Strain Fitness in Escherichia coli BW25113 around b4150

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsugE and blc overlap by 4 nucleotidesblc and ampC are separated by 88 nucleotidesampC and frdD are separated by 62 nucleotidesfrdD and frdC are separated by 10 nucleotidesfrdC and frdB are separated by 10 nucleotides b4148: sugE - suppresses groEL, may be chaperone (VIMSS), at 4,374,898 to 4,375,215 sugE b4149: blc - outer membrane lipoprotein (lipocalin) (NCBI), at 4,375,212 to 4,375,745 blc b4150: ampC - beta-lactamase/D-alanine carboxypeptidase (NCBI), at 4,375,834 to 4,376,967 ampC b4151: frdD - fumarate reductase subunit D (NCBI), at 4,377,030 to 4,377,389 frdD b4152: frdC - fumarate reductase subunit C (NCBI), at 4,377,400 to 4,377,795 frdC b4153: frdB - fumarate reductase (anaerobic), Fe-S subunit (NCBI), at 4,377,806 to 4,378,540 frdB Position (kb) 4375 4376 4377Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4 5at 4374.869 kb on - strandat 4374.869 kb on - strandat 4374.900 kb on + strandat 4374.900 kb on + strandat 4374.908 kb on - strandat 4374.909 kb on - strandat 4374.909 kb on - strandat 4374.938 kb on - strand, within sugEat 4374.939 kb on + strand, within sugEat 4374.948 kb on - strand, within sugEat 4375.022 kb on - strand, within sugEat 4375.030 kb on + strand, within sugEat 4375.072 kb on - strand, within sugEat 4375.073 kb on + strand, within sugEat 4375.118 kb on + strand, within sugEat 4375.210 kb on + strandat 4375.210 kb on + strandat 4375.216 kb on + strandat 4375.218 kb on - strandat 4375.218 kb on - strandat 4375.385 kb on + strand, within blcat 4375.402 kb on + strand, within blcat 4375.428 kb on - strand, within blcat 4375.428 kb on - strand, within blcat 4375.464 kb on - strand, within blcat 4375.481 kb on - strand, within blcat 4375.490 kb on - strand, within blcat 4375.514 kb on - strand, within blcat 4375.514 kb on - strand, within blcat 4375.550 kb on + strand, within blcat 4375.550 kb on + strand, within blcat 4375.554 kb on - strand, within blcat 4375.742 kb on - strandat 4375.742 kb on - strandat 4375.774 kb on + strandat 4375.782 kb on - strandat 4375.784 kb on + strandat 4375.983 kb on + strand, within ampCat 4375.998 kb on + strand, within ampCat 4375.998 kb on + strand, within ampCat 4376.008 kb on - strand, within ampCat 4376.110 kb on + strand, within ampCat 4376.323 kb on - strand, within ampCat 4376.400 kb on - strand, within ampCat 4376.427 kb on + strand, within ampCat 4376.427 kb on + strand, within ampCat 4376.484 kb on + strandat 4376.493 kb on + strand, within ampCat 4376.541 kb on + strand, within ampCat 4376.541 kb on + strand, within ampCat 4376.581 kb on + strand, within ampCat 4376.594 kb on + strand, within ampCat 4376.596 kb on + strand, within ampCat 4376.634 kb on + strand, within ampCat 4376.642 kb on - strand, within ampCat 4376.690 kb on - strand, within ampCat 4376.690 kb on - strand, within ampCat 4376.693 kb on - strand, within ampCat 4376.794 kb on + strand, within ampCat 4376.794 kb on + strand, within ampCat 4376.800 kb on + strand, within ampCat 4376.982 kb on + strandat 4377.019 kb on - strandat 4377.043 kb on + strandat 4377.043 kb on + strandat 4377.078 kb on - strand, within frdDat 4377.078 kb on - strand, within frdDat 4377.126 kb on + strand, within frdDat 4377.126 kb on + strand, within frdDat 4377.155 kb on - strand, within frdDat 4377.155 kb on - strand, within frdDat 4377.320 kb on + strand, within frdDat 4377.320 kb on + strand, within frdDat 4377.401 kb on + strandat 4377.401 kb on + strandat 4377.401 kb on + strandat 4377.404 kb on - strandat 4377.404 kb on - strandat 4377.406 kb on - strandat 4377.409 kb on - strandat 4377.409 kb on - strandat 4377.409 kb on - strandat 4377.446 kb on - strand, within frdCat 4377.446 kb on - strand, within frdCat 4377.550 kb on + strand, within frdCat 4377.576 kb on + strand, within frdCat 4377.777 kb on + strandat 4377.794 kb on + strandat 4377.794 kb on + strandat 4377.812 kb on - strandat 4377.812 kb on - strandat 4377.812 kb on - strandat 4377.889 kb on + strand, within frdBat 4377.889 kb on - strand, within frdBat 4377.909 kb on - strand, within frdBat 4377.909 kb on - strand, within frdBat 4377.919 kb on + strand, within frdBat 4377.939 kb on - strand, within frdBat 4377.966 kb on - strand, within frdB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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4,374,869 - +0.1
4,374,869 - -1.5
4,374,900 + +0.4
4,374,900 + -0.9
4,374,908 - +1.4
4,374,909 - -0.6
4,374,909 - +0.6
4,374,938 - sugE b4148 0.13 +1.0
4,374,939 + sugE b4148 0.13 -1.0
4,374,948 - sugE b4148 0.16 +1.8
4,375,022 - sugE b4148 0.39 +1.7
4,375,030 + sugE b4148 0.42 -1.0
4,375,072 - sugE b4148 0.55 +0.1
4,375,073 + sugE b4148 0.55 -3.7
4,375,118 + sugE b4148 0.69 -0.3
4,375,210 + -0.2
4,375,210 + -0.2
4,375,216 + -0.8
4,375,218 - +0.6
4,375,218 - +0.5
4,375,385 + blc b4149 0.32 -1.1
4,375,402 + blc b4149 0.36 +0.5
4,375,428 - blc b4149 0.40 -0.8
4,375,428 - blc b4149 0.40 +1.4
4,375,464 - blc b4149 0.47 -1.3
4,375,481 - blc b4149 0.50 -2.7
4,375,490 - blc b4149 0.52 +1.2
4,375,514 - blc b4149 0.57 +0.1
4,375,514 - blc b4149 0.57 +1.0
4,375,550 + blc b4149 0.63 -3.3
4,375,550 + blc b4149 0.63 -3.3
4,375,554 - blc b4149 0.64 -0.3
4,375,742 - +0.4
4,375,742 - +1.2
4,375,774 + -2.9
4,375,782 - -0.9
4,375,784 + -0.7
4,375,983 + ampC b4150 0.13 -1.6
4,375,998 + ampC b4150 0.14 -3.6
4,375,998 + ampC b4150 0.14 -0.6
4,376,008 - ampC b4150 0.15 -2.6
4,376,110 + ampC b4150 0.24 -3.8
4,376,323 - ampC b4150 0.43 -2.9
4,376,400 - ampC b4150 0.50 -1.8
4,376,427 + ampC b4150 0.52 -2.5
4,376,427 + ampC b4150 0.52 -2.1
4,376,484 + +0.4
4,376,493 + ampC b4150 0.58 -1.6
4,376,541 + ampC b4150 0.62 -3.7
4,376,541 + ampC b4150 0.62 -3.1
4,376,581 + ampC b4150 0.66 -4.0
4,376,594 + ampC b4150 0.67 -3.7
4,376,596 + ampC b4150 0.67 -4.8
4,376,634 + ampC b4150 0.71 -3.4
4,376,642 - ampC b4150 0.71 -5.0
4,376,690 - ampC b4150 0.75 -2.2
4,376,690 - ampC b4150 0.75 -2.3
4,376,693 - ampC b4150 0.76 -4.2
4,376,794 + ampC b4150 0.85 -1.9
4,376,794 + ampC b4150 0.85 -2.3
4,376,800 + ampC b4150 0.85 -4.1
4,376,982 + -2.9
4,377,019 - +5.0
4,377,043 + -2.3
4,377,043 + -2.4
4,377,078 - frdD b4151 0.13 -1.4
4,377,078 - frdD b4151 0.13 +4.5
4,377,126 + frdD b4151 0.27 +0.4
4,377,126 + frdD b4151 0.27 +0.4
4,377,155 - frdD b4151 0.35 -0.5
4,377,155 - frdD b4151 0.35 +1.3
4,377,320 + frdD b4151 0.81 +0.4
4,377,320 + frdD b4151 0.81 -3.0
4,377,401 + +0.2
4,377,401 + -2.2
4,377,401 + -0.3
4,377,404 - -0.6
4,377,404 - +2.9
4,377,406 - -0.3
4,377,409 - +1.5
4,377,409 - +3.4
4,377,409 - +3.7
4,377,446 - frdC b4152 0.12 +1.7
4,377,446 - frdC b4152 0.12 +5.2
4,377,550 + frdC b4152 0.38 -1.2
4,377,576 + frdC b4152 0.44 -1.9
4,377,777 + -1.2
4,377,794 + -1.8
4,377,794 + -0.9
4,377,812 - +0.6
4,377,812 - +2.0
4,377,812 - +1.4
4,377,889 + frdB b4153 0.11 -0.0
4,377,889 - frdB b4153 0.11 -1.6
4,377,909 - frdB b4153 0.14 +1.7
4,377,909 - frdB b4153 0.14 -0.6
4,377,919 + frdB b4153 0.15 -2.2
4,377,939 - frdB b4153 0.18 +2.5
4,377,966 - frdB b4153 0.22 +3.6

Or see this region's nucleotide sequence