Strain Fitness in Escherichia coli BW25113 around b4646

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhdH and yrdF are separated by 36 nucleotidesyrdF and yrdE are separated by 0 nucleotidesyrdE and accB are separated by 404 nucleotidesaccB and accC are separated by 10 nucleotides b3253: yhdH - predicted oxidoreductase, Zn-dependent and NAD(P)-binding (NCBI), at 3,401,506 to 3,402,480 yhdH b4697: yrdF - no description, at 3,402,517 to 3,402,659 yrdF b4646: yrdE - no description, at 3,402,660 to 3,403,053 yrdE b3255: accB - acetyl-CoA carboxylase (NCBI), at 3,403,458 to 3,403,928 accB b3256: accC - acetyl-CoA carboxylase (NCBI), at 3,403,939 to 3,405,288 accC Position (kb) 3402 3403 3404Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 3401.713 kb on - strand, within yhdHat 3401.723 kb on - strand, within yhdHat 3401.723 kb on - strand, within yhdHat 3401.792 kb on - strand, within yhdHat 3401.792 kb on - strand, within yhdHat 3401.792 kb on - strand, within yhdHat 3401.851 kb on - strand, within yhdHat 3401.851 kb on - strand, within yhdHat 3401.965 kb on + strand, within yhdHat 3402.006 kb on - strand, within yhdHat 3402.019 kb on - strand, within yhdHat 3402.019 kb on - strand, within yhdHat 3402.034 kb on + strand, within yhdHat 3402.071 kb on + strand, within yhdHat 3402.076 kb on + strand, within yhdHat 3402.076 kb on + strand, within yhdHat 3402.160 kb on + strand, within yhdHat 3402.180 kb on - strand, within yhdHat 3402.220 kb on - strand, within yhdHat 3402.243 kb on - strand, within yhdHat 3402.296 kb on + strand, within yhdHat 3402.296 kb on + strand, within yhdHat 3402.296 kb on + strand, within yhdHat 3402.300 kb on + strand, within yhdHat 3402.300 kb on + strand, within yhdHat 3402.305 kb on + strand, within yhdHat 3402.305 kb on + strand, within yhdHat 3402.319 kb on + strand, within yhdHat 3402.327 kb on - strand, within yhdHat 3402.327 kb on - strand, within yhdHat 3402.381 kb on - strand, within yhdHat 3402.381 kb on - strand, within yhdHat 3402.448 kb on + strandat 3402.450 kb on - strandat 3402.473 kb on - strandat 3402.473 kb on - strandat 3402.496 kb on + strandat 3402.496 kb on + strandat 3402.588 kb on + strand, within yrdFat 3402.595 kb on + strand, within yrdFat 3402.677 kb on + strandat 3402.715 kb on - strand, within yrdEat 3402.715 kb on - strand, within yrdEat 3402.726 kb on + strand, within yrdEat 3402.726 kb on + strand, within yrdEat 3402.741 kb on - strand, within yrdEat 3402.741 kb on - strand, within yrdEat 3402.812 kb on + strand, within yrdEat 3402.836 kb on + strand, within yrdEat 3403.197 kb on - strandat 3403.370 kb on + strandat 3403.372 kb on + strandat 3403.372 kb on + strandat 3403.374 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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3,401,713 - yhdH b3253 0.21 -1.0
3,401,723 - yhdH b3253 0.22 +0.3
3,401,723 - yhdH b3253 0.22 +1.1
3,401,792 - yhdH b3253 0.29 -1.4
3,401,792 - yhdH b3253 0.29 -1.9
3,401,792 - yhdH b3253 0.29 -2.0
3,401,851 - yhdH b3253 0.35 -2.8
3,401,851 - yhdH b3253 0.35 +0.8
3,401,965 + yhdH b3253 0.47 -1.4
3,402,006 - yhdH b3253 0.51 -1.1
3,402,019 - yhdH b3253 0.53 +0.1
3,402,019 - yhdH b3253 0.53 -1.5
3,402,034 + yhdH b3253 0.54 -1.6
3,402,071 + yhdH b3253 0.58 -1.3
3,402,076 + yhdH b3253 0.58 -2.2
3,402,076 + yhdH b3253 0.58 -1.0
3,402,160 + yhdH b3253 0.67 -1.5
3,402,180 - yhdH b3253 0.69 -0.3
3,402,220 - yhdH b3253 0.73 -3.7
3,402,243 - yhdH b3253 0.76 -0.4
3,402,296 + yhdH b3253 0.81 -1.0
3,402,296 + yhdH b3253 0.81 -0.5
3,402,296 + yhdH b3253 0.81 -1.9
3,402,300 + yhdH b3253 0.81 -1.0
3,402,300 + yhdH b3253 0.81 -1.5
3,402,305 + yhdH b3253 0.82 -1.5
3,402,305 + yhdH b3253 0.82 -0.7
3,402,319 + yhdH b3253 0.83 -1.4
3,402,327 - yhdH b3253 0.84 -1.3
3,402,327 - yhdH b3253 0.84 -0.3
3,402,381 - yhdH b3253 0.90 -0.9
3,402,381 - yhdH b3253 0.90 -3.1
3,402,448 + -1.4
3,402,450 - -0.6
3,402,473 - -0.4
3,402,473 - +0.1
3,402,496 + -0.9
3,402,496 + -1.0
3,402,588 + yrdF b4697 0.50 -0.3
3,402,595 + yrdF b4697 0.55 +0.2
3,402,677 + +0.7
3,402,715 - yrdE b4646 0.14 -1.9
3,402,715 - yrdE b4646 0.14 -0.5
3,402,726 + yrdE b4646 0.17 -0.1
3,402,726 + yrdE b4646 0.17 +0.6
3,402,741 - yrdE b4646 0.21 -0.3
3,402,741 - yrdE b4646 0.21 -0.3
3,402,812 + yrdE b4646 0.39 +4.9
3,402,836 + yrdE b4646 0.45 +1.7
3,403,197 - -1.3
3,403,370 + +2.2
3,403,372 + -1.3
3,403,372 + +0.8
3,403,374 + -0.1

Or see this region's nucleotide sequence