Strain Fitness in Pseudomonas simiae WCS417 around PS417_11350

Experiment: Growth on radish seeds for 72 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPS417_11345 and PS417_11350 are separated by 24 nucleotidesPS417_11350 and PS417_11355 are separated by 11 nucleotidesPS417_11355 and PS417_11360 are separated by 3 nucleotides PS417_11345: PS417_11345 - membrane protein, at 2,443,306 to 2,444,889 _11345 PS417_11350: PS417_11350 - biopolymer transporter ExbB, at 2,444,914 to 2,446,713 _11350 PS417_11355: PS417_11355 - biopolymer transporter ExbD, at 2,446,725 to 2,447,150 _11355 PS417_11360: PS417_11360 - energy transducer TonB, at 2,447,154 to 2,447,858 _11360 Position (kb) 2444 2445 2446 2447Strain fitness (log2 ratio) -1 0 1 2at 2443.934 kb on - strand, within PS417_11345at 2444.450 kb on - strandat 2444.656 kb on + strand, within PS417_11345at 2444.656 kb on + strand, within PS417_11345at 2444.657 kb on - strand, within PS417_11345at 2444.657 kb on - strand, within PS417_11345at 2445.271 kb on + strand, within PS417_11350at 2445.272 kb on - strand, within PS417_11350at 2445.272 kb on - strand, within PS417_11350at 2445.272 kb on - strand, within PS417_11350at 2445.331 kb on + strand, within PS417_11350at 2446.169 kb on - strand, within PS417_11350at 2447.038 kb on + strandat 2447.039 kb on - strand, within PS417_11355at 2447.596 kb on - strand, within PS417_11360at 2447.598 kb on - strand, within PS417_11360at 2447.619 kb on + strand, within PS417_11360

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Per-strain Table

Position Strand Gene LocusTag Fraction Growth on radish seeds for 72 hours
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2,443,934 - PS417_11345 0.40 +0.2
2,444,450 - +1.6
2,444,656 + PS417_11345 0.85 +2.0
2,444,656 + PS417_11345 0.85 -1.4
2,444,657 - PS417_11345 0.85 -0.6
2,444,657 - PS417_11345 0.85 +0.0
2,445,271 + PS417_11350 0.20 -0.1
2,445,272 - PS417_11350 0.20 -1.0
2,445,272 - PS417_11350 0.20 +0.6
2,445,272 - PS417_11350 0.20 -0.6
2,445,331 + PS417_11350 0.23 -0.5
2,446,169 - PS417_11350 0.70 +0.3
2,447,038 + +0.0
2,447,039 - PS417_11355 0.74 -0.6
2,447,596 - PS417_11360 0.63 -0.4
2,447,598 - PS417_11360 0.63 -0.6
2,447,619 + PS417_11360 0.66 +0.5

Or see this region's nucleotide sequence