Strain Fitness in Escherichia coli BW25113 around b2558

Experiment: LB Anaerobic with Ticarcillin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpurL and mltF are separated by 257 nucleotidesmltF and tadA overlap by 4 nucleotidestadA and yfhB are separated by 57 nucleotides b2557: purL - phosphoribosylformylglycinamidine synthase (NCBI), at 2,689,678 to 2,693,565 purL b2558: mltF - predicted periplasmic binding protein/transglycosylase (RefSeq), at 2,693,823 to 2,695,379 mltF b2559: tadA - tRNA-specific adenosine deaminase (RefSeq), at 2,695,376 to 2,695,879 tadA b2560: yfhB - orf, hypothetical protein (VIMSS), at 2,695,937 to 2,696,572 yfhB Position (kb) 2693 2694 2695 2696Strain fitness (log2 ratio) -1 0 1 2at 2692.940 kb on + strand, within purLat 2692.973 kb on - strand, within purLat 2693.040 kb on + strand, within purLat 2693.055 kb on + strand, within purLat 2693.288 kb on - strandat 2693.315 kb on + strandat 2693.386 kb on + strandat 2693.577 kb on + strandat 2693.888 kb on - strandat 2693.888 kb on - strandat 2693.916 kb on + strandat 2693.916 kb on + strandat 2694.119 kb on + strand, within mltFat 2694.152 kb on + strand, within mltFat 2694.152 kb on + strand, within mltFat 2694.152 kb on + strand, within mltFat 2694.158 kb on - strand, within mltFat 2694.158 kb on - strand, within mltFat 2694.194 kb on + strand, within mltFat 2694.194 kb on + strand, within mltFat 2694.212 kb on - strand, within mltFat 2694.212 kb on - strand, within mltFat 2694.236 kb on + strand, within mltFat 2694.252 kb on - strand, within mltFat 2694.283 kb on - strand, within mltFat 2694.290 kb on + strand, within mltFat 2694.310 kb on - strand, within mltFat 2694.319 kb on - strand, within mltFat 2694.433 kb on - strand, within mltFat 2694.438 kb on + strand, within mltFat 2694.438 kb on + strand, within mltFat 2694.592 kb on + strand, within mltFat 2694.592 kb on + strand, within mltFat 2694.611 kb on + strand, within mltFat 2694.684 kb on - strand, within mltFat 2694.692 kb on + strand, within mltFat 2694.716 kb on + strand, within mltFat 2694.791 kb on + strand, within mltFat 2694.792 kb on - strand, within mltFat 2694.824 kb on + strand, within mltFat 2694.847 kb on + strand, within mltFat 2694.891 kb on - strand, within mltFat 2694.891 kb on - strand, within mltFat 2694.931 kb on - strand, within mltFat 2694.931 kb on - strand, within mltFat 2694.969 kb on + strand, within mltFat 2694.971 kb on + strand, within mltFat 2694.976 kb on + strand, within mltFat 2695.086 kb on + strand, within mltFat 2695.109 kb on + strand, within mltFat 2695.125 kb on + strand, within mltFat 2695.138 kb on - strand, within mltFat 2695.203 kb on - strand, within mltFat 2695.203 kb on - strand, within mltFat 2695.252 kb on + strandat 2695.252 kb on + strandat 2695.256 kb on - strandat 2695.260 kb on - strandat 2695.328 kb on + strandat 2695.365 kb on - strandat 2695.945 kb on - strandat 2695.945 kb on - strandat 2695.966 kb on + strandat 2695.966 kb on + strandat 2695.968 kb on + strandat 2695.968 kb on + strandat 2696.004 kb on + strand, within yfhBat 2696.004 kb on + strand, within yfhBat 2696.014 kb on + strand, within yfhBat 2696.014 kb on + strand, within yfhBat 2696.024 kb on + strand, within yfhBat 2696.044 kb on + strand, within yfhBat 2696.044 kb on - strand, within yfhBat 2696.050 kb on - strand, within yfhBat 2696.077 kb on - strand, within yfhBat 2696.077 kb on - strand, within yfhBat 2696.089 kb on + strand, within yfhBat 2696.089 kb on + strand, within yfhBat 2696.164 kb on - strand, within yfhB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ticarcillin 0.000015625 mM
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2,692,940 + purL b2557 0.84 +0.9
2,692,973 - purL b2557 0.85 +0.6
2,693,040 + purL b2557 0.86 +0.3
2,693,055 + purL b2557 0.87 -1.0
2,693,288 - +0.3
2,693,315 + -0.0
2,693,386 + +0.2
2,693,577 + +0.3
2,693,888 - +0.5
2,693,888 - -0.1
2,693,916 + -0.1
2,693,916 + -0.4
2,694,119 + mltF b2558 0.19 +1.0
2,694,152 + mltF b2558 0.21 -0.5
2,694,152 + mltF b2558 0.21 -0.3
2,694,152 + mltF b2558 0.21 +0.4
2,694,158 - mltF b2558 0.22 +0.4
2,694,158 - mltF b2558 0.22 +0.2
2,694,194 + mltF b2558 0.24 +0.9
2,694,194 + mltF b2558 0.24 -0.7
2,694,212 - mltF b2558 0.25 +0.5
2,694,212 - mltF b2558 0.25 +0.0
2,694,236 + mltF b2558 0.27 +0.4
2,694,252 - mltF b2558 0.28 +0.2
2,694,283 - mltF b2558 0.30 -1.5
2,694,290 + mltF b2558 0.30 -0.2
2,694,310 - mltF b2558 0.31 -0.1
2,694,319 - mltF b2558 0.32 -0.2
2,694,433 - mltF b2558 0.39 -1.0
2,694,438 + mltF b2558 0.39 +0.2
2,694,438 + mltF b2558 0.39 +0.1
2,694,592 + mltF b2558 0.49 -1.4
2,694,592 + mltF b2558 0.49 +1.1
2,694,611 + mltF b2558 0.51 -0.3
2,694,684 - mltF b2558 0.55 -0.9
2,694,692 + mltF b2558 0.56 +0.6
2,694,716 + mltF b2558 0.57 +0.6
2,694,791 + mltF b2558 0.62 -0.8
2,694,792 - mltF b2558 0.62 -0.9
2,694,824 + mltF b2558 0.64 -0.6
2,694,847 + mltF b2558 0.66 +1.2
2,694,891 - mltF b2558 0.69 +2.2
2,694,891 - mltF b2558 0.69 -1.7
2,694,931 - mltF b2558 0.71 +0.1
2,694,931 - mltF b2558 0.71 +0.1
2,694,969 + mltF b2558 0.74 +0.4
2,694,971 + mltF b2558 0.74 -0.7
2,694,976 + mltF b2558 0.74 -0.2
2,695,086 + mltF b2558 0.81 +0.5
2,695,109 + mltF b2558 0.83 +1.3
2,695,125 + mltF b2558 0.84 -0.5
2,695,138 - mltF b2558 0.84 -0.2
2,695,203 - mltF b2558 0.89 -0.5
2,695,203 - mltF b2558 0.89 +0.6
2,695,252 + -0.3
2,695,252 + -0.7
2,695,256 - +0.3
2,695,260 - +0.1
2,695,328 + -0.1
2,695,365 - +0.8
2,695,945 - -0.7
2,695,945 - -1.4
2,695,966 + +0.6
2,695,966 + -1.0
2,695,968 + -0.1
2,695,968 + +0.9
2,696,004 + yfhB b2560 0.11 +1.1
2,696,004 + yfhB b2560 0.11 +0.2
2,696,014 + yfhB b2560 0.12 -0.3
2,696,014 + yfhB b2560 0.12 +0.1
2,696,024 + yfhB b2560 0.14 -0.2
2,696,044 + yfhB b2560 0.17 -0.3
2,696,044 - yfhB b2560 0.17 -0.2
2,696,050 - yfhB b2560 0.18 -0.5
2,696,077 - yfhB b2560 0.22 -0.7
2,696,077 - yfhB b2560 0.22 -0.4
2,696,089 + yfhB b2560 0.24 -0.1
2,696,089 + yfhB b2560 0.24 -0.3
2,696,164 - yfhB b2560 0.36 +0.4

Or see this region's nucleotide sequence