Strain Fitness in Escherichia coli BW25113 around b0085

Experiment: LB Anaerobic with Ticarcillin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntftsI and murE overlap by 14 nucleotidesmurE and murF overlap by 4 nucleotides b0084: ftsI - transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3) (NCBI), at 91,413 to 93,179 ftsI b0085: murE - UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase (NCBI), at 93,166 to 94,653 murE b0086: murF - UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D- alanine ligase (NCBI), at 94,650 to 96,008 murF Position (kb) 93 94 95Strain fitness (log2 ratio) -1 0 1at 93.112 kb on + strandat 93.112 kb on + strandat 93.112 kb on + strandat 93.113 kb on + strandat 93.113 kb on + strandat 93.113 kb on + strandat 93.153 kb on + strandat 93.219 kb on + strandat 94.617 kb on + strandat 94.634 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ticarcillin 0.000015625 mM
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93,112 + +0.4
93,112 + +0.3
93,112 + -0.0
93,113 + +0.9
93,113 + +0.7
93,113 + -0.6
93,153 + -0.3
93,219 + +1.0
94,617 + +0.3
94,634 + -0.3

Or see this region's nucleotide sequence