Strain Fitness in Escherichia coli BW25113 around b2435

Experiment: LB Anaerobic with Ticarcillin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntb2433 and b2434 overlap by 14 nucleotidesb2434 and amiA are separated by 213 nucleotidesamiA and hemF are separated by 3 nucleotideshemF and yfeG are separated by 5 nucleotides b2433: b2433 - orf, hypothetical protein (VIMSS), at 2,549,299 to 2,549,748 b2433 b2434: b2434 - orf, hypothetical protein (VIMSS), at 2,549,735 to 2,550,160 b2434 b2435: amiA - N-acetylmuramoyl-l-alanine amidase I (NCBI), at 2,550,374 to 2,551,243 amiA b2436: hemF - coproporphyrinogen III oxidase (NCBI), at 2,551,247 to 2,552,146 hemF b2437: yfeG - predicted DNA-binding transcriptional regulator (NCBI), at 2,552,152 to 2,553,204 yfeG Position (kb) 2550 2551 2552Strain fitness (log2 ratio) -1 0 1 2at 2549.388 kb on + strand, within b2433at 2549.645 kb on + strand, within b2433at 2549.680 kb on + strand, within b2433at 2549.680 kb on + strand, within b2433at 2549.746 kb on - strandat 2549.795 kb on + strand, within b2434at 2549.833 kb on - strand, within b2434at 2549.864 kb on + strand, within b2434at 2550.001 kb on + strand, within b2434at 2550.037 kb on + strand, within b2434at 2550.357 kb on + strandat 2550.357 kb on + strandat 2550.381 kb on + strandat 2550.597 kb on - strand, within amiAat 2550.631 kb on - strand, within amiAat 2550.747 kb on + strand, within amiAat 2550.779 kb on + strand, within amiAat 2550.779 kb on + strand, within amiAat 2551.032 kb on + strand, within amiAat 2551.066 kb on - strand, within amiAat 2551.197 kb on + strandat 2551.266 kb on + strandat 2551.295 kb on - strandat 2551.295 kb on - strandat 2551.355 kb on - strand, within hemFat 2551.444 kb on + strand, within hemFat 2551.444 kb on + strand, within hemFat 2551.531 kb on - strand, within hemFat 2551.643 kb on - strand, within hemFat 2551.645 kb on + strand, within hemFat 2551.678 kb on + strand, within hemFat 2551.678 kb on + strand, within hemFat 2551.837 kb on + strand, within hemFat 2551.837 kb on + strand, within hemFat 2552.065 kb on - strandat 2552.065 kb on - strandat 2552.087 kb on + strandat 2552.087 kb on + strandat 2552.141 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ticarcillin 0.000015625 mM
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2,549,388 + b2433 b2433 0.20 +0.8
2,549,645 + b2433 b2433 0.77 +0.8
2,549,680 + b2433 b2433 0.85 -1.0
2,549,680 + b2433 b2433 0.85 -0.2
2,549,746 - -0.4
2,549,795 + b2434 b2434 0.14 -1.2
2,549,833 - b2434 b2434 0.23 -0.1
2,549,864 + b2434 b2434 0.30 -1.0
2,550,001 + b2434 b2434 0.62 -0.2
2,550,037 + b2434 b2434 0.71 -0.3
2,550,357 + +0.0
2,550,357 + +0.8
2,550,381 + +2.4
2,550,597 - amiA b2435 0.26 +0.1
2,550,631 - amiA b2435 0.30 +0.4
2,550,747 + amiA b2435 0.43 -0.0
2,550,779 + amiA b2435 0.47 +0.7
2,550,779 + amiA b2435 0.47 -0.7
2,551,032 + amiA b2435 0.76 +0.4
2,551,066 - amiA b2435 0.80 +1.2
2,551,197 + -0.7
2,551,266 + +0.4
2,551,295 - -0.2
2,551,295 - +0.8
2,551,355 - hemF b2436 0.12 +0.0
2,551,444 + hemF b2436 0.22 +1.2
2,551,444 + hemF b2436 0.22 +1.3
2,551,531 - hemF b2436 0.32 +0.5
2,551,643 - hemF b2436 0.44 +0.5
2,551,645 + hemF b2436 0.44 -0.7
2,551,678 + hemF b2436 0.48 +0.0
2,551,678 + hemF b2436 0.48 +0.0
2,551,837 + hemF b2436 0.66 +0.3
2,551,837 + hemF b2436 0.66 -0.2
2,552,065 - +0.4
2,552,065 - +0.6
2,552,087 + +0.0
2,552,087 + -0.0
2,552,141 + -0.0

Or see this region's nucleotide sequence