Experiment: LB Anaerobic with Tetracycline 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt norW and hypF are separated by 127 nucleotides hypF and hydN are separated by 152 nucleotides hydN and ascG are separated by 148 nucleotides
b2711: norW - nitric oxide reductase (NCBI), at 2,831,934 to 2,833,067
norW
b2712: hypF - carbamoyl phosphate phosphatase and maturation protein for [NiFe] hydrogenases (NCBI), at 2,833,195 to 2,835,447
hypF
b2713: hydN - formate dehydrogenase-H, [4Fe-4S] ferredoxin subunit (NCBI), at 2,835,600 to 2,836,127
hydN
b2714: ascG - DNA-binding transcriptional repressor (NCBI), at 2,836,276 to 2,837,286
ascG
Position (kb)
2833
2834
2835
2836 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2832.281 kb on + strand, within norW at 2832.281 kb on + strand, within norW at 2832.364 kb on + strand, within norW at 2832.384 kb on - strand, within norW at 2832.384 kb on - strand, within norW at 2832.526 kb on + strand, within norW at 2832.896 kb on - strand, within norW at 2832.956 kb on + strand at 2833.005 kb on + strand at 2833.005 kb on + strand at 2833.046 kb on + strand at 2833.054 kb on - strand at 2833.119 kb on + strand at 2833.119 kb on + strand at 2833.350 kb on + strand at 2833.350 kb on + strand at 2833.353 kb on - strand at 2833.371 kb on + strand at 2833.399 kb on - strand at 2833.399 kb on - strand at 2833.408 kb on + strand at 2833.423 kb on + strand, within hypF at 2833.423 kb on + strand, within hypF at 2833.581 kb on + strand, within hypF at 2833.699 kb on + strand, within hypF at 2833.734 kb on + strand, within hypF at 2833.934 kb on - strand, within hypF at 2833.962 kb on + strand, within hypF at 2833.962 kb on + strand, within hypF at 2833.980 kb on - strand, within hypF at 2834.076 kb on - strand, within hypF at 2834.208 kb on - strand, within hypF at 2834.211 kb on + strand, within hypF at 2834.211 kb on + strand, within hypF at 2834.358 kb on + strand, within hypF at 2834.547 kb on + strand, within hypF at 2834.574 kb on + strand, within hypF at 2834.574 kb on + strand, within hypF at 2834.576 kb on - strand, within hypF at 2834.576 kb on - strand, within hypF at 2834.689 kb on - strand, within hypF at 2834.832 kb on - strand, within hypF at 2834.862 kb on + strand, within hypF at 2834.878 kb on - strand, within hypF at 2834.951 kb on - strand, within hypF at 2834.951 kb on - strand, within hypF at 2834.955 kb on + strand, within hypF at 2834.972 kb on + strand, within hypF at 2835.007 kb on + strand, within hypF at 2835.007 kb on + strand, within hypF at 2835.051 kb on + strand, within hypF at 2835.051 kb on + strand, within hypF at 2835.172 kb on - strand, within hypF at 2835.172 kb on - strand, within hypF at 2835.235 kb on - strand at 2835.264 kb on - strand at 2835.306 kb on + strand at 2835.415 kb on + strand at 2835.522 kb on - strand at 2835.522 kb on - strand at 2835.729 kb on - strand, within hydN at 2835.731 kb on - strand, within hydN at 2835.812 kb on - strand, within hydN at 2835.849 kb on - strand, within hydN at 2835.849 kb on - strand, within hydN at 2835.902 kb on + strand, within hydN at 2835.933 kb on + strand, within hydN at 2835.982 kb on + strand, within hydN at 2836.033 kb on - strand, within hydN at 2836.033 kb on - strand, within hydN at 2836.043 kb on - strand, within hydN at 2836.043 kb on - strand, within hydN at 2836.078 kb on - strand at 2836.436 kb on + strand, within ascG at 2836.444 kb on - strand, within ascG at 2836.444 kb on - strand, within ascG at 2836.444 kb on - strand, within ascG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Tetracycline 0.0000003125 mM remove 2,832,281 + norW b2711 0.31 +0.4 2,832,281 + norW b2711 0.31 +0.3 2,832,364 + norW b2711 0.38 +0.4 2,832,384 - norW b2711 0.40 +1.1 2,832,384 - norW b2711 0.40 -0.2 2,832,526 + norW b2711 0.52 +0.7 2,832,896 - norW b2711 0.85 -0.1 2,832,956 + +0.4 2,833,005 + +0.1 2,833,005 + +1.0 2,833,046 + +0.1 2,833,054 - -0.0 2,833,119 + +0.1 2,833,119 + +1.3 2,833,350 + -0.1 2,833,350 + -0.3 2,833,353 - -0.8 2,833,371 + -1.6 2,833,399 - -0.5 2,833,399 - -1.1 2,833,408 + +1.6 2,833,423 + hypF b2712 0.10 -0.5 2,833,423 + hypF b2712 0.10 -0.4 2,833,581 + hypF b2712 0.17 -2.0 2,833,699 + hypF b2712 0.22 +0.6 2,833,734 + hypF b2712 0.24 -1.2 2,833,934 - hypF b2712 0.33 -0.6 2,833,962 + hypF b2712 0.34 -0.4 2,833,962 + hypF b2712 0.34 -0.8 2,833,980 - hypF b2712 0.35 -0.1 2,834,076 - hypF b2712 0.39 -1.0 2,834,208 - hypF b2712 0.45 -1.5 2,834,211 + hypF b2712 0.45 -0.6 2,834,211 + hypF b2712 0.45 -1.0 2,834,358 + hypF b2712 0.52 +0.3 2,834,547 + hypF b2712 0.60 -0.8 2,834,574 + hypF b2712 0.61 -1.5 2,834,574 + hypF b2712 0.61 -1.2 2,834,576 - hypF b2712 0.61 +0.2 2,834,576 - hypF b2712 0.61 -0.5 2,834,689 - hypF b2712 0.66 +0.8 2,834,832 - hypF b2712 0.73 -0.8 2,834,862 + hypF b2712 0.74 -1.1 2,834,878 - hypF b2712 0.75 -0.4 2,834,951 - hypF b2712 0.78 -0.6 2,834,951 - hypF b2712 0.78 -0.4 2,834,955 + hypF b2712 0.78 -1.0 2,834,972 + hypF b2712 0.79 -0.1 2,835,007 + hypF b2712 0.80 -0.6 2,835,007 + hypF b2712 0.80 -1.2 2,835,051 + hypF b2712 0.82 -0.9 2,835,051 + hypF b2712 0.82 -2.8 2,835,172 - hypF b2712 0.88 +1.8 2,835,172 - hypF b2712 0.88 -0.2 2,835,235 - -0.3 2,835,264 - -0.8 2,835,306 + -0.6 2,835,415 + -1.1 2,835,522 - -1.5 2,835,522 - -0.2 2,835,729 - hydN b2713 0.24 -0.5 2,835,731 - hydN b2713 0.25 -0.1 2,835,812 - hydN b2713 0.40 +0.4 2,835,849 - hydN b2713 0.47 -1.0 2,835,849 - hydN b2713 0.47 -0.9 2,835,902 + hydN b2713 0.57 +0.2 2,835,933 + hydN b2713 0.63 +0.1 2,835,982 + hydN b2713 0.72 +0.4 2,836,033 - hydN b2713 0.82 +0.2 2,836,033 - hydN b2713 0.82 +0.2 2,836,043 - hydN b2713 0.84 +0.2 2,836,043 - hydN b2713 0.84 -0.1 2,836,078 - +0.2 2,836,436 + ascG b2714 0.16 -0.1 2,836,444 - ascG b2714 0.17 +0.3 2,836,444 - ascG b2714 0.17 -0.2 2,836,444 - ascG b2714 0.17 -0.1
Or see this region's nucleotide sequence