Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lpxL and yceA are separated by 224 nucleotides yceA and yceI are separated by 41 nucleotides yceI and yceJ are separated by 3 nucleotides
b1054: lpxL - lipid A biosynthesis lauroyl acyltransferase (NCBI), at 1,114,885 to 1,115,805
lpxL
b1055: yceA - hypothetical protein (NCBI), at 1,116,030 to 1,117,082
yceA
b1056: yceI - hypothetical protein (NCBI), at 1,117,124 to 1,117,699
yceI
b1057: yceJ - predicted cytochrome b561 (NCBI), at 1,117,703 to 1,118,269
yceJ
Position (kb)
1116
1117
1118 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 1115.794 kb on - strand at 1115.794 kb on - strand at 1115.881 kb on + strand at 1115.889 kb on - strand at 1115.889 kb on - strand at 1115.976 kb on + strand at 1116.020 kb on + strand at 1116.021 kb on + strand at 1116.028 kb on - strand at 1116.028 kb on - strand at 1116.028 kb on - strand at 1116.036 kb on + strand at 1116.158 kb on + strand, within yceA at 1116.193 kb on - strand, within yceA at 1116.193 kb on - strand, within yceA at 1116.243 kb on + strand, within yceA at 1116.275 kb on + strand, within yceA at 1116.297 kb on - strand, within yceA at 1116.302 kb on - strand, within yceA at 1116.580 kb on - strand, within yceA at 1116.844 kb on - strand, within yceA at 1116.848 kb on + strand, within yceA at 1116.848 kb on + strand, within yceA at 1116.912 kb on - strand, within yceA at 1116.928 kb on - strand, within yceA at 1116.930 kb on + strand, within yceA at 1116.973 kb on + strand, within yceA at 1116.975 kb on + strand, within yceA at 1116.981 kb on + strand at 1116.981 kb on + strand at 1116.983 kb on - strand at 1116.989 kb on - strand at 1116.989 kb on - strand at 1117.048 kb on + strand at 1117.048 kb on + strand at 1117.054 kb on - strand at 1117.058 kb on + strand at 1117.058 kb on + strand at 1117.174 kb on + strand at 1117.199 kb on - strand, within yceI at 1117.208 kb on - strand, within yceI at 1117.240 kb on + strand, within yceI at 1117.240 kb on - strand, within yceI at 1117.247 kb on + strand, within yceI at 1117.252 kb on - strand, within yceI at 1117.252 kb on - strand, within yceI at 1117.254 kb on + strand, within yceI at 1117.254 kb on + strand, within yceI at 1117.254 kb on - strand, within yceI at 1117.254 kb on - strand, within yceI at 1117.254 kb on - strand, within yceI at 1117.262 kb on + strand, within yceI at 1117.262 kb on + strand, within yceI at 1117.306 kb on + strand, within yceI at 1117.306 kb on + strand, within yceI at 1117.307 kb on + strand, within yceI at 1117.422 kb on - strand, within yceI at 1117.422 kb on - strand, within yceI at 1117.454 kb on + strand, within yceI at 1117.458 kb on - strand, within yceI at 1117.470 kb on + strand, within yceI at 1117.478 kb on + strand, within yceI at 1117.519 kb on + strand, within yceI at 1117.570 kb on - strand, within yceI at 1117.647 kb on - strand at 1117.681 kb on - strand at 1117.681 kb on - strand at 1117.815 kb on - strand, within yceJ at 1117.815 kb on - strand, within yceJ at 1117.815 kb on - strand, within yceJ at 1117.815 kb on - strand, within yceJ at 1117.978 kb on - strand, within yceJ at 1118.027 kb on + strand, within yceJ at 1118.027 kb on + strand, within yceJ at 1118.058 kb on - strand, within yceJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 1,115,794 - -1.4 1,115,794 - +1.1 1,115,881 + -0.5 1,115,889 - +1.1 1,115,889 - +1.1 1,115,976 + -0.6 1,116,020 + -0.2 1,116,021 + +1.1 1,116,028 - +0.6 1,116,028 - +1.1 1,116,028 - -0.9 1,116,036 + +1.1 1,116,158 + yceA b1055 0.12 +7.0 1,116,193 - yceA b1055 0.15 -0.2 1,116,193 - yceA b1055 0.15 -1.2 1,116,243 + yceA b1055 0.20 +0.5 1,116,275 + yceA b1055 0.23 +1.4 1,116,297 - yceA b1055 0.25 +0.1 1,116,302 - yceA b1055 0.26 -1.2 1,116,580 - yceA b1055 0.52 +0.8 1,116,844 - yceA b1055 0.77 -0.1 1,116,848 + yceA b1055 0.78 -0.6 1,116,848 + yceA b1055 0.78 -0.2 1,116,912 - yceA b1055 0.84 +0.1 1,116,928 - yceA b1055 0.85 -0.3 1,116,930 + yceA b1055 0.85 +0.9 1,116,973 + yceA b1055 0.90 -0.6 1,116,975 + yceA b1055 0.90 +0.1 1,116,981 + +0.8 1,116,981 + +0.7 1,116,983 - +2.7 1,116,989 - -0.4 1,116,989 - +1.1 1,117,048 + -0.1 1,117,048 + -0.9 1,117,054 - +1.1 1,117,058 + +0.4 1,117,058 + +0.4 1,117,174 + -0.4 1,117,199 - yceI b1056 0.13 +0.1 1,117,208 - yceI b1056 0.15 -0.7 1,117,240 + yceI b1056 0.20 -0.5 1,117,240 - yceI b1056 0.20 +0.7 1,117,247 + yceI b1056 0.21 +0.1 1,117,252 - yceI b1056 0.22 -0.7 1,117,252 - yceI b1056 0.22 -0.3 1,117,254 + yceI b1056 0.23 +1.6 1,117,254 + yceI b1056 0.23 +1.6 1,117,254 - yceI b1056 0.23 +1.2 1,117,254 - yceI b1056 0.23 +1.6 1,117,254 - yceI b1056 0.23 -2.0 1,117,262 + yceI b1056 0.24 +0.2 1,117,262 + yceI b1056 0.24 -0.2 1,117,306 + yceI b1056 0.32 -0.7 1,117,306 + yceI b1056 0.32 -1.2 1,117,307 + yceI b1056 0.32 -0.1 1,117,422 - yceI b1056 0.52 -1.2 1,117,422 - yceI b1056 0.52 +0.4 1,117,454 + yceI b1056 0.57 -0.4 1,117,458 - yceI b1056 0.58 +0.1 1,117,470 + yceI b1056 0.60 -0.9 1,117,478 + yceI b1056 0.61 +1.7 1,117,519 + yceI b1056 0.69 +0.1 1,117,570 - yceI b1056 0.77 -0.3 1,117,647 - +0.1 1,117,681 - +0.7 1,117,681 - -1.2 1,117,815 - yceJ b1057 0.20 -0.2 1,117,815 - yceJ b1057 0.20 +0.6 1,117,815 - yceJ b1057 0.20 -2.3 1,117,815 - yceJ b1057 0.20 -1.4 1,117,978 - yceJ b1057 0.49 +0.1 1,118,027 + yceJ b1057 0.57 +1.1 1,118,027 + yceJ b1057 0.57 +0.1 1,118,058 - yceJ b1057 0.63 -1.6
Or see this region's nucleotide sequence