kb on - strand, within betB at 326.871 kb on - strand, within betB at 326.912 kb on - strand, within betB at 326.936 kb on + strand, within betB at 326.944 kb on - strand, within betB at 327.090 kb on + strand, within betB at 327.097 kb on + strand, within betB at 327.097 kb on + strand, within betB at 327.101 kb on - strand, within betB at 327.413 kb on - strand, within betB at 327.422 kb on - strand, within betB at 327.447 kb on + strand, within betB at 327.480 kb on + strand, within betB at 327.480 kb on + strand, within betB at 327.480 kb on + strand, within betB at 327.544 kb on + strand, within betB at 327.584 kb on + strand, within betB at 327.606 kb on - strand, within betB at 327.723 kb on + strand, within betB at 327.723 kb on + strand, within betB at 327.870 kb on - strand at 327.870 kb on - strand at 327.910 kb on + strand at 327.910 kb on + strand at 328.001 kb on - strand at 328.009 kb on - strand at 328.054 kb on - strand, within betI at 328.104 kb on + strand, within betI at 328.104 kb on + strand, within betI at 328.188 kb on + strand, within betI at 328.213 kb on - strand, within betI at 328.213 kb on - strand, within betI at 328.220 kb on - strand, within betI at 328.303 kb on + strand, within betI at 328.303 kb on + strand, within betI at 328.360 kb on + strand, within betI at 328.475 kb on + strand, within betI at 328.475 kb on + strand, within betI at 328.483 kb on - strand, within betI at 328.484 kb on - strand, within betI at 328.526 kb on + strand at 328.526 kb on + strand at 328.566 kb on + strand at 328.628 kb on - strand at 328.628 kb on - strand at 328.758 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Hygromycin B 0.000125 mM remove 325,506 + betA b0311 0.42 -0.2 325,513 - betA b0311 0.43 +0.4 325,513 - betA b0311 0.43 +0.4 325,513 - betA b0311 0.43 +1.0 325,520 + betA b0311 0.43 -0.4 325,520 + betA b0311 0.43 +0.5 325,525 + betA b0311 0.43 +0.4 325,525 - betA b0311 0.43 -0.5 325,533 - betA b0311 0.44 +0.5 325,533 - betA b0311 0.44 +0.6 325,551 - betA b0311 0.45 +0.1 325,707 - betA b0311 0.54 -2.6 325,714 + betA b0311 0.55 -0.8 325,714 + betA b0311 0.55 -0.4 325,752 - betA b0311 0.57 +0.3 325,835 - betA b0311 0.62 +1.0 325,835 - betA b0311 0.62 +0.4 325,858 + betA b0311 0.63 +0.2 325,858 + betA b0311 0.63 +1.3 325,873 + betA b0311 0.64 +1.1 325,873 + betA b0311 0.64 +0.5 325,890 - betA b0311 0.65 -0.9 325,914 - betA b0311 0.67 -0.3 325,968 - betA b0311 0.70 +0.2 325,968 - betA b0311 0.70 +0.3 326,137 - betA b0311 0.80 +0.9 326,214 + betA b0311 0.85 +0.2 326,384 - -0.2 326,384 - -0.3 326,435 - +0.2 326,524 - -0.2 326,537 + +1.8 326,691 - betB b0312 0.14 -0.7 326,698 + betB b0312 0.14 -0.6 326,698 + betB b0312 0.14 -0.6 326,722 - betB b0312 0.16 -0.3 326,722 - betB b0312 0.16 -0.1 326,782 - betB b0312 0.20 -1.3 326,782 - betB b0312 0.20 +0.8 326,816 - betB b0312 0.22 -1.0 326,816 - betB b0312 0.22 +0.6 326,871 - betB b0312 0.26 -0.3 326,912 - betB b0312 0.29 -0.6 326,936 + betB b0312 0.31 +0.7 326,944 - betB b0312 0.31 -0.5 327,090 + betB b0312 0.41 -0.6 327,097 + betB b0312 0.42 +0.8 327,097 + betB b0312 0.42 -0.8 327,101 - betB b0312 0.42 +1.2 327,413 - betB b0312 0.63 -0.8 327,422 - betB b0312 0.64 +0.1 327,447 + betB b0312 0.65 +0.0 327,480 + betB b0312 0.68 -0.0 327,480 + betB b0312 0.68 -0.9 327,480 + betB b0312 0.68 +0.6 327,544 + betB b0312 0.72 +0.2 327,584 + betB b0312 0.75 -1.8 327,606 - betB b0312 0.76 -0.4 327,723 + betB b0312 0.84 -1.2 327,723 + betB b0312 0.84 -2.0 327,870 - +0.8 327,870 - +0.5 327,910 + +0.3 327,910 + +2.4 328,001 - -0.1 328,009 - +0.0 328,054 - betI b0313 0.14 -0.9 328,104 + betI b0313 0.23 +0.8 328,104 + betI b0313 0.23 +0.3 328,188 + betI b0313 0.37 +1.0 328,213 - betI b0313 0.41 -0.6 328,213 - betI b0313 0.41 -0.2 328,220 - betI b0313 0.42 -0.2 328,303 + betI b0313 0.56 +0.1 328,303 + betI b0313 0.56 +0.5 328,360 + betI b0313 0.66 +0.2 328,475 + betI b0313 0.86 -0.1 328,475 + betI b0313 0.86 +0.5 328,483 - betI b0313 0.87 -0.4 328,484 - betI b0313 0.87 +0.4 328,526 + +0.4 328,526 + +0.5 328,566 + +0.5 328,628 - -0.6 328,628 - +1.1 328,758 + -0.5
Or see this region's nucleotide sequence