Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrsA and ileT are separated by 68 nucleotides ileT and alaT are separated by 42 nucleotides alaT and rrlA are separated by 183 nucleotides rrlA and rrfA are separated by 93 nucleotides rrfA and mobB are separated by 269 nucleotides
b3851: rrsA - 16S ribosomal RNA (NCBI), at 4,033,554 to 4,035,095
rrsA
b3852: ileT - tRNA-Ile (NCBI), at 4,035,164 to 4,035,240
ileT
b3853: alaT - tRNA-Ala (NCBI), at 4,035,283 to 4,035,358
alaT
b3854: rrlA - 23S ribosomal RNA (NCBI), at 4,035,542 to 4,038,446
rrlA
b3855: rrfA - 5S ribosomal RNA (NCBI), at 4,038,540 to 4,038,659
rrfA
b3856: mobB - molybdopterin-guanine dinucleotide biosynthesis protein B (RefSeq), at 4,038,929 to 4,039,456
mobB
b3857: mobA - molybdopterin-guanine dinucleotide biosynthesis protein A (NCBI), at 4,039,438 to 4,040,022
mobA
Position (kb)
4035
4036
4037
4038
4039 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4038.917 kb on + strand at 4038.982 kb on - strand, within mobB at 4039.000 kb on - strand, within mobB at 4039.014 kb on - strand, within mobB at 4039.036 kb on + strand, within mobB at 4039.104 kb on + strand, within mobB at 4039.104 kb on + strand, within mobB at 4039.150 kb on - strand, within mobB at 4039.192 kb on - strand, within mobB at 4039.207 kb on - strand, within mobB at 4039.226 kb on + strand, within mobB at 4039.271 kb on - strand, within mobB at 4039.272 kb on + strand, within mobB at 4039.279 kb on + strand, within mobB at 4039.282 kb on - strand, within mobB at 4039.318 kb on - strand, within mobB at 4039.318 kb on - strand, within mobB at 4039.347 kb on - strand, within mobB at 4039.414 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 4,038,917 + -1.5 4,038,982 - mobB b3856 0.10 -0.6 4,039,000 - mobB b3856 0.13 -0.3 4,039,014 - mobB b3856 0.16 -2.8 4,039,036 + mobB b3856 0.20 -0.1 4,039,104 + mobB b3856 0.33 -1.0 4,039,104 + mobB b3856 0.33 -0.5 4,039,150 - mobB b3856 0.42 -0.1 4,039,192 - mobB b3856 0.50 +0.3 4,039,207 - mobB b3856 0.53 -2.5 4,039,226 + mobB b3856 0.56 +0.2 4,039,271 - mobB b3856 0.65 -0.2 4,039,272 + mobB b3856 0.65 -0.0 4,039,279 + mobB b3856 0.66 +0.4 4,039,282 - mobB b3856 0.67 -0.5 4,039,318 - mobB b3856 0.74 -0.4 4,039,318 - mobB b3856 0.74 -0.4 4,039,347 - mobB b3856 0.79 +0.8 4,039,414 + +0.7
Or see this region's nucleotide sequence