Experiment: LB Anaerobic with Tetracycline 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybC and hybB overlap by 4 nucleotides hybB and hybA overlap by 11 nucleotides hybA and hybO are separated by 2 nucleotides
b2994: hybC - hydrogenase 2, large subunit (NCBI), at 3,139,308 to 3,141,011
hybC
b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186
hybB
b2996: hybA - hydrogenase 2 4Fe-4S ferredoxin-type component (NCBI), at 3,142,176 to 3,143,162
hybA
b2997: hybO - hydrogenase 2, small subunit (NCBI), at 3,143,165 to 3,144,283
hybO
Position (kb)
3141
3142
3143 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3140.013 kb on - strand, within hybC at 3140.013 kb on - strand, within hybC at 3140.129 kb on + strand, within hybC at 3140.159 kb on + strand, within hybC at 3140.167 kb on - strand, within hybC at 3140.167 kb on - strand, within hybC at 3140.227 kb on + strand at 3140.227 kb on + strand, within hybC at 3140.235 kb on + strand, within hybC at 3140.284 kb on + strand, within hybC at 3140.320 kb on - strand, within hybC at 3140.361 kb on + strand, within hybC at 3140.391 kb on - strand, within hybC at 3140.418 kb on + strand, within hybC at 3140.418 kb on + strand, within hybC at 3140.525 kb on - strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.591 kb on - strand, within hybC at 3140.591 kb on - strand, within hybC at 3140.641 kb on - strand, within hybC at 3140.657 kb on + strand, within hybC at 3140.657 kb on + strand, within hybC at 3140.823 kb on - strand, within hybC at 3140.838 kb on + strand, within hybC at 3140.838 kb on + strand, within hybC at 3140.852 kb on - strand at 3140.885 kb on + strand at 3141.123 kb on + strand at 3141.192 kb on - strand, within hybB at 3141.215 kb on + strand, within hybB at 3141.215 kb on + strand, within hybB at 3141.223 kb on - strand, within hybB at 3141.240 kb on - strand, within hybB at 3141.240 kb on - strand, within hybB at 3141.286 kb on - strand, within hybB at 3141.341 kb on - strand, within hybB at 3141.341 kb on - strand, within hybB at 3141.472 kb on + strand, within hybB at 3141.486 kb on + strand, within hybB at 3141.554 kb on - strand, within hybB at 3141.632 kb on + strand, within hybB at 3141.642 kb on + strand, within hybB at 3141.642 kb on + strand, within hybB at 3141.657 kb on - strand, within hybB at 3141.676 kb on - strand, within hybB at 3141.733 kb on - strand, within hybB at 3141.738 kb on - strand, within hybB at 3141.738 kb on - strand, within hybB at 3141.751 kb on - strand, within hybB at 3141.751 kb on - strand, within hybB at 3141.779 kb on - strand, within hybB at 3141.804 kb on - strand, within hybB at 3141.808 kb on + strand, within hybB at 3141.843 kb on + strand, within hybB at 3141.851 kb on - strand, within hybB at 3141.888 kb on + strand, within hybB at 3141.901 kb on - strand, within hybB at 3141.901 kb on - strand, within hybB at 3141.913 kb on - strand, within hybB at 3141.955 kb on - strand, within hybB at 3141.955 kb on - strand, within hybB at 3142.110 kb on - strand at 3142.110 kb on - strand at 3142.271 kb on - strand at 3142.284 kb on - strand, within hybA at 3142.284 kb on - strand, within hybA at 3142.284 kb on - strand, within hybA at 3142.286 kb on - strand, within hybA at 3142.286 kb on - strand, within hybA at 3142.368 kb on + strand, within hybA at 3142.410 kb on + strand, within hybA at 3142.446 kb on - strand, within hybA at 3142.585 kb on - strand, within hybA at 3142.585 kb on - strand, within hybA at 3142.587 kb on + strand, within hybA at 3142.600 kb on + strand, within hybA at 3142.676 kb on - strand, within hybA at 3142.719 kb on - strand, within hybA at 3142.719 kb on - strand, within hybA at 3142.865 kb on + strand, within hybA at 3142.877 kb on + strand, within hybA at 3142.885 kb on - strand, within hybA at 3142.995 kb on - strand, within hybA at 3142.995 kb on - strand, within hybA at 3143.008 kb on + strand, within hybA at 3143.108 kb on - strand at 3143.108 kb on - strand at 3143.127 kb on + strand at 3143.127 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Tetracycline 0.0000003125 mM remove 3,140,013 - hybC b2994 0.41 -2.4 3,140,013 - hybC b2994 0.41 +0.6 3,140,129 + hybC b2994 0.48 -0.7 3,140,159 + hybC b2994 0.50 -1.0 3,140,167 - hybC b2994 0.50 -1.0 3,140,167 - hybC b2994 0.50 +0.4 3,140,227 + -0.1 3,140,227 + hybC b2994 0.54 +0.6 3,140,235 + hybC b2994 0.54 -0.4 3,140,284 + hybC b2994 0.57 -0.7 3,140,320 - hybC b2994 0.59 -1.0 3,140,361 + hybC b2994 0.62 -0.4 3,140,391 - hybC b2994 0.64 -0.6 3,140,418 + hybC b2994 0.65 -0.3 3,140,418 + hybC b2994 0.65 +0.4 3,140,525 - hybC b2994 0.71 -0.4 3,140,583 + hybC b2994 0.75 -0.9 3,140,583 + hybC b2994 0.75 -0.4 3,140,583 + hybC b2994 0.75 -1.2 3,140,583 + hybC b2994 0.75 -1.1 3,140,591 - hybC b2994 0.75 +0.9 3,140,591 - hybC b2994 0.75 -0.3 3,140,641 - hybC b2994 0.78 -1.6 3,140,657 + hybC b2994 0.79 -1.0 3,140,657 + hybC b2994 0.79 -2.1 3,140,823 - hybC b2994 0.89 -2.5 3,140,838 + hybC b2994 0.90 -0.1 3,140,838 + hybC b2994 0.90 -0.9 3,140,852 - -0.7 3,140,885 + +0.1 3,141,123 + -0.4 3,141,192 - hybB b2995 0.16 +1.6 3,141,215 + hybB b2995 0.18 -0.3 3,141,215 + hybB b2995 0.18 -0.9 3,141,223 - hybB b2995 0.18 -1.0 3,141,240 - hybB b2995 0.20 -0.1 3,141,240 - hybB b2995 0.20 -1.1 3,141,286 - hybB b2995 0.24 -0.4 3,141,341 - hybB b2995 0.28 +0.2 3,141,341 - hybB b2995 0.28 -1.7 3,141,472 + hybB b2995 0.39 +0.3 3,141,486 + hybB b2995 0.41 -1.7 3,141,554 - hybB b2995 0.46 -1.3 3,141,632 + hybB b2995 0.53 +0.3 3,141,642 + hybB b2995 0.54 -1.0 3,141,642 + hybB b2995 0.54 -0.3 3,141,657 - hybB b2995 0.55 -1.9 3,141,676 - hybB b2995 0.57 -0.8 3,141,733 - hybB b2995 0.61 -0.8 3,141,738 - hybB b2995 0.62 -0.3 3,141,738 - hybB b2995 0.62 -1.4 3,141,751 - hybB b2995 0.63 +0.3 3,141,751 - hybB b2995 0.63 +0.0 3,141,779 - hybB b2995 0.65 -1.2 3,141,804 - hybB b2995 0.68 -1.4 3,141,808 + hybB b2995 0.68 -0.6 3,141,843 + hybB b2995 0.71 -0.2 3,141,851 - hybB b2995 0.72 -0.7 3,141,888 + hybB b2995 0.75 -0.8 3,141,901 - hybB b2995 0.76 +0.1 3,141,901 - hybB b2995 0.76 -0.5 3,141,913 - hybB b2995 0.77 -1.0 3,141,955 - hybB b2995 0.80 +0.5 3,141,955 - hybB b2995 0.80 -1.3 3,142,110 - -0.4 3,142,110 - -1.8 3,142,271 - -1.4 3,142,284 - hybA b2996 0.11 -0.9 3,142,284 - hybA b2996 0.11 -2.3 3,142,284 - hybA b2996 0.11 +0.4 3,142,286 - hybA b2996 0.11 -0.7 3,142,286 - hybA b2996 0.11 -0.1 3,142,368 + hybA b2996 0.19 -0.6 3,142,410 + hybA b2996 0.24 -0.3 3,142,446 - hybA b2996 0.27 -1.8 3,142,585 - hybA b2996 0.41 -0.9 3,142,585 - hybA b2996 0.41 -0.3 3,142,587 + hybA b2996 0.42 -0.9 3,142,600 + hybA b2996 0.43 -0.8 3,142,676 - hybA b2996 0.51 -0.2 3,142,719 - hybA b2996 0.55 -0.8 3,142,719 - hybA b2996 0.55 -0.3 3,142,865 + hybA b2996 0.70 +0.5 3,142,877 + hybA b2996 0.71 -0.6 3,142,885 - hybA b2996 0.72 -0.6 3,142,995 - hybA b2996 0.83 -0.2 3,142,995 - hybA b2996 0.83 +0.7 3,143,008 + hybA b2996 0.84 +0.3 3,143,108 - -0.1 3,143,108 - -2.1 3,143,127 + -3.0 3,143,127 + -0.7
Or see this region's nucleotide sequence