Strain Fitness in Escherichia coli BW25113 around b1890

Experiment: LB Anaerobic with Hygromycin B 0.00025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcheA and motB are separated by 4 nucleotidesmotB and motA overlap by 4 nucleotidesmotA and flhC are separated by 126 nucleotidesflhC and flhD are separated by 2 nucleotides b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348 cheA b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279 motB b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163 motA b1891: flhC - DNA-binding transcriptional dual regulator with FlhD (NCBI), at 1,975,290 to 1,975,868 flhC b1892: flhD - DNA-binding transcriptional dual regulator with FlhC (RefSeq), at 1,975,871 to 1,976,221 flhD Position (kb) 1974 1975 1976Strain fitness (log2 ratio) -1 0 1 2 3at 1973.279 kb on - strandat 1973.279 kb on - strandat 1973.443 kb on - strandat 1973.443 kb on - strandat 1973.577 kb on - strand, within motBat 1973.595 kb on - strand, within motBat 1973.697 kb on + strand, within motBat 1973.846 kb on - strand, within motBat 1973.850 kb on - strand, within motBat 1974.089 kb on - strand, within motBat 1974.089 kb on - strand, within motBat 1974.197 kb on + strandat 1974.226 kb on + strandat 1974.233 kb on + strandat 1974.233 kb on + strandat 1974.414 kb on + strand, within motAat 1974.854 kb on - strand, within motAat 1974.934 kb on + strand, within motAat 1974.934 kb on + strand, within motAat 1975.040 kb on + strand, within motAat 1975.092 kb on + strandat 1975.093 kb on - strandat 1975.173 kb on + strandat 1975.179 kb on + strandat 1975.179 kb on + strandat 1975.415 kb on - strand, within flhCat 1975.477 kb on + strand, within flhCat 1975.589 kb on + strand, within flhCat 1975.636 kb on + strand, within flhCat 1975.707 kb on + strand, within flhCat 1975.914 kb on - strand, within flhDat 1975.961 kb on - strand, within flhDat 1975.961 kb on - strand, within flhDat 1975.991 kb on + strand, within flhDat 1975.991 kb on - strand, within flhDat 1976.072 kb on + strand, within flhDat 1976.072 kb on + strand, within flhDat 1976.072 kb on + strand, within flhDat 1976.120 kb on + strand, within flhDat 1976.120 kb on + strand, within flhDat 1976.138 kb on + strand, within flhDat 1976.146 kb on - strand, within flhDat 1976.149 kb on - strand, within flhDat 1976.149 kb on - strand, within flhD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Hygromycin B 0.00025 mM
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1,973,279 - -0.4
1,973,279 - -0.0
1,973,443 - +3.0
1,973,443 - -0.9
1,973,577 - motB b1889 0.24 -0.1
1,973,595 - motB b1889 0.26 -0.2
1,973,697 + motB b1889 0.37 -0.1
1,973,846 - motB b1889 0.53 -0.5
1,973,850 - motB b1889 0.54 +0.7
1,974,089 - motB b1889 0.79 -0.0
1,974,089 - motB b1889 0.79 +0.7
1,974,197 + +0.1
1,974,226 + +0.5
1,974,233 + -1.1
1,974,233 + +1.2
1,974,414 + motA b1890 0.16 -1.3
1,974,854 - motA b1890 0.65 -1.1
1,974,934 + motA b1890 0.74 -0.3
1,974,934 + motA b1890 0.74 -0.4
1,975,040 + motA b1890 0.86 -1.1
1,975,092 + +0.1
1,975,093 - +0.2
1,975,173 + +0.4
1,975,179 + -0.5
1,975,179 + +1.3
1,975,415 - flhC b1891 0.22 -0.2
1,975,477 + flhC b1891 0.32 -0.1
1,975,589 + flhC b1891 0.52 +0.5
1,975,636 + flhC b1891 0.60 -0.5
1,975,707 + flhC b1891 0.72 -0.3
1,975,914 - flhD b1892 0.12 +0.8
1,975,961 - flhD b1892 0.26 +0.5
1,975,961 - flhD b1892 0.26 +0.0
1,975,991 + flhD b1892 0.34 -0.9
1,975,991 - flhD b1892 0.34 -0.2
1,976,072 + flhD b1892 0.57 -1.5
1,976,072 + flhD b1892 0.57 -0.0
1,976,072 + flhD b1892 0.57 +0.6
1,976,120 + flhD b1892 0.71 +0.1
1,976,120 + flhD b1892 0.71 +0.3
1,976,138 + flhD b1892 0.76 -1.1
1,976,146 - flhD b1892 0.78 +0.5
1,976,149 - flhD b1892 0.79 -1.1
1,976,149 - flhD b1892 0.79 -1.3

Or see this region's nucleotide sequence