Strain Fitness in Escherichia coli BW25113 around b0436

Experiment: LB Anaerobic with Ticarcillin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyajG and bolA are separated by 304 nucleotidesbolA and tig are separated by 343 nucleotidestig and clpP are separated by 245 nucleotidesclpP and clpX are separated by 125 nucleotides b0434: yajG - putative polymerase/proteinase (VIMSS), at 452,813 to 453,391 yajG b0435: bolA - possible regulator of murein genes (VIMSS), at 453,696 to 454,013 bolA b0436: tig - trigger factor (NCBI), at 454,357 to 455,655 tig b0437: clpP - ATP-dependent Clp protease proteolytic subunit (NCBI), at 455,901 to 456,524 clpP b0438: clpX - ATP-dependent protease ATP-binding subunit (NCBI), at 456,650 to 457,924 clpX Position (kb) 454 455 456Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 453.478 kb on - strandat 453.486 kb on - strandat 453.486 kb on - strandat 453.538 kb on + strandat 453.647 kb on + strandat 453.668 kb on + strandat 453.668 kb on + strandat 453.703 kb on + strandat 453.703 kb on + strandat 453.705 kb on - strandat 453.705 kb on - strandat 453.708 kb on - strandat 453.765 kb on + strand, within bolAat 453.794 kb on - strand, within bolAat 453.794 kb on - strand, within bolAat 453.794 kb on - strand, within bolAat 453.832 kb on - strand, within bolAat 453.835 kb on + strand, within bolAat 453.835 kb on + strand, within bolAat 453.929 kb on + strand, within bolAat 453.937 kb on - strand, within bolAat 453.937 kb on - strand, within bolAat 453.939 kb on + strand, within bolAat 453.939 kb on + strand, within bolAat 453.957 kb on + strand, within bolAat 453.996 kb on + strandat 454.039 kb on + strandat 454.128 kb on - strandat 454.177 kb on - strandat 454.177 kb on - strandat 454.207 kb on + strandat 454.264 kb on - strandat 454.299 kb on + strandat 454.299 kb on + strandat 454.302 kb on - strandat 454.334 kb on + strandat 454.334 kb on - strandat 454.334 kb on - strandat 454.386 kb on - strandat 454.387 kb on - strandat 454.452 kb on + strandat 454.452 kb on + strandat 454.452 kb on + strandat 454.460 kb on - strandat 454.460 kb on - strandat 454.543 kb on + strand, within tigat 454.543 kb on + strand, within tigat 454.561 kb on + strand, within tigat 454.578 kb on - strand, within tigat 454.747 kb on + strand, within tigat 454.752 kb on + strand, within tigat 454.776 kb on + strand, within tigat 454.776 kb on + strand, within tigat 454.776 kb on + strand, within tigat 454.924 kb on + strand, within tigat 454.924 kb on + strand, within tigat 454.932 kb on - strand, within tigat 455.024 kb on + strand, within tigat 455.024 kb on + strand, within tigat 455.089 kb on - strand, within tigat 455.089 kb on - strand, within tigat 455.128 kb on + strand, within tigat 455.128 kb on + strand, within tigat 455.128 kb on + strand, within tigat 455.217 kb on + strand, within tigat 455.314 kb on - strand, within tigat 455.314 kb on - strand, within tigat 455.439 kb on + strand, within tigat 455.467 kb on + strand, within tigat 455.563 kb on - strandat 455.563 kb on - strandat 455.812 kb on - strandat 455.824 kb on - strandat 455.827 kb on - strandat 455.852 kb on + strandat 455.852 kb on + strandat 455.921 kb on + strandat 455.958 kb on - strandat 456.018 kb on - strand, within clpPat 456.053 kb on - strand, within clpPat 456.141 kb on + strand, within clpPat 456.142 kb on - strand, within clpPat 456.162 kb on + strand, within clpPat 456.162 kb on - strand, within clpPat 456.162 kb on - strand, within clpPat 456.168 kb on + strand, within clpPat 456.170 kb on + strand, within clpPat 456.217 kb on - strand, within clpPat 456.220 kb on - strand, within clpPat 456.222 kb on - strand, within clpPat 456.222 kb on - strand, within clpPat 456.320 kb on - strand, within clpPat 456.327 kb on - strand, within clpPat 456.345 kb on + strand, within clpPat 456.383 kb on - strand, within clpPat 456.396 kb on + strand, within clpPat 456.422 kb on - strand, within clpPat 456.429 kb on - strand, within clpPat 456.429 kb on - strand, within clpPat 456.429 kb on - strand, within clpPat 456.429 kb on - strand, within clpPat 456.439 kb on - strand, within clpPat 456.480 kb on + strandat 456.504 kb on + strandat 456.541 kb on + strandat 456.610 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ticarcillin 0.00003125 mM
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453,478 - -0.9
453,486 - -0.3
453,486 - -0.1
453,538 + +0.5
453,647 + -0.6
453,668 + +0.3
453,668 + +1.0
453,703 + +1.0
453,703 + -1.2
453,705 - -0.2
453,705 - -0.2
453,708 - -0.2
453,765 + bolA b0435 0.22 +0.9
453,794 - bolA b0435 0.31 -0.3
453,794 - bolA b0435 0.31 +0.2
453,794 - bolA b0435 0.31 +1.2
453,832 - bolA b0435 0.43 -0.4
453,835 + bolA b0435 0.44 +1.3
453,835 + bolA b0435 0.44 +0.2
453,929 + bolA b0435 0.73 +0.1
453,937 - bolA b0435 0.76 +2.0
453,937 - bolA b0435 0.76 +1.3
453,939 + bolA b0435 0.76 -0.1
453,939 + bolA b0435 0.76 +0.4
453,957 + bolA b0435 0.82 +0.3
453,996 + -0.1
454,039 + +0.2
454,128 - -0.3
454,177 - -0.3
454,177 - +0.5
454,207 + -0.7
454,264 - -0.9
454,299 + +0.0
454,299 + -1.2
454,302 - -2.5
454,334 + -2.1
454,334 - -1.0
454,334 - -1.8
454,386 - -1.2
454,387 - +1.0
454,452 + -1.6
454,452 + -0.0
454,452 + -1.6
454,460 - -2.8
454,460 - -1.2
454,543 + tig b0436 0.14 -1.8
454,543 + tig b0436 0.14 -2.1
454,561 + tig b0436 0.16 -0.6
454,578 - tig b0436 0.17 -1.1
454,747 + tig b0436 0.30 -1.0
454,752 + tig b0436 0.30 -3.0
454,776 + tig b0436 0.32 -3.0
454,776 + tig b0436 0.32 -1.4
454,776 + tig b0436 0.32 -1.2
454,924 + tig b0436 0.44 -0.6
454,924 + tig b0436 0.44 -0.2
454,932 - tig b0436 0.44 -0.3
455,024 + tig b0436 0.51 -1.2
455,024 + tig b0436 0.51 -1.1
455,089 - tig b0436 0.56 -0.2
455,089 - tig b0436 0.56 -1.3
455,128 + tig b0436 0.59 +0.3
455,128 + tig b0436 0.59 -1.3
455,128 + tig b0436 0.59 -2.3
455,217 + tig b0436 0.66 -1.8
455,314 - tig b0436 0.74 +1.4
455,314 - tig b0436 0.74 -3.0
455,439 + tig b0436 0.83 -0.6
455,467 + tig b0436 0.85 -2.2
455,563 - -1.3
455,563 - -2.2
455,812 - +1.0
455,824 - -0.8
455,827 - -2.0
455,852 + +0.3
455,852 + +0.6
455,921 + +0.1
455,958 - +2.0
456,018 - clpP b0437 0.19 -0.6
456,053 - clpP b0437 0.24 -0.0
456,141 + clpP b0437 0.38 -2.3
456,142 - clpP b0437 0.39 -1.3
456,162 + clpP b0437 0.42 -0.2
456,162 - clpP b0437 0.42 -1.3
456,162 - clpP b0437 0.42 -1.0
456,168 + clpP b0437 0.43 -0.9
456,170 + clpP b0437 0.43 +0.5
456,217 - clpP b0437 0.51 +1.0
456,220 - clpP b0437 0.51 +0.7
456,222 - clpP b0437 0.51 -0.0
456,222 - clpP b0437 0.51 +0.3
456,320 - clpP b0437 0.67 -0.6
456,327 - clpP b0437 0.68 -1.6
456,345 + clpP b0437 0.71 -0.8
456,383 - clpP b0437 0.77 -1.0
456,396 + clpP b0437 0.79 -1.0
456,422 - clpP b0437 0.83 +0.3
456,429 - clpP b0437 0.85 -0.8
456,429 - clpP b0437 0.85 +1.5
456,429 - clpP b0437 0.85 -1.6
456,429 - clpP b0437 0.85 +1.6
456,439 - clpP b0437 0.86 -0.0
456,480 + -0.2
456,504 + +0.2
456,541 + -0.3
456,610 + +1.6

Or see this region's nucleotide sequence