Strain Fitness in Escherichia coli BW25113 around b3950

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfrwC and frwB are separated by 14 nucleotidesfrwB and pflD are separated by 50 nucleotides b3949: frwC - predicted enzyme IIC component of PTS (NCBI), at 4,140,553 to 4,141,632 frwC b3950: frwB - predicted enzyme IIB component of PTS (NCBI), at 4,141,647 to 4,141,967 frwB b3951: pflD - predicted formate acetyltransferase 2 (pyruvate formate lyase II) (NCBI), at 4,142,018 to 4,144,315 pflD Position (kb) 4141 4142Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4140.667 kb on + strand, within frwCat 4140.667 kb on + strand, within frwCat 4140.685 kb on + strand, within frwCat 4140.685 kb on + strand, within frwCat 4140.685 kb on + strand, within frwCat 4140.693 kb on - strand, within frwCat 4140.756 kb on + strand, within frwCat 4140.805 kb on + strand, within frwCat 4140.808 kb on - strand, within frwCat 4140.827 kb on + strand, within frwCat 4140.827 kb on + strand, within frwCat 4140.830 kb on + strand, within frwCat 4140.895 kb on + strand, within frwCat 4140.895 kb on + strand, within frwCat 4140.951 kb on + strand, within frwCat 4141.002 kb on - strand, within frwCat 4141.010 kb on + strand, within frwCat 4141.010 kb on + strand, within frwCat 4141.051 kb on - strand, within frwCat 4141.066 kb on + strand, within frwCat 4141.069 kb on + strand, within frwCat 4141.069 kb on + strand, within frwCat 4141.069 kb on + strand, within frwCat 4141.092 kb on - strand, within frwCat 4141.105 kb on + strand, within frwCat 4141.130 kb on - strand, within frwCat 4141.133 kb on + strand, within frwCat 4141.154 kb on + strand, within frwCat 4141.167 kb on + strand, within frwCat 4141.191 kb on - strand, within frwCat 4141.194 kb on - strand, within frwCat 4141.194 kb on - strand, within frwCat 4141.218 kb on + strand, within frwCat 4141.218 kb on + strand, within frwCat 4141.255 kb on + strand, within frwCat 4141.255 kb on + strand, within frwCat 4141.311 kb on + strand, within frwCat 4141.311 kb on + strand, within frwCat 4141.320 kb on - strand, within frwCat 4141.320 kb on - strand, within frwCat 4141.320 kb on - strand, within frwCat 4141.441 kb on - strand, within frwCat 4141.448 kb on - strand, within frwCat 4141.448 kb on - strand, within frwCat 4141.449 kb on + strand, within frwCat 4141.450 kb on + strand, within frwCat 4141.494 kb on + strand, within frwCat 4141.549 kb on - strandat 4141.549 kb on - strandat 4141.676 kb on + strandat 4141.692 kb on - strand, within frwBat 4141.692 kb on - strand, within frwBat 4141.816 kb on + strand, within frwBat 4141.832 kb on + strand, within frwBat 4141.832 kb on + strand, within frwBat 4141.969 kb on + strandat 4142.009 kb on + strandat 4142.028 kb on + strandat 4142.092 kb on + strandat 4142.204 kb on - strandat 4142.223 kb on + strandat 4142.225 kb on + strandat 4142.282 kb on + strand, within pflDat 4142.282 kb on + strand, within pflDat 4142.357 kb on - strand, within pflDat 4142.357 kb on - strand, within pflDat 4142.370 kb on - strand, within pflDat 4142.370 kb on - strand, within pflDat 4142.410 kb on + strand, within pflDat 4142.461 kb on + strand, within pflDat 4142.461 kb on + strand, within pflDat 4142.476 kb on - strand, within pflDat 4142.476 kb on - strand, within pflDat 4142.497 kb on - strand, within pflDat 4142.497 kb on - strand, within pflDat 4142.499 kb on + strand, within pflDat 4142.528 kb on - strand, within pflDat 4142.602 kb on + strand, within pflDat 4142.672 kb on + strand, within pflDat 4142.680 kb on + strand, within pflDat 4142.683 kb on - strand, within pflDat 4142.683 kb on - strand, within pflDat 4142.688 kb on - strand, within pflDat 4142.690 kb on + strand, within pflDat 4142.690 kb on + strand, within pflDat 4142.698 kb on + strand, within pflDat 4142.705 kb on + strand, within pflDat 4142.736 kb on + strand, within pflDat 4142.753 kb on - strand, within pflDat 4142.765 kb on - strand, within pflDat 4142.781 kb on + strand, within pflDat 4142.834 kb on + strand, within pflDat 4142.834 kb on + strand, within pflDat 4142.837 kb on + strand, within pflDat 4142.862 kb on - strand, within pflDat 4142.862 kb on - strand, within pflDat 4142.865 kb on + strand, within pflDat 4142.870 kb on + strand, within pflDat 4142.873 kb on - strand, within pflDat 4142.905 kb on + strand, within pflDat 4142.905 kb on + strand, within pflDat 4142.905 kb on + strand, within pflDat 4142.934 kb on + strand, within pflDat 4142.942 kb on - strand, within pflDat 4142.944 kb on + strand, within pflDat 4142.944 kb on + strand, within pflD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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4,140,667 + frwC b3949 0.11 -2.9
4,140,667 + frwC b3949 0.11 -1.2
4,140,685 + frwC b3949 0.12 +0.3
4,140,685 + frwC b3949 0.12 +0.5
4,140,685 + frwC b3949 0.12 -0.9
4,140,693 - frwC b3949 0.13 -0.4
4,140,756 + frwC b3949 0.19 +0.7
4,140,805 + frwC b3949 0.23 +2.8
4,140,808 - frwC b3949 0.24 -0.9
4,140,827 + frwC b3949 0.25 -0.3
4,140,827 + frwC b3949 0.25 +1.2
4,140,830 + frwC b3949 0.26 -0.2
4,140,895 + frwC b3949 0.32 +1.4
4,140,895 + frwC b3949 0.32 +0.7
4,140,951 + frwC b3949 0.37 -1.4
4,141,002 - frwC b3949 0.42 -0.1
4,141,010 + frwC b3949 0.42 +0.1
4,141,010 + frwC b3949 0.42 -1.7
4,141,051 - frwC b3949 0.46 +0.6
4,141,066 + frwC b3949 0.47 -0.6
4,141,069 + frwC b3949 0.48 -0.9
4,141,069 + frwC b3949 0.48 -0.2
4,141,069 + frwC b3949 0.48 -0.6
4,141,092 - frwC b3949 0.50 -0.2
4,141,105 + frwC b3949 0.51 -0.3
4,141,130 - frwC b3949 0.53 +1.2
4,141,133 + frwC b3949 0.54 +0.8
4,141,154 + frwC b3949 0.56 -1.9
4,141,167 + frwC b3949 0.57 -1.2
4,141,191 - frwC b3949 0.59 -1.3
4,141,194 - frwC b3949 0.59 +0.6
4,141,194 - frwC b3949 0.59 -2.5
4,141,218 + frwC b3949 0.62 -0.8
4,141,218 + frwC b3949 0.62 -0.5
4,141,255 + frwC b3949 0.65 +0.7
4,141,255 + frwC b3949 0.65 -1.6
4,141,311 + frwC b3949 0.70 -2.7
4,141,311 + frwC b3949 0.70 -0.4
4,141,320 - frwC b3949 0.71 -0.5
4,141,320 - frwC b3949 0.71 +2.1
4,141,320 - frwC b3949 0.71 -0.6
4,141,441 - frwC b3949 0.82 -0.4
4,141,448 - frwC b3949 0.83 -1.9
4,141,448 - frwC b3949 0.83 +0.8
4,141,449 + frwC b3949 0.83 +0.7
4,141,450 + frwC b3949 0.83 -0.0
4,141,494 + frwC b3949 0.87 -0.6
4,141,549 - -2.7
4,141,549 - +0.7
4,141,676 + -0.1
4,141,692 - frwB b3950 0.14 -1.5
4,141,692 - frwB b3950 0.14 -1.3
4,141,816 + frwB b3950 0.53 -0.9
4,141,832 + frwB b3950 0.58 +1.0
4,141,832 + frwB b3950 0.58 +0.6
4,141,969 + +1.8
4,142,009 + +0.2
4,142,028 + +0.6
4,142,092 + -0.8
4,142,204 - -0.1
4,142,223 + -0.4
4,142,225 + +2.2
4,142,282 + pflD b3951 0.11 +1.3
4,142,282 + pflD b3951 0.11 +0.0
4,142,357 - pflD b3951 0.15 +0.6
4,142,357 - pflD b3951 0.15 -2.7
4,142,370 - pflD b3951 0.15 -0.4
4,142,370 - pflD b3951 0.15 +0.4
4,142,410 + pflD b3951 0.17 +0.6
4,142,461 + pflD b3951 0.19 +1.6
4,142,461 + pflD b3951 0.19 -1.6
4,142,476 - pflD b3951 0.20 +1.7
4,142,476 - pflD b3951 0.20 -1.9
4,142,497 - pflD b3951 0.21 -2.2
4,142,497 - pflD b3951 0.21 -2.3
4,142,499 + pflD b3951 0.21 +0.7
4,142,528 - pflD b3951 0.22 +0.5
4,142,602 + pflD b3951 0.25 -0.1
4,142,672 + pflD b3951 0.28 -1.3
4,142,680 + pflD b3951 0.29 +1.1
4,142,683 - pflD b3951 0.29 +0.9
4,142,683 - pflD b3951 0.29 -0.7
4,142,688 - pflD b3951 0.29 +0.5
4,142,690 + pflD b3951 0.29 -0.4
4,142,690 + pflD b3951 0.29 -0.9
4,142,698 + pflD b3951 0.30 +2.4
4,142,705 + pflD b3951 0.30 -1.2
4,142,736 + pflD b3951 0.31 +0.8
4,142,753 - pflD b3951 0.32 +0.7
4,142,765 - pflD b3951 0.33 +0.1
4,142,781 + pflD b3951 0.33 -0.8
4,142,834 + pflD b3951 0.36 -0.3
4,142,834 + pflD b3951 0.36 +0.6
4,142,837 + pflD b3951 0.36 +0.4
4,142,862 - pflD b3951 0.37 +1.8
4,142,862 - pflD b3951 0.37 -0.4
4,142,865 + pflD b3951 0.37 +0.6
4,142,870 + pflD b3951 0.37 -0.2
4,142,873 - pflD b3951 0.37 +1.2
4,142,905 + pflD b3951 0.39 +0.5
4,142,905 + pflD b3951 0.39 -0.2
4,142,905 + pflD b3951 0.39 -0.6
4,142,934 + pflD b3951 0.40 +1.3
4,142,942 - pflD b3951 0.40 +0.3
4,142,944 + pflD b3951 0.40 +0.8
4,142,944 + pflD b3951 0.40 +1.4

Or see this region's nucleotide sequence