Experiment: LB Anaerobic with Amoxicillin 0.00000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsA and ihfB are separated by 159 nucleotides ihfB and ycaI are separated by 207 nucleotides ycaI and msbA are separated by 36 nucleotides
b0911: rpsA - 30S ribosomal protein S1 (NCBI), at 961,218 to 962,891
rpsA
b0912: ihfB - integration host factor subunit beta (NCBI), at 963,051 to 963,335
ihfB
b0913: ycaI - orf, hypothetical protein (VIMSS), at 963,543 to 965,807
ycaI
b0914: msbA - fused lipid transporter subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 965,844 to 967,592
msbA
Position (kb) 963
964
965
966 Strain fitness (log2 ratio) -1
0
1
2 at 963.138 kb on + strand, within ihfB at 963.207 kb on + strand, within ihfB at 963.207 kb on + strand, within ihfB at 963.215 kb on - strand, within ihfB at 963.239 kb on + strand, within ihfB at 963.294 kb on - strand, within ihfB at 963.529 kb on + strand at 963.568 kb on - strand at 963.679 kb on - strand at 963.779 kb on + strand, within ycaI at 963.962 kb on - strand, within ycaI at 964.099 kb on - strand, within ycaI at 964.255 kb on + strand, within ycaI at 964.378 kb on + strand, within ycaI at 964.466 kb on - strand, within ycaI at 964.526 kb on + strand, within ycaI at 964.526 kb on + strand, within ycaI at 964.526 kb on + strand, within ycaI at 964.617 kb on + strand, within ycaI at 964.638 kb on - strand, within ycaI at 964.663 kb on - strand, within ycaI at 964.687 kb on + strand, within ycaI at 964.695 kb on - strand, within ycaI at 964.695 kb on - strand, within ycaI at 964.773 kb on + strand, within ycaI at 964.779 kb on + strand, within ycaI at 964.781 kb on - strand, within ycaI at 964.983 kb on + strand, within ycaI at 965.045 kb on + strand, within ycaI at 965.084 kb on + strand, within ycaI at 965.084 kb on + strand at 965.106 kb on + strand, within ycaI at 965.106 kb on + strand, within ycaI at 965.108 kb on + strand, within ycaI at 965.136 kb on + strand, within ycaI at 965.136 kb on + strand, within ycaI at 965.137 kb on - strand, within ycaI at 965.161 kb on - strand, within ycaI at 965.202 kb on + strand, within ycaI at 965.205 kb on - strand, within ycaI at 965.205 kb on - strand, within ycaI at 965.259 kb on - strand, within ycaI at 965.380 kb on - strand, within ycaI at 965.529 kb on + strand, within ycaI at 965.539 kb on + strand, within ycaI at 965.560 kb on + strand, within ycaI at 965.617 kb on - strand at 965.685 kb on + strand at 965.685 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.00000390625 mM remove 963,138 + ihfB b0912 0.31 +1.3 963,207 + ihfB b0912 0.55 -0.4 963,207 + ihfB b0912 0.55 -0.3 963,215 - ihfB b0912 0.58 +0.0 963,239 + ihfB b0912 0.66 -0.3 963,294 - ihfB b0912 0.85 +0.7 963,529 + -0.2 963,568 - -0.7 963,679 - +0.7 963,779 + ycaI b0913 0.10 -0.1 963,962 - ycaI b0913 0.18 +0.9 964,099 - ycaI b0913 0.25 -0.1 964,255 + ycaI b0913 0.31 +1.2 964,378 + ycaI b0913 0.37 -0.2 964,466 - ycaI b0913 0.41 -0.0 964,526 + ycaI b0913 0.43 -0.1 964,526 + ycaI b0913 0.43 -1.0 964,526 + ycaI b0913 0.43 +0.6 964,617 + ycaI b0913 0.47 +0.6 964,638 - ycaI b0913 0.48 -0.2 964,663 - ycaI b0913 0.49 +0.6 964,687 + ycaI b0913 0.51 +0.2 964,695 - ycaI b0913 0.51 +1.2 964,695 - ycaI b0913 0.51 +0.9 964,773 + ycaI b0913 0.54 -0.3 964,779 + ycaI b0913 0.55 +0.1 964,781 - ycaI b0913 0.55 +0.5 964,983 + ycaI b0913 0.64 +0.5 965,045 + ycaI b0913 0.66 +0.4 965,084 + ycaI b0913 0.68 +1.7 965,084 + +1.0 965,106 + ycaI b0913 0.69 +0.4 965,106 + ycaI b0913 0.69 +0.2 965,108 + ycaI b0913 0.69 +0.2 965,136 + ycaI b0913 0.70 +0.0 965,136 + ycaI b0913 0.70 +1.8 965,137 - ycaI b0913 0.70 -0.2 965,161 - ycaI b0913 0.71 -0.6 965,202 + ycaI b0913 0.73 +0.8 965,205 - ycaI b0913 0.73 +2.0 965,205 - ycaI b0913 0.73 +0.3 965,259 - ycaI b0913 0.76 -0.2 965,380 - ycaI b0913 0.81 +0.9 965,529 + ycaI b0913 0.88 +0.2 965,539 + ycaI b0913 0.88 -1.5 965,560 + ycaI b0913 0.89 +0.2 965,617 - -0.4 965,685 + +0.6 965,685 + +0.4
Or see this region's nucleotide sequence