Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fbp and mpl are separated by 175 nucleotides mpl and yjgA are separated by 155 nucleotides yjgA and pmbA are separated by 93 nucleotides
b4232: fbp - fructose-1,6-bisphosphatase (NCBI), at 4,452,634 to 4,453,632
fbp
b4233: mpl - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (NCBI), at 4,453,808 to 4,455,181
mpl
b4234: yjgA - hypothetical protein (NCBI), at 4,455,337 to 4,455,888
yjgA
b4235: pmbA - predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 (NCBI), at 4,455,982 to 4,457,334
pmbA
Position (kb)
4453
4454
4455
4456 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4452.867 kb on - strand, within fbp at 4452.869 kb on - strand, within fbp at 4452.869 kb on - strand, within fbp at 4452.906 kb on + strand, within fbp at 4452.926 kb on + strand, within fbp at 4452.934 kb on - strand, within fbp at 4452.971 kb on + strand, within fbp at 4452.989 kb on + strand, within fbp at 4452.989 kb on + strand, within fbp at 4453.022 kb on - strand, within fbp at 4453.102 kb on + strand, within fbp at 4453.105 kb on - strand, within fbp at 4453.132 kb on - strand, within fbp at 4453.139 kb on + strand, within fbp at 4453.147 kb on + strand, within fbp at 4453.147 kb on + strand, within fbp at 4453.147 kb on + strand, within fbp at 4453.155 kb on - strand, within fbp at 4453.155 kb on - strand, within fbp at 4453.226 kb on + strand, within fbp at 4453.289 kb on + strand, within fbp at 4453.289 kb on + strand, within fbp at 4453.289 kb on + strand, within fbp at 4453.306 kb on + strand, within fbp at 4453.314 kb on - strand, within fbp at 4453.368 kb on - strand, within fbp at 4453.368 kb on - strand, within fbp at 4453.368 kb on - strand, within fbp at 4453.374 kb on - strand, within fbp at 4453.380 kb on - strand, within fbp at 4453.380 kb on - strand, within fbp at 4453.391 kb on - strand, within fbp at 4453.456 kb on + strand, within fbp at 4453.456 kb on + strand, within fbp at 4453.508 kb on - strand, within fbp at 4453.518 kb on - strand, within fbp at 4453.532 kb on - strand, within fbp at 4453.562 kb on + strand at 4453.569 kb on + strand at 4453.580 kb on - strand at 4453.580 kb on - strand at 4453.892 kb on - strand at 4454.033 kb on - strand, within mpl at 4454.066 kb on + strand, within mpl at 4454.066 kb on + strand, within mpl at 4454.072 kb on - strand, within mpl at 4454.082 kb on - strand, within mpl at 4454.082 kb on - strand, within mpl at 4454.086 kb on - strand, within mpl at 4454.087 kb on - strand, within mpl at 4454.087 kb on - strand, within mpl at 4454.112 kb on + strand, within mpl at 4454.120 kb on + strand, within mpl at 4454.141 kb on - strand, within mpl at 4454.141 kb on - strand, within mpl at 4454.141 kb on - strand, within mpl at 4454.143 kb on + strand, within mpl at 4454.178 kb on - strand, within mpl at 4454.186 kb on - strand, within mpl at 4454.288 kb on + strand, within mpl at 4454.296 kb on - strand, within mpl at 4454.296 kb on - strand, within mpl at 4454.317 kb on + strand, within mpl at 4454.317 kb on + strand, within mpl at 4454.348 kb on - strand, within mpl at 4454.385 kb on + strand, within mpl at 4454.506 kb on + strand, within mpl at 4454.508 kb on + strand, within mpl at 4454.508 kb on + strand, within mpl at 4454.624 kb on - strand, within mpl at 4454.624 kb on - strand, within mpl at 4454.697 kb on + strand, within mpl at 4454.783 kb on + strand, within mpl at 4454.896 kb on - strand, within mpl at 4454.932 kb on + strand, within mpl at 4454.944 kb on - strand, within mpl at 4454.944 kb on - strand, within mpl at 4454.968 kb on - strand, within mpl at 4455.002 kb on - strand, within mpl at 4455.020 kb on - strand, within mpl at 4455.031 kb on - strand, within mpl at 4455.047 kb on - strand at 4455.067 kb on + strand at 4455.067 kb on + strand at 4455.093 kb on + strand at 4455.129 kb on + strand at 4455.262 kb on - strand at 4455.351 kb on + strand at 4455.469 kb on - strand, within yjgA at 4455.483 kb on - strand, within yjgA at 4455.582 kb on - strand, within yjgA at 4455.628 kb on - strand, within yjgA at 4455.628 kb on - strand, within yjgA at 4455.695 kb on - strand, within yjgA at 4455.727 kb on - strand, within yjgA at 4455.924 kb on + strand at 4455.960 kb on - strand at 4455.971 kb on + strand at 4456.031 kb on - strand at 4456.031 kb on - strand at 4456.053 kb on - strand at 4456.053 kb on - strand at 4456.054 kb on + strand at 4456.062 kb on - strand at 4456.062 kb on - strand at 4456.083 kb on - strand at 4456.093 kb on - strand at 4456.131 kb on + strand, within pmbA at 4456.132 kb on + strand, within pmbA at 4456.132 kb on + strand, within pmbA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 4,452,867 - fbp b4232 0.23 -1.5 4,452,869 - fbp b4232 0.24 -0.6 4,452,869 - fbp b4232 0.24 -1.6 4,452,906 + fbp b4232 0.27 -1.3 4,452,926 + fbp b4232 0.29 +0.1 4,452,934 - fbp b4232 0.30 -0.7 4,452,971 + fbp b4232 0.34 -0.5 4,452,989 + fbp b4232 0.36 +0.5 4,452,989 + fbp b4232 0.36 -0.3 4,453,022 - fbp b4232 0.39 -0.7 4,453,102 + fbp b4232 0.47 -1.3 4,453,105 - fbp b4232 0.47 -0.8 4,453,132 - fbp b4232 0.50 -1.0 4,453,139 + fbp b4232 0.51 +3.2 4,453,147 + fbp b4232 0.51 -1.0 4,453,147 + fbp b4232 0.51 -1.1 4,453,147 + fbp b4232 0.51 -1.9 4,453,155 - fbp b4232 0.52 -2.4 4,453,155 - fbp b4232 0.52 -3.0 4,453,226 + fbp b4232 0.59 +0.1 4,453,289 + fbp b4232 0.66 -1.8 4,453,289 + fbp b4232 0.66 -1.3 4,453,289 + fbp b4232 0.66 -0.1 4,453,306 + fbp b4232 0.67 -1.3 4,453,314 - fbp b4232 0.68 -2.3 4,453,368 - fbp b4232 0.73 -1.0 4,453,368 - fbp b4232 0.73 -3.0 4,453,368 - fbp b4232 0.73 -1.7 4,453,374 - fbp b4232 0.74 -1.8 4,453,380 - fbp b4232 0.75 -0.3 4,453,380 - fbp b4232 0.75 -1.1 4,453,391 - fbp b4232 0.76 -2.0 4,453,456 + fbp b4232 0.82 -0.7 4,453,456 + fbp b4232 0.82 -1.9 4,453,508 - fbp b4232 0.87 +0.2 4,453,518 - fbp b4232 0.88 +0.3 4,453,532 - fbp b4232 0.90 -0.5 4,453,562 + -0.3 4,453,569 + +0.2 4,453,580 - +0.2 4,453,580 - -1.0 4,453,892 - -2.2 4,454,033 - mpl b4233 0.16 -2.6 4,454,066 + mpl b4233 0.19 -0.3 4,454,066 + mpl b4233 0.19 -2.2 4,454,072 - mpl b4233 0.19 +0.6 4,454,082 - mpl b4233 0.20 -2.9 4,454,082 - mpl b4233 0.20 -2.0 4,454,086 - mpl b4233 0.20 -2.8 4,454,087 - mpl b4233 0.20 -1.0 4,454,087 - mpl b4233 0.20 -2.4 4,454,112 + mpl b4233 0.22 -1.3 4,454,120 + mpl b4233 0.23 -2.1 4,454,141 - mpl b4233 0.24 -2.7 4,454,141 - mpl b4233 0.24 -3.3 4,454,141 - mpl b4233 0.24 -3.0 4,454,143 + mpl b4233 0.24 -1.2 4,454,178 - mpl b4233 0.27 -1.0 4,454,186 - mpl b4233 0.28 -1.2 4,454,288 + mpl b4233 0.35 -0.7 4,454,296 - mpl b4233 0.36 -1.3 4,454,296 - mpl b4233 0.36 -0.3 4,454,317 + mpl b4233 0.37 -2.3 4,454,317 + mpl b4233 0.37 -1.5 4,454,348 - mpl b4233 0.39 -2.9 4,454,385 + mpl b4233 0.42 -0.9 4,454,506 + mpl b4233 0.51 -2.4 4,454,508 + mpl b4233 0.51 -2.7 4,454,508 + mpl b4233 0.51 -2.6 4,454,624 - mpl b4233 0.59 -2.1 4,454,624 - mpl b4233 0.59 -2.2 4,454,697 + mpl b4233 0.65 +0.2 4,454,783 + mpl b4233 0.71 -2.2 4,454,896 - mpl b4233 0.79 -3.2 4,454,932 + mpl b4233 0.82 +0.5 4,454,944 - mpl b4233 0.83 -3.6 4,454,944 - mpl b4233 0.83 -1.7 4,454,968 - mpl b4233 0.84 -2.6 4,455,002 - mpl b4233 0.87 -1.2 4,455,020 - mpl b4233 0.88 -1.4 4,455,031 - mpl b4233 0.89 -1.9 4,455,047 - -2.3 4,455,067 + -2.8 4,455,067 + -1.6 4,455,093 + -1.6 4,455,129 + -3.2 4,455,262 - -0.4 4,455,351 + -0.5 4,455,469 - yjgA b4234 0.24 +0.7 4,455,483 - yjgA b4234 0.26 +0.5 4,455,582 - yjgA b4234 0.44 -0.4 4,455,628 - yjgA b4234 0.53 -1.5 4,455,628 - yjgA b4234 0.53 +1.0 4,455,695 - yjgA b4234 0.65 +0.5 4,455,727 - yjgA b4234 0.71 +0.4 4,455,924 + +1.1 4,455,960 - +0.2 4,455,971 + -1.0 4,456,031 - +0.3 4,456,031 - -0.7 4,456,053 - +0.1 4,456,053 - -1.3 4,456,054 + -1.3 4,456,062 - -0.5 4,456,062 - +0.1 4,456,083 - -0.2 4,456,093 - -2.5 4,456,131 + pmbA b4235 0.11 +0.6 4,456,132 + pmbA b4235 0.11 -0.3 4,456,132 + pmbA b4235 0.11 -0.4
Or see this region's nucleotide sequence