Strain Fitness in Escherichia coli BW25113 around b4221

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Cephalothin 0.0000078125 mM
4,441,144	-	ytfM	b4220	0.43	-1.2
4,441,234	-	ytfM	b4220	0.48	-2.5
4,441,234	-	ytfM	b4220	0.48	-1.1
4,441,292	+	ytfM	b4220	0.51	-1.4
4,441,306	+	ytfM	b4220	0.52	-2.3
4,441,382	+	ytfM	b4220	0.56	-1.7
4,441,406	+	ytfM	b4220	0.58	-1.5
4,441,406	+	ytfM	b4220	0.58	-2.4
4,441,416	+	ytfM	b4220	0.58	-2.5
4,441,424	-	ytfM	b4220	0.59	-3.9
4,441,441	+	ytfM	b4220	0.60	-1.9
4,441,441	+	ytfM	b4220	0.60	-1.3
4,441,442	+	ytfM	b4220	0.60	-3.2
4,441,456	+	ytfM	b4220	0.61	-1.6
4,441,458	+	ytfM	b4220	0.61	-3.5
4,441,495	+	ytfM	b4220	0.63	-0.4
4,441,503	-	ytfM	b4220	0.63	-2.3
4,441,521	-	ytfM	b4220	0.64	-1.7
4,441,559	+	ytfM	b4220	0.67	-3.5
4,441,564	+	ytfM	b4220	0.67	-2.4
4,441,564	+	ytfM	b4220	0.67	-2.2
4,441,567	-	ytfM	b4220	0.67	-1.5
4,441,614	+	ytfM	b4220	0.70	-1.8
4,441,614	+	ytfM	b4220	0.70	-3.5
4,441,639	+	ytfM	b4220	0.71	-2.0
4,441,639	+	ytfM	b4220	0.71	-0.7
4,441,653	+	ytfM	b4220	0.72	-2.5
4,441,657	-	ytfM	b4220	0.72	-2.3
4,441,657	-	ytfM	b4220	0.72	-0.9
4,441,657	-	ytfM	b4220	0.72	-1.3
4,441,717	+	ytfM	b4220	0.76	-3.5
4,441,727	-	ytfM	b4220	0.76	-2.6
4,441,828	+	ytfM	b4220	0.82	-3.7
4,441,933	-	ytfM	b4220	0.88	-1.1
4,441,933	-	ytfM	b4220	0.88	-2.8
4,441,969	-				-1.9
4,441,969	-				-2.8
4,442,033	-				-2.0
4,442,108	+				-1.3
4,442,183	+				-0.2
4,442,201	-				-1.5
4,442,225	+				-1.0
4,442,262	+				-1.1
4,442,262	+				+0.8
4,442,264	-				-1.7
4,442,303	+				-3.7
4,442,303	+				-1.3
4,442,312	-				-2.6
4,442,400	+				-1.9
4,442,448	-				-1.8
4,442,448	-				-2.3
4,442,469	+				-2.1
4,442,469	+				-2.9
4,442,512	-				-0.6
4,442,515	+	ytfN	b4221	0.10	-3.9
4,442,535	+	ytfN	b4221	0.11	-1.6
4,442,535	+	ytfN	b4221	0.11	-4.1
4,442,542	-	ytfN	b4221	0.11	-2.9
4,442,543	-	ytfN	b4221	0.11	-3.4
4,442,544	+	ytfN	b4221	0.11	-2.4
4,442,544	+	ytfN	b4221	0.11	-2.9
4,442,549	+	ytfN	b4221	0.11	+0.2
4,442,557	-	ytfN	b4221	0.11	-2.8
4,442,557	-	ytfN	b4221	0.11	-2.0
4,442,557	-	ytfN	b4221	0.11	-1.5
4,442,571	+	ytfN	b4221	0.12	-0.8
4,442,571	+	ytfN	b4221	0.12	-2.6
4,442,594	-	ytfN	b4221	0.12	-1.2
4,442,634	+	ytfN	b4221	0.13	-3.1
4,442,634	+	ytfN	b4221	0.13	-1.9
4,442,704	+	ytfN	b4221	0.15	-2.3
4,442,704	+	ytfN	b4221	0.15	+0.3
4,442,818	-	ytfN	b4221	0.18	-1.9
4,442,853	-	ytfN	b4221	0.19	-2.3
4,442,853	-	ytfN	b4221	0.19	-3.4
4,442,855	+	ytfN	b4221	0.19	-0.4
4,442,872	+	ytfN	b4221	0.19	-0.7
4,442,890	+	ytfN	b4221	0.20	-1.5
4,442,890	+	ytfN	b4221	0.20	-0.0
4,442,912	-	ytfN	b4221	0.21	-2.9
4,443,017	+	ytfN	b4221	0.23	+1.9
4,443,017	+	ytfN	b4221	0.23	-2.3
4,443,031	+	ytfN	b4221	0.24	-2.4
4,443,033	+	ytfN	b4221	0.24	-3.1
4,443,041	-	ytfN	b4221	0.24	-3.1
4,443,041	-	ytfN	b4221	0.24	-3.5
4,443,041	-	ytfN	b4221	0.24	-1.8
4,443,041	-	ytfN	b4221	0.24	-2.8
4,443,053	-	ytfN	b4221	0.24	-1.5
4,443,053	-	ytfN	b4221	0.24	+0.3
4,443,106	+	ytfN	b4221	0.26	-1.3
4,443,160	+	ytfN	b4221	0.27	+0.0
4,443,212	+	ytfN	b4221	0.28	-2.2
4,443,212	+	ytfN	b4221	0.28	-1.9
4,443,212	+	ytfN	b4221	0.28	-1.0
4,443,233	+	ytfN	b4221	0.29	-1.3
4,443,244	+	ytfN	b4221	0.29	-2.2
4,443,244	+	ytfN	b4221	0.29	-3.3
4,443,244	+	ytfN	b4221	0.29	-2.8
4,443,250	+	ytfN	b4221	0.29	-4.5
4,443,252	-	ytfN	b4221	0.30	-1.9
4,443,266	-	ytfN	b4221	0.30	+1.6
4,443,309	-	ytfN	b4221	0.31	-1.7
4,443,309	-	ytfN	b4221	0.31	-4.2
4,443,309	-	ytfN	b4221	0.31	-2.3
4,443,314	+	ytfN	b4221	0.31	-2.2
4,443,339	-	ytfN	b4221	0.32	-2.5
4,443,339	-	ytfN	b4221	0.32	-4.8
4,443,447	-	ytfN	b4221	0.35	-2.9
4,443,447	-	ytfN	b4221	0.35	-0.3
4,443,452	-	ytfN	b4221	0.35	-2.4
4,443,565	-	ytfN	b4221	0.38	-3.3
4,443,581	-	ytfN	b4221	0.38	-1.3
4,443,698	-	ytfN	b4221	0.41	-2.4
4,443,719	-	ytfN	b4221	0.42	-2.3
4,443,789	-	ytfN	b4221	0.44	-3.5
4,443,793	+	ytfN	b4221	0.44	+0.1
4,443,793	+	ytfN	b4221	0.44	-1.6
4,443,818	+	ytfN	b4221	0.45	-2.3
4,443,820	-	ytfN	b4221	0.45	-3.2
4,443,840	+	ytfN	b4221	0.45	-3.3
4,444,001	+	ytfN	b4221	0.49	-0.2
4,444,015	-	ytfN	b4221	0.50	-1.0
4,444,015	-	ytfN	b4221	0.50	-1.3
4,444,057	-	ytfN	b4221	0.51	-1.9
4,444,057	-	ytfN	b4221	0.51	-2.7
4,444,058	-	ytfN	b4221	0.51	-1.4
4,444,090	+	ytfN	b4221	0.52	-2.2
4,444,090	+	ytfN	b4221	0.52	-2.3
4,444,121	+	ytfN	b4221	0.53	-2.4
4,444,121	+	ytfN	b4221	0.53	-2.0
4,444,194	-	ytfN	b4221	0.54	-1.5
4,444,194	-	ytfN	b4221	0.54	-2.3
4,444,246	+	ytfN	b4221	0.56	-3.7
4,444,246	-	ytfN	b4221	0.56	-2.0
4,444,263	+	ytfN	b4221	0.56	-2.9
4,444,263	+	ytfN	b4221	0.56	-1.5
4,444,349	-	ytfN	b4221	0.59	-2.2
4,444,349	-	ytfN	b4221	0.59	-0.7
4,444,386	-	ytfN	b4221	0.60	-2.0
4,444,386	-	ytfN	b4221	0.60	-0.7
4,444,391	-	ytfN	b4221	0.60	-2.3
4,444,412	+	ytfN	b4221	0.60	-0.2
4,444,420	-	ytfN	b4221	0.60	-2.8
4,444,444	+	ytfN	b4221	0.61	-2.0
4,444,444	+	ytfN	b4221	0.61	-3.2
4,444,510	-	ytfN	b4221	0.63	-1.3
4,444,570	-	ytfN	b4221	0.64	-2.4
4,444,602	+	ytfN	b4221	0.65	-2.3
4,444,602	+	ytfN	b4221	0.65	-2.0
4,444,602	+	ytfN	b4221	0.65	+0.1
4,444,612	+	ytfN	b4221	0.66	-2.7
4,444,612	+	ytfN	b4221	0.66	-0.1
4,444,626	-	ytfN	b4221	0.66	-3.0
4,444,638	+	ytfN	b4221	0.66	-4.5
4,444,653	+	ytfN	b4221	0.67	-1.7
4,444,653	+	ytfN	b4221	0.67	-2.1
4,444,700	-	ytfN	b4221	0.68	-3.7
4,444,800	-	ytfN	b4221	0.71	-3.3
4,444,895	+	ytfN	b4221	0.73	-2.9
4,444,895	+	ytfN	b4221	0.73	+0.7
4,444,898	-	ytfN	b4221	0.73	-2.6
4,444,898	-	ytfN	b4221	0.73	-2.0
4,444,940	-	ytfN	b4221	0.74	-2.5
4,444,991	+	ytfN	b4221	0.76	-2.0
4,444,991	+	ytfN	b4221	0.76	-2.2
4,445,044	+	ytfN	b4221	0.77	-3.0
4,445,044	+	ytfN	b4221	0.77	-2.3
4,445,063	-	ytfN	b4221	0.77	-3.9
4,445,063	-	ytfN	b4221	0.77	+1.3
4,445,065	+	ytfN	b4221	0.78	-0.3
4,445,077	-	ytfN	b4221	0.78	-2.8
4,445,079	+	ytfN	b4221	0.78	-1.0
4,445,081	+	ytfN	b4221	0.78	-3.5
4,445,118	+	ytfN	b4221	0.79	-0.7
4,445,118	+	ytfN	b4221	0.79	+1.6
4,445,141	-	ytfN	b4221	0.80	-3.6
4,445,154	+	ytfN	b4221	0.80	-2.5
4,445,154	+	ytfN	b4221	0.80	-1.9
4,445,237	+	ytfN	b4221	0.82	-1.7
4,445,252	-	ytfN	b4221	0.82	-1.1
4,445,273	-	ytfN	b4221	0.83	-3.9
4,445,320	+	ytfN	b4221	0.84	-2.4
4,445,535	-	ytfN	b4221	0.90	-2.3
4,445,639	+				-2.2
4,445,668	-				-0.7
4,445,668	-				-2.8
4,445,736	+				-0.2
4,445,737	+				-0.3
4,445,737	+				-1.7
4,445,770	-				-2.2
4,445,794	+				-0.6
4,445,806	+				-0.2
4,445,806	+				-0.9
4,445,806	+				-2.5
4,445,836	+				+1.4
4,445,836	+				-0.7
4,445,859	-				-2.3
4,445,866	+				+0.4
4,445,866	+				-2.6
4,445,962	+	ytfP	b4222	0.13	-2.0
4,445,962	+	ytfP	b4222	0.13	+0.8
4,446,046	-	ytfP	b4222	0.38	-1.7
4,446,131	+	ytfP	b4222	0.63	-1.0
4,446,166	+	ytfP	b4222	0.73	-1.0
4,446,166	+	ytfP	b4222	0.73	-1.5
4,446,166	+	ytfP	b4222	0.73	+1.7
4,446,176	+	ytfP	b4222	0.76	-0.4
4,446,176	-	ytfP	b4222	0.76	-1.0
4,446,176	-	ytfP	b4222	0.76	+0.7
4,446,176	-	ytfP	b4222	0.76	+1.6
4,446,184	-	ytfP	b4222	0.78	-0.9
4,446,184	-	ytfP	b4222	0.78	-0.7
4,446,240	+				-1.5
4,446,259	+				+0.8
4,446,260	+				-1.5
4,446,272	-				-0.3
4,446,273	-				+1.3
4,446,273	-				-0.3
4,446,375	-				-0.8
4,446,454	+				-1.5
4,446,454	+				-1.9
4,446,496	+	chpS	b4224	0.10	-0.8
4,446,501	+	chpS	b4224	0.12	-1.7
4,446,501	+	chpS	b4224	0.12	-1.0
4,446,509	-	chpS	b4224	0.15	+0.3
4,446,509	-	chpS	b4224	0.15	-1.5
4,446,514	-	chpS	b4224	0.17	-1.0
4,446,559	+	chpS	b4224	0.35	+2.7
4,446,569	-	chpS	b4224	0.39	+1.7
4,446,569	-	chpS	b4224	0.39	-1.0
4,446,646	-	chpS	b4224	0.70	+1.0
4,446,652	-	chpS	b4224	0.72	+1.9
4,446,746	+				-0.4
4,446,777	+	chpB	b4225	0.18	-0.4
4,446,787	+	chpB	b4225	0.21	+0.2
4,446,787	+	chpB	b4225	0.21	-0.4
4,446,787	-	chpB	b4225	0.21	+1.9
4,446,787	-	chpB	b4225	0.21	-1.7
4,446,794	+	chpB	b4225	0.23	-0.2
4,446,910	+	chpB	b4225	0.56	-2.0