Strain Fitness in Escherichia coli BW25113 around b4069

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntactP and yjcH overlap by 4 nucleotidesyjcH and acs are separated by 199 nucleotidesacs and nrfA are separated by 392 nucleotides b4067: actP - acetate permease (NCBI), at 4,281,276 to 4,282,925 actP b4068: yjcH - required for glycolate transport, with actP (from data), at 4,282,922 to 4,283,236 yjcH b4069: acs - acetyl-coenzyme A synthetase (NCBI), at 4,283,436 to 4,285,394 acs b4070: nrfA - nitrite reductase, formate-dependent, cytochrome (NCBI), at 4,285,787 to 4,287,223 nrfA Position (kb) 4283 4284 4285 4286Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4282.531 kb on + strand, within actPat 4282.706 kb on - strand, within actPat 4282.718 kb on - strand, within actPat 4282.718 kb on - strand, within actPat 4282.738 kb on - strand, within actPat 4282.738 kb on - strand, within actPat 4282.740 kb on - strand, within actPat 4282.842 kb on + strandat 4282.864 kb on - strandat 4282.873 kb on - strandat 4282.909 kb on + strandat 4282.938 kb on - strandat 4282.990 kb on + strand, within yjcHat 4283.005 kb on + strand, within yjcHat 4283.013 kb on - strand, within yjcHat 4283.013 kb on - strand, within yjcHat 4283.013 kb on - strand, within yjcHat 4283.050 kb on + strand, within yjcHat 4283.121 kb on - strand, within yjcHat 4283.212 kb on - strandat 4283.212 kb on - strandat 4283.218 kb on + strandat 4283.224 kb on - strandat 4283.224 kb on - strandat 4283.291 kb on - strandat 4283.315 kb on - strandat 4283.315 kb on - strandat 4283.395 kb on + strandat 4283.395 kb on + strandat 4283.423 kb on + strandat 4283.423 kb on + strandat 4283.514 kb on + strandat 4283.514 kb on + strandat 4283.595 kb on - strandat 4283.678 kb on - strand, within acsat 4283.678 kb on - strand, within acsat 4283.712 kb on + strand, within acsat 4283.749 kb on - strand, within acsat 4283.808 kb on + strand, within acsat 4283.855 kb on + strand, within acsat 4283.855 kb on + strand, within acsat 4283.865 kb on + strand, within acsat 4283.956 kb on + strand, within acsat 4283.974 kb on - strand, within acsat 4283.981 kb on - strand, within acsat 4283.981 kb on - strand, within acsat 4283.983 kb on + strand, within acsat 4284.007 kb on - strand, within acsat 4284.032 kb on - strand, within acsat 4284.032 kb on - strand, within acsat 4284.215 kb on + strand, within acsat 4284.215 kb on + strand, within acsat 4284.229 kb on - strand, within acsat 4284.233 kb on + strand, within acsat 4284.255 kb on - strand, within acsat 4284.398 kb on - strand, within acsat 4284.446 kb on - strand, within acsat 4284.449 kb on - strand, within acsat 4284.454 kb on - strand, within acsat 4284.486 kb on + strand, within acsat 4284.521 kb on - strand, within acsat 4284.521 kb on - strand, within acsat 4284.541 kb on + strand, within acsat 4284.587 kb on - strand, within acsat 4284.675 kb on - strand, within acsat 4284.677 kb on + strandat 4284.690 kb on - strand, within acsat 4284.690 kb on - strand, within acsat 4284.792 kb on - strand, within acsat 4284.811 kb on - strand, within acsat 4284.811 kb on - strand, within acsat 4284.841 kb on + strand, within acsat 4284.983 kb on - strand, within acsat 4285.188 kb on + strand, within acsat 4285.199 kb on + strandat 4285.326 kb on - strandat 4285.326 kb on - strandat 4285.406 kb on + strandat 4285.695 kb on + strandat 4285.711 kb on + strandat 4285.896 kb on + strandat 4285.948 kb on + strand, within nrfAat 4285.948 kb on + strand, within nrfAat 4285.967 kb on + strand, within nrfAat 4286.025 kb on - strand, within nrfAat 4286.025 kb on - strand, within nrfAat 4286.035 kb on + strand, within nrfAat 4286.035 kb on - strand, within nrfAat 4286.089 kb on + strand, within nrfAat 4286.089 kb on + strand, within nrfAat 4286.107 kb on - strand, within nrfAat 4286.107 kb on - strand, within nrfAat 4286.109 kb on - strand, within nrfAat 4286.135 kb on + strand, within nrfAat 4286.135 kb on + strand, within nrfAat 4286.168 kb on - strand, within nrfAat 4286.168 kb on - strand, within nrfAat 4286.179 kb on - strand, within nrfAat 4286.340 kb on - strand, within nrfA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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4,282,531 + actP b4067 0.76 +0.2
4,282,706 - actP b4067 0.87 +0.3
4,282,718 - actP b4067 0.87 +0.7
4,282,718 - actP b4067 0.87 -2.5
4,282,738 - actP b4067 0.89 -0.4
4,282,738 - actP b4067 0.89 -0.3
4,282,740 - actP b4067 0.89 +2.4
4,282,842 + -0.7
4,282,864 - +0.8
4,282,873 - -0.6
4,282,909 + -0.5
4,282,938 - +0.9
4,282,990 + yjcH b4068 0.22 -0.3
4,283,005 + yjcH b4068 0.26 -2.2
4,283,013 - yjcH b4068 0.29 -2.1
4,283,013 - yjcH b4068 0.29 -0.8
4,283,013 - yjcH b4068 0.29 -0.2
4,283,050 + yjcH b4068 0.41 -1.9
4,283,121 - yjcH b4068 0.63 -0.9
4,283,212 - -0.2
4,283,212 - -0.5
4,283,218 + -0.0
4,283,224 - -1.8
4,283,224 - -1.6
4,283,291 - +0.0
4,283,315 - -1.0
4,283,315 - +0.3
4,283,395 + +1.6
4,283,395 + +0.1
4,283,423 + -0.3
4,283,423 + -0.8
4,283,514 + -0.2
4,283,514 + -0.4
4,283,595 - -0.6
4,283,678 - acs b4069 0.12 +2.8
4,283,678 - acs b4069 0.12 +0.4
4,283,712 + acs b4069 0.14 +0.1
4,283,749 - acs b4069 0.16 -1.5
4,283,808 + acs b4069 0.19 -0.3
4,283,855 + acs b4069 0.21 +1.2
4,283,855 + acs b4069 0.21 -0.5
4,283,865 + acs b4069 0.22 +0.0
4,283,956 + acs b4069 0.27 +0.4
4,283,974 - acs b4069 0.27 -1.9
4,283,981 - acs b4069 0.28 -0.9
4,283,981 - acs b4069 0.28 -0.2
4,283,983 + acs b4069 0.28 +0.4
4,284,007 - acs b4069 0.29 -1.9
4,284,032 - acs b4069 0.30 +1.4
4,284,032 - acs b4069 0.30 +0.6
4,284,215 + acs b4069 0.40 -0.5
4,284,215 + acs b4069 0.40 +0.1
4,284,229 - acs b4069 0.40 +0.8
4,284,233 + acs b4069 0.41 +0.4
4,284,255 - acs b4069 0.42 +0.1
4,284,398 - acs b4069 0.49 +2.2
4,284,446 - acs b4069 0.52 +0.6
4,284,449 - acs b4069 0.52 -1.6
4,284,454 - acs b4069 0.52 -1.7
4,284,486 + acs b4069 0.54 -1.2
4,284,521 - acs b4069 0.55 -0.6
4,284,521 - acs b4069 0.55 -1.1
4,284,541 + acs b4069 0.56 +0.4
4,284,587 - acs b4069 0.59 -0.2
4,284,675 - acs b4069 0.63 +0.6
4,284,677 + -0.2
4,284,690 - acs b4069 0.64 -1.6
4,284,690 - acs b4069 0.64 +0.8
4,284,792 - acs b4069 0.69 -1.8
4,284,811 - acs b4069 0.70 +2.3
4,284,811 - acs b4069 0.70 +0.5
4,284,841 + acs b4069 0.72 -1.5
4,284,983 - acs b4069 0.79 -0.8
4,285,188 + acs b4069 0.89 -2.6
4,285,199 + -0.2
4,285,326 - +1.1
4,285,326 - +1.0
4,285,406 + +1.9
4,285,695 + -1.0
4,285,711 + -0.2
4,285,896 + -0.8
4,285,948 + nrfA b4070 0.11 -0.6
4,285,948 + nrfA b4070 0.11 -2.3
4,285,967 + nrfA b4070 0.13 -1.1
4,286,025 - nrfA b4070 0.17 +1.9
4,286,025 - nrfA b4070 0.17 +1.7
4,286,035 + nrfA b4070 0.17 -0.8
4,286,035 - nrfA b4070 0.17 -1.2
4,286,089 + nrfA b4070 0.21 +0.2
4,286,089 + nrfA b4070 0.21 +0.2
4,286,107 - nrfA b4070 0.22 -1.7
4,286,107 - nrfA b4070 0.22 -1.5
4,286,109 - nrfA b4070 0.22 -0.5
4,286,135 + nrfA b4070 0.24 -1.1
4,286,135 + nrfA b4070 0.24 +0.5
4,286,168 - nrfA b4070 0.27 +0.1
4,286,168 - nrfA b4070 0.27 -1.0
4,286,179 - nrfA b4070 0.27 +1.1
4,286,340 - nrfA b4070 0.38 -0.8

Or see this region's nucleotide sequence