Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sugE and blc overlap by 4 nucleotides blc and ampC are separated by 88 nucleotides ampC and frdD are separated by 62 nucleotides frdD and frdC are separated by 10 nucleotides frdC and frdB are separated by 10 nucleotides
b4148: sugE - suppresses groEL, may be chaperone (VIMSS), at 4,374,898 to 4,375,215
sugE
b4149: blc - outer membrane lipoprotein (lipocalin) (NCBI), at 4,375,212 to 4,375,745
blc
b4150: ampC - beta-lactamase/D-alanine carboxypeptidase (NCBI), at 4,375,834 to 4,376,967
ampC
b4151: frdD - fumarate reductase subunit D (NCBI), at 4,377,030 to 4,377,389
frdD
b4152: frdC - fumarate reductase subunit C (NCBI), at 4,377,400 to 4,377,795
frdC
b4153: frdB - fumarate reductase (anaerobic), Fe-S subunit (NCBI), at 4,377,806 to 4,378,540
frdB
Position (kb)
4375
4376
4377 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 4374.869 kb on - strand at 4374.869 kb on - strand at 4374.900 kb on + strand at 4374.900 kb on + strand at 4374.908 kb on - strand at 4374.909 kb on - strand at 4374.909 kb on - strand at 4374.938 kb on - strand, within sugE at 4374.939 kb on + strand, within sugE at 4374.948 kb on - strand, within sugE at 4375.022 kb on - strand, within sugE at 4375.030 kb on + strand, within sugE at 4375.072 kb on - strand, within sugE at 4375.073 kb on + strand, within sugE at 4375.118 kb on + strand, within sugE at 4375.210 kb on + strand at 4375.210 kb on + strand at 4375.216 kb on + strand at 4375.218 kb on - strand at 4375.218 kb on - strand at 4375.385 kb on + strand, within blc at 4375.402 kb on + strand, within blc at 4375.428 kb on - strand, within blc at 4375.428 kb on - strand, within blc at 4375.464 kb on - strand, within blc at 4375.481 kb on - strand, within blc at 4375.490 kb on - strand, within blc at 4375.514 kb on - strand, within blc at 4375.514 kb on - strand, within blc at 4375.550 kb on + strand, within blc at 4375.550 kb on + strand, within blc at 4375.554 kb on - strand, within blc at 4375.742 kb on - strand at 4375.742 kb on - strand at 4375.774 kb on + strand at 4375.782 kb on - strand at 4375.784 kb on + strand at 4375.983 kb on + strand, within ampC at 4375.998 kb on + strand, within ampC at 4375.998 kb on + strand, within ampC at 4376.008 kb on - strand, within ampC at 4376.110 kb on + strand, within ampC at 4376.323 kb on - strand, within ampC at 4376.400 kb on - strand, within ampC at 4376.427 kb on + strand, within ampC at 4376.427 kb on + strand, within ampC at 4376.484 kb on + strand at 4376.493 kb on + strand, within ampC at 4376.541 kb on + strand, within ampC at 4376.541 kb on + strand, within ampC at 4376.581 kb on + strand, within ampC at 4376.594 kb on + strand, within ampC at 4376.596 kb on + strand, within ampC at 4376.634 kb on + strand, within ampC at 4376.642 kb on - strand, within ampC at 4376.690 kb on - strand, within ampC at 4376.690 kb on - strand, within ampC at 4376.693 kb on - strand, within ampC at 4376.794 kb on + strand, within ampC at 4376.794 kb on + strand, within ampC at 4376.800 kb on + strand, within ampC at 4376.982 kb on + strand at 4377.019 kb on - strand at 4377.043 kb on + strand at 4377.043 kb on + strand at 4377.078 kb on - strand, within frdD at 4377.078 kb on - strand, within frdD at 4377.126 kb on + strand, within frdD at 4377.126 kb on + strand, within frdD at 4377.155 kb on - strand, within frdD at 4377.155 kb on - strand, within frdD at 4377.320 kb on + strand, within frdD at 4377.320 kb on + strand, within frdD at 4377.401 kb on + strand at 4377.401 kb on + strand at 4377.401 kb on + strand at 4377.404 kb on - strand at 4377.404 kb on - strand at 4377.406 kb on - strand at 4377.409 kb on - strand at 4377.409 kb on - strand at 4377.409 kb on - strand at 4377.446 kb on - strand, within frdC at 4377.446 kb on - strand, within frdC at 4377.550 kb on + strand, within frdC at 4377.576 kb on + strand, within frdC at 4377.777 kb on + strand at 4377.794 kb on + strand at 4377.794 kb on + strand at 4377.812 kb on - strand at 4377.812 kb on - strand at 4377.812 kb on - strand at 4377.889 kb on + strand, within frdB at 4377.889 kb on - strand, within frdB at 4377.909 kb on - strand, within frdB at 4377.909 kb on - strand, within frdB at 4377.919 kb on + strand, within frdB at 4377.939 kb on - strand, within frdB at 4377.966 kb on - strand, within frdB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 4,374,869 - +0.1 4,374,869 - -1.5 4,374,900 + +0.4 4,374,900 + -0.9 4,374,908 - +1.4 4,374,909 - -0.6 4,374,909 - +0.6 4,374,938 - sugE b4148 0.13 +1.0 4,374,939 + sugE b4148 0.13 -1.0 4,374,948 - sugE b4148 0.16 +1.8 4,375,022 - sugE b4148 0.39 +1.7 4,375,030 + sugE b4148 0.42 -1.0 4,375,072 - sugE b4148 0.55 +0.1 4,375,073 + sugE b4148 0.55 -3.7 4,375,118 + sugE b4148 0.69 -0.3 4,375,210 + -0.2 4,375,210 + -0.2 4,375,216 + -0.8 4,375,218 - +0.6 4,375,218 - +0.5 4,375,385 + blc b4149 0.32 -1.1 4,375,402 + blc b4149 0.36 +0.5 4,375,428 - blc b4149 0.40 -0.8 4,375,428 - blc b4149 0.40 +1.4 4,375,464 - blc b4149 0.47 -1.3 4,375,481 - blc b4149 0.50 -2.7 4,375,490 - blc b4149 0.52 +1.2 4,375,514 - blc b4149 0.57 +0.1 4,375,514 - blc b4149 0.57 +1.0 4,375,550 + blc b4149 0.63 -3.3 4,375,550 + blc b4149 0.63 -3.3 4,375,554 - blc b4149 0.64 -0.3 4,375,742 - +0.4 4,375,742 - +1.2 4,375,774 + -2.9 4,375,782 - -0.9 4,375,784 + -0.7 4,375,983 + ampC b4150 0.13 -1.6 4,375,998 + ampC b4150 0.14 -3.6 4,375,998 + ampC b4150 0.14 -0.6 4,376,008 - ampC b4150 0.15 -2.6 4,376,110 + ampC b4150 0.24 -3.8 4,376,323 - ampC b4150 0.43 -2.9 4,376,400 - ampC b4150 0.50 -1.8 4,376,427 + ampC b4150 0.52 -2.5 4,376,427 + ampC b4150 0.52 -2.1 4,376,484 + +0.4 4,376,493 + ampC b4150 0.58 -1.6 4,376,541 + ampC b4150 0.62 -3.7 4,376,541 + ampC b4150 0.62 -3.1 4,376,581 + ampC b4150 0.66 -4.0 4,376,594 + ampC b4150 0.67 -3.7 4,376,596 + ampC b4150 0.67 -4.8 4,376,634 + ampC b4150 0.71 -3.4 4,376,642 - ampC b4150 0.71 -5.0 4,376,690 - ampC b4150 0.75 -2.2 4,376,690 - ampC b4150 0.75 -2.3 4,376,693 - ampC b4150 0.76 -4.2 4,376,794 + ampC b4150 0.85 -1.9 4,376,794 + ampC b4150 0.85 -2.3 4,376,800 + ampC b4150 0.85 -4.1 4,376,982 + -2.9 4,377,019 - +5.0 4,377,043 + -2.3 4,377,043 + -2.4 4,377,078 - frdD b4151 0.13 -1.4 4,377,078 - frdD b4151 0.13 +4.5 4,377,126 + frdD b4151 0.27 +0.4 4,377,126 + frdD b4151 0.27 +0.4 4,377,155 - frdD b4151 0.35 -0.5 4,377,155 - frdD b4151 0.35 +1.3 4,377,320 + frdD b4151 0.81 +0.4 4,377,320 + frdD b4151 0.81 -3.0 4,377,401 + +0.2 4,377,401 + -2.2 4,377,401 + -0.3 4,377,404 - -0.6 4,377,404 - +2.9 4,377,406 - -0.3 4,377,409 - +1.5 4,377,409 - +3.4 4,377,409 - +3.7 4,377,446 - frdC b4152 0.12 +1.7 4,377,446 - frdC b4152 0.12 +5.2 4,377,550 + frdC b4152 0.38 -1.2 4,377,576 + frdC b4152 0.44 -1.9 4,377,777 + -1.2 4,377,794 + -1.8 4,377,794 + -0.9 4,377,812 - +0.6 4,377,812 - +2.0 4,377,812 - +1.4 4,377,889 + frdB b4153 0.11 -0.0 4,377,889 - frdB b4153 0.11 -1.6 4,377,909 - frdB b4153 0.14 +1.7 4,377,909 - frdB b4153 0.14 -0.6 4,377,919 + frdB b4153 0.15 -2.2 4,377,939 - frdB b4153 0.18 +2.5 4,377,966 - frdB b4153 0.22 +3.6
Or see this region's nucleotide sequence