Experiment: Growth on radish seeds for 72 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_11855 and PS417_11860 are separated by 3 nucleotides PS417_11860 and PS417_11865 are separated by 28 nucleotides PS417_11865 and PS417_11870 are separated by 149 nucleotides
PS417_11855: PS417_11855 - Myo-inosose-2 dehydratase (EC 4.2.1.44) (from data) , at 2,581,055 to 2,581,942
_11855
PS417_11860: PS417_11860 - 5-deoxy-D-glucuronate isomerase (EC 5.3.1.30) (from data) , at 2,581,946 to 2,582,734
_11860
PS417_11865: PS417_11865 - xylose isomerase, at 2,582,763 to 2,583,584
_11865
PS417_11870: PS417_11870 - 3D-(3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase IolD (EC 3.7.1.22) (from data) , at 2,583,734 to 2,585,665
_11870
Position (kb)
2581
2582
2583 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2580.954 kb on + strand at 2581.005 kb on + strand at 2581.005 kb on + strand at 2581.523 kb on - strand, within PS417_11855 at 2581.532 kb on + strand, within PS417_11855 at 2581.532 kb on + strand, within PS417_11855 at 2581.616 kb on + strand, within PS417_11855 at 2581.617 kb on - strand, within PS417_11855 at 2581.617 kb on - strand, within PS417_11855 at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.883 kb on + strand at 2581.884 kb on - strand at 2581.884 kb on - strand at 2581.884 kb on - strand at 2581.884 kb on - strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.885 kb on + strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.886 kb on - strand at 2581.889 kb on + strand at 2581.890 kb on - strand at 2581.890 kb on - strand at 2582.015 kb on + strand at 2582.016 kb on - strand at 2582.242 kb on - strand, within PS417_11860 at 2582.406 kb on + strand, within PS417_11860 at 2582.407 kb on - strand, within PS417_11860 at 2582.411 kb on - strand, within PS417_11860 at 2582.496 kb on - strand, within PS417_11860 at 2582.651 kb on + strand, within PS417_11860 at 2582.652 kb on - strand, within PS417_11860 at 2582.652 kb on - strand at 2582.652 kb on - strand, within PS417_11860 at 2582.776 kb on + strand at 2583.034 kb on - strand, within PS417_11865 at 2583.350 kb on + strand, within PS417_11865 at 2583.351 kb on - strand, within PS417_11865 at 2583.351 kb on - strand, within PS417_11865 at 2583.648 kb on + strand at 2583.649 kb on - strand at 2583.649 kb on - strand at 2583.720 kb on + strand at 2583.721 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Growth on radish seeds for 72 hours remove 2,580,954 + +1.6 2,581,005 + -0.4 2,581,005 + -1.4 2,581,523 - PS417_11855 0.53 -0.2 2,581,532 + PS417_11855 0.54 +2.1 2,581,532 + PS417_11855 0.54 +0.2 2,581,616 + PS417_11855 0.63 +0.1 2,581,617 - PS417_11855 0.63 -0.5 2,581,617 - PS417_11855 0.63 -1.0 2,581,883 + -1.0 2,581,883 + -0.8 2,581,883 + -0.6 2,581,883 + +1.9 2,581,883 + -1.0 2,581,884 - +0.6 2,581,884 - -0.7 2,581,884 - -0.4 2,581,884 - +0.6 2,581,885 + -1.0 2,581,885 + +0.3 2,581,885 + -0.5 2,581,885 + +1.1 2,581,885 + -1.0 2,581,885 + -0.7 2,581,885 + +3.3 2,581,886 - +0.6 2,581,886 - +0.0 2,581,886 - -0.0 2,581,886 - +0.4 2,581,886 - +0.8 2,581,886 - -1.0 2,581,886 - -1.8 2,581,889 + -0.6 2,581,890 - -0.4 2,581,890 - -1.6 2,582,015 + -0.8 2,582,016 - -1.0 2,582,242 - PS417_11860 0.38 -0.8 2,582,406 + PS417_11860 0.58 +0.0 2,582,407 - PS417_11860 0.58 -0.9 2,582,411 - PS417_11860 0.59 +0.6 2,582,496 - PS417_11860 0.70 -1.8 2,582,651 + PS417_11860 0.89 -0.3 2,582,652 - PS417_11860 0.89 +2.6 2,582,652 - +0.6 2,582,652 - PS417_11860 0.89 +0.3 2,582,776 + -0.7 2,583,034 - PS417_11865 0.33 -0.0 2,583,350 + PS417_11865 0.71 +0.6 2,583,351 - PS417_11865 0.72 +1.6 2,583,351 - PS417_11865 0.72 -0.0 2,583,648 + +1.6 2,583,649 - +1.9 2,583,649 - +0.1 2,583,720 + +0.6 2,583,721 - -0.4
Or see this region's nucleotide sequence