Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trkD and rbsD are separated by 166 nucleotides rbsD and rbsA are separated by 7 nucleotides rbsA and rbsC are separated by 4 nucleotides rbsC and rbsB are separated by 24 nucleotides
b3747: trkD - potassium transporter (NCBI), at 3,929,339 to 3,931,207
trkD
b3748: rbsD - D-ribose pyranase [EC:5.4.99.62] (from data) , at 3,931,374 to 3,931,793
rbsD
b3749: rbsA - fused D-ribose transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 3,931,801 to 3,933,306
rbsA
b3750: rbsC - ribose ABC transporter permease protein (NCBI), at 3,933,311 to 3,934,276
rbsC
b3751: rbsB - D-ribose transporter subunit (NCBI), at 3,934,301 to 3,935,191
rbsB
Position (kb)
3931
3932
3933
3934 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3930.801 kb on + strand, within trkD at 3930.809 kb on - strand, within trkD at 3930.809 kb on - strand, within trkD at 3930.812 kb on + strand, within trkD at 3930.855 kb on - strand, within trkD at 3930.866 kb on - strand, within trkD at 3930.866 kb on - strand, within trkD at 3930.868 kb on + strand, within trkD at 3930.871 kb on + strand, within trkD at 3930.884 kb on + strand, within trkD at 3930.900 kb on - strand, within trkD at 3930.903 kb on + strand, within trkD at 3930.907 kb on + strand, within trkD at 3930.952 kb on + strand, within trkD at 3931.004 kb on + strand, within trkD at 3931.035 kb on + strand at 3931.039 kb on - strand at 3931.070 kb on - strand at 3931.070 kb on - strand at 3931.109 kb on + strand at 3931.109 kb on + strand at 3931.111 kb on + strand at 3931.111 kb on + strand at 3931.111 kb on + strand at 3931.134 kb on - strand at 3931.138 kb on + strand at 3931.194 kb on - strand at 3931.254 kb on + strand at 3931.264 kb on + strand at 3931.264 kb on + strand at 3931.310 kb on + strand at 3931.327 kb on - strand at 3931.327 kb on - strand at 3931.330 kb on + strand at 3931.342 kb on - strand at 3931.399 kb on - strand at 3931.435 kb on - strand, within rbsD at 3931.443 kb on - strand, within rbsD at 3931.443 kb on - strand, within rbsD at 3931.443 kb on - strand, within rbsD at 3931.449 kb on - strand, within rbsD at 3931.456 kb on - strand, within rbsD at 3931.461 kb on + strand, within rbsD at 3931.461 kb on + strand, within rbsD at 3931.506 kb on + strand, within rbsD at 3931.512 kb on + strand, within rbsD at 3931.512 kb on + strand, within rbsD at 3931.513 kb on + strand, within rbsD at 3931.519 kb on - strand, within rbsD at 3931.533 kb on + strand, within rbsD at 3931.533 kb on + strand, within rbsD at 3931.553 kb on - strand, within rbsD at 3931.560 kb on - strand, within rbsD at 3931.560 kb on - strand, within rbsD at 3931.587 kb on - strand, within rbsD at 3931.605 kb on + strand, within rbsD at 3931.605 kb on + strand, within rbsD at 3931.632 kb on + strand, within rbsD at 3931.641 kb on - strand, within rbsD at 3931.658 kb on + strand, within rbsD at 3931.676 kb on + strand, within rbsD at 3931.676 kb on + strand, within rbsD at 3931.684 kb on - strand, within rbsD at 3931.684 kb on - strand, within rbsD at 3931.684 kb on - strand, within rbsD at 3931.684 kb on - strand, within rbsD at 3931.684 kb on - strand, within rbsD at 3931.813 kb on - strand at 3931.821 kb on - strand at 3931.908 kb on - strand at 3931.908 kb on - strand at 3931.990 kb on - strand, within rbsA at 3931.990 kb on - strand, within rbsA at 3932.032 kb on - strand, within rbsA at 3932.041 kb on + strand, within rbsA at 3932.062 kb on - strand, within rbsA at 3932.062 kb on - strand, within rbsA at 3932.080 kb on - strand, within rbsA at 3932.080 kb on - strand, within rbsA at 3932.127 kb on + strand, within rbsA at 3932.127 kb on + strand, within rbsA at 3932.204 kb on + strand, within rbsA at 3932.226 kb on + strand, within rbsA at 3932.234 kb on - strand, within rbsA at 3932.302 kb on - strand, within rbsA at 3932.340 kb on + strand, within rbsA at 3932.372 kb on + strand, within rbsA at 3932.383 kb on + strand, within rbsA at 3932.383 kb on + strand, within rbsA at 3932.383 kb on + strand, within rbsA at 3932.391 kb on - strand, within rbsA at 3932.456 kb on + strand, within rbsA at 3932.466 kb on + strand, within rbsA at 3932.481 kb on - strand, within rbsA at 3932.490 kb on - strand, within rbsA at 3932.542 kb on + strand, within rbsA at 3932.542 kb on + strand, within rbsA at 3932.555 kb on - strand, within rbsA at 3932.555 kb on - strand, within rbsA at 3932.578 kb on + strand, within rbsA at 3932.578 kb on + strand, within rbsA at 3932.589 kb on + strand, within rbsA at 3932.599 kb on + strand, within rbsA at 3932.599 kb on + strand, within rbsA at 3932.599 kb on + strand, within rbsA at 3932.599 kb on + strand, within rbsA at 3932.604 kb on - strand, within rbsA at 3932.684 kb on + strand, within rbsA at 3932.689 kb on + strand, within rbsA at 3932.689 kb on + strand, within rbsA at 3932.706 kb on + strand, within rbsA at 3932.706 kb on + strand, within rbsA at 3932.718 kb on + strand, within rbsA at 3932.720 kb on + strand, within rbsA at 3932.728 kb on + strand, within rbsA at 3932.728 kb on + strand, within rbsA at 3932.729 kb on + strand, within rbsA at 3932.731 kb on - strand, within rbsA at 3932.738 kb on + strand, within rbsA at 3932.738 kb on + strand, within rbsA at 3932.754 kb on - strand, within rbsA at 3932.754 kb on - strand, within rbsA at 3932.796 kb on - strand, within rbsA at 3932.827 kb on - strand, within rbsA at 3932.874 kb on + strand, within rbsA at 3932.874 kb on + strand, within rbsA at 3932.876 kb on - strand, within rbsA at 3932.876 kb on - strand, within rbsA at 3932.902 kb on - strand, within rbsA at 3932.927 kb on - strand, within rbsA at 3932.927 kb on - strand, within rbsA at 3932.968 kb on + strand, within rbsA at 3933.015 kb on + strand, within rbsA at 3933.015 kb on + strand, within rbsA at 3933.027 kb on - strand, within rbsA at 3933.064 kb on - strand, within rbsA at 3933.070 kb on - strand, within rbsA at 3933.070 kb on - strand, within rbsA at 3933.070 kb on - strand, within rbsA at 3933.082 kb on - strand, within rbsA at 3933.118 kb on - strand, within rbsA at 3933.118 kb on - strand, within rbsA at 3933.145 kb on + strand, within rbsA at 3933.164 kb on + strand at 3933.185 kb on - strand at 3933.200 kb on - strand at 3933.247 kb on + strand at 3933.256 kb on - strand at 3933.256 kb on - strand at 3933.258 kb on + strand at 3933.258 kb on + strand at 3933.262 kb on + strand at 3933.269 kb on + strand at 3933.282 kb on + strand at 3933.317 kb on + strand at 3933.317 kb on + strand at 3933.324 kb on - strand at 3933.345 kb on - strand at 3933.345 kb on - strand at 3933.384 kb on + strand at 3933.460 kb on + strand, within rbsC at 3933.481 kb on - strand, within rbsC at 3933.495 kb on + strand, within rbsC at 3933.495 kb on + strand, within rbsC at 3933.540 kb on + strand, within rbsC at 3933.548 kb on - strand, within rbsC at 3933.548 kb on - strand, within rbsC at 3933.592 kb on + strand, within rbsC at 3933.592 kb on + strand, within rbsC at 3933.755 kb on + strand, within rbsC at 3933.755 kb on + strand, within rbsC at 3933.768 kb on + strand, within rbsC at 3933.815 kb on - strand, within rbsC at 3933.815 kb on - strand, within rbsC at 3933.848 kb on - strand, within rbsC at 3933.856 kb on + strand, within rbsC at 3933.866 kb on + strand, within rbsC at 3933.876 kb on - strand, within rbsC at 3933.878 kb on - strand, within rbsC at 3933.883 kb on - strand, within rbsC at 3933.883 kb on - strand, within rbsC at 3933.886 kb on - strand, within rbsC at 3933.890 kb on + strand, within rbsC at 3933.898 kb on + strand, within rbsC at 3933.898 kb on + strand, within rbsC at 3933.898 kb on - strand, within rbsC at 3933.924 kb on - strand, within rbsC at 3933.924 kb on - strand, within rbsC at 3933.937 kb on + strand, within rbsC at 3933.950 kb on + strand, within rbsC at 3933.950 kb on + strand, within rbsC at 3934.001 kb on - strand, within rbsC at 3934.001 kb on - strand, within rbsC at 3934.003 kb on - strand, within rbsC at 3934.051 kb on - strand, within rbsC at 3934.056 kb on - strand, within rbsC at 3934.075 kb on + strand at 3934.094 kb on + strand, within rbsC at 3934.097 kb on - strand, within rbsC at 3934.101 kb on - strand, within rbsC at 3934.110 kb on - strand, within rbsC at 3934.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,930,801 + trkD b3747 0.78 +0.8 3,930,809 - trkD b3747 0.79 -0.1 3,930,809 - trkD b3747 0.79 -0.1 3,930,812 + trkD b3747 0.79 +0.8 3,930,855 - trkD b3747 0.81 -1.1 3,930,866 - trkD b3747 0.82 +0.5 3,930,866 - trkD b3747 0.82 -0.1 3,930,868 + trkD b3747 0.82 -0.5 3,930,871 + trkD b3747 0.82 +0.2 3,930,884 + trkD b3747 0.83 +0.5 3,930,900 - trkD b3747 0.84 -1.9 3,930,903 + trkD b3747 0.84 -0.8 3,930,907 + trkD b3747 0.84 -0.9 3,930,952 + trkD b3747 0.86 -0.5 3,931,004 + trkD b3747 0.89 -0.7 3,931,035 + +0.7 3,931,039 - +0.5 3,931,070 - +0.5 3,931,070 - -0.1 3,931,109 + -1.5 3,931,109 + -0.2 3,931,111 + -0.5 3,931,111 + +1.5 3,931,111 + -1.7 3,931,134 - -0.3 3,931,138 + -0.2 3,931,194 - -0.3 3,931,254 + +0.1 3,931,264 + +1.2 3,931,264 + +1.7 3,931,310 + -0.9 3,931,327 - -0.7 3,931,327 - +0.6 3,931,330 + +0.7 3,931,342 - +0.2 3,931,399 - -0.9 3,931,435 - rbsD b3748 0.15 -0.3 3,931,443 - rbsD b3748 0.16 -3.0 3,931,443 - rbsD b3748 0.16 +0.3 3,931,443 - rbsD b3748 0.16 -1.7 3,931,449 - rbsD b3748 0.18 -0.6 3,931,456 - rbsD b3748 0.20 -0.3 3,931,461 + rbsD b3748 0.21 +1.5 3,931,461 + rbsD b3748 0.21 -2.2 3,931,506 + rbsD b3748 0.31 -0.8 3,931,512 + rbsD b3748 0.33 +0.6 3,931,512 + rbsD b3748 0.33 +1.1 3,931,513 + rbsD b3748 0.33 -0.3 3,931,519 - rbsD b3748 0.35 -0.3 3,931,533 + rbsD b3748 0.38 -1.5 3,931,533 + rbsD b3748 0.38 +0.1 3,931,553 - rbsD b3748 0.43 -0.0 3,931,560 - rbsD b3748 0.44 +0.2 3,931,560 - rbsD b3748 0.44 +1.1 3,931,587 - rbsD b3748 0.51 +0.3 3,931,605 + rbsD b3748 0.55 +0.3 3,931,605 + rbsD b3748 0.55 -1.3 3,931,632 + rbsD b3748 0.61 -1.0 3,931,641 - rbsD b3748 0.64 +1.4 3,931,658 + rbsD b3748 0.68 +0.2 3,931,676 + rbsD b3748 0.72 -0.3 3,931,676 + rbsD b3748 0.72 -2.5 3,931,684 - rbsD b3748 0.74 +1.0 3,931,684 - rbsD b3748 0.74 -1.6 3,931,684 - rbsD b3748 0.74 +0.5 3,931,684 - rbsD b3748 0.74 +0.6 3,931,684 - rbsD b3748 0.74 -1.2 3,931,813 - +1.8 3,931,821 - +0.7 3,931,908 - -1.3 3,931,908 - -1.7 3,931,990 - rbsA b3749 0.13 +0.8 3,931,990 - rbsA b3749 0.13 -1.5 3,932,032 - rbsA b3749 0.15 +0.2 3,932,041 + rbsA b3749 0.16 -1.1 3,932,062 - rbsA b3749 0.17 -0.7 3,932,062 - rbsA b3749 0.17 +0.2 3,932,080 - rbsA b3749 0.19 -0.9 3,932,080 - rbsA b3749 0.19 -2.3 3,932,127 + rbsA b3749 0.22 +2.0 3,932,127 + rbsA b3749 0.22 +0.1 3,932,204 + rbsA b3749 0.27 -0.1 3,932,226 + rbsA b3749 0.28 +2.8 3,932,234 - rbsA b3749 0.29 -1.9 3,932,302 - rbsA b3749 0.33 +0.5 3,932,340 + rbsA b3749 0.36 +3.3 3,932,372 + rbsA b3749 0.38 +1.9 3,932,383 + rbsA b3749 0.39 +1.5 3,932,383 + rbsA b3749 0.39 +0.4 3,932,383 + rbsA b3749 0.39 -0.5 3,932,391 - rbsA b3749 0.39 +0.2 3,932,456 + rbsA b3749 0.43 +3.9 3,932,466 + rbsA b3749 0.44 +2.5 3,932,481 - rbsA b3749 0.45 -0.5 3,932,490 - rbsA b3749 0.46 -0.7 3,932,542 + rbsA b3749 0.49 +1.2 3,932,542 + rbsA b3749 0.49 +1.7 3,932,555 - rbsA b3749 0.50 +0.9 3,932,555 - rbsA b3749 0.50 -1.6 3,932,578 + rbsA b3749 0.52 -0.1 3,932,578 + rbsA b3749 0.52 +0.5 3,932,589 + rbsA b3749 0.52 +3.6 3,932,599 + rbsA b3749 0.53 -0.5 3,932,599 + rbsA b3749 0.53 +2.5 3,932,599 + rbsA b3749 0.53 +1.6 3,932,599 + rbsA b3749 0.53 +1.3 3,932,604 - rbsA b3749 0.53 -1.2 3,932,684 + rbsA b3749 0.59 +1.3 3,932,689 + rbsA b3749 0.59 -2.1 3,932,689 + rbsA b3749 0.59 +1.7 3,932,706 + rbsA b3749 0.60 +2.3 3,932,706 + rbsA b3749 0.60 +0.8 3,932,718 + rbsA b3749 0.61 +3.5 3,932,720 + rbsA b3749 0.61 +3.1 3,932,728 + rbsA b3749 0.62 +2.0 3,932,728 + rbsA b3749 0.62 -0.8 3,932,729 + rbsA b3749 0.62 -0.3 3,932,731 - rbsA b3749 0.62 +0.6 3,932,738 + rbsA b3749 0.62 +2.4 3,932,738 + rbsA b3749 0.62 +2.6 3,932,754 - rbsA b3749 0.63 +0.5 3,932,754 - rbsA b3749 0.63 -0.6 3,932,796 - rbsA b3749 0.66 +1.1 3,932,827 - rbsA b3749 0.68 -1.0 3,932,874 + rbsA b3749 0.71 +1.7 3,932,874 + rbsA b3749 0.71 +2.0 3,932,876 - rbsA b3749 0.71 +1.9 3,932,876 - rbsA b3749 0.71 -1.7 3,932,902 - rbsA b3749 0.73 +1.0 3,932,927 - rbsA b3749 0.75 +0.7 3,932,927 - rbsA b3749 0.75 -0.3 3,932,968 + rbsA b3749 0.77 +2.6 3,933,015 + rbsA b3749 0.81 +2.8 3,933,015 + rbsA b3749 0.81 +2.8 3,933,027 - rbsA b3749 0.81 -0.2 3,933,064 - rbsA b3749 0.84 -0.2 3,933,070 - rbsA b3749 0.84 -1.3 3,933,070 - rbsA b3749 0.84 +0.3 3,933,070 - rbsA b3749 0.84 -0.7 3,933,082 - rbsA b3749 0.85 +1.0 3,933,118 - rbsA b3749 0.87 -0.9 3,933,118 - rbsA b3749 0.87 -2.0 3,933,145 + rbsA b3749 0.89 +2.9 3,933,164 + +0.5 3,933,185 - -0.3 3,933,200 - +0.6 3,933,247 + +2.4 3,933,256 - -0.2 3,933,256 - +0.3 3,933,258 + -1.1 3,933,258 + -2.4 3,933,262 + +2.8 3,933,269 + -0.0 3,933,282 + -1.1 3,933,317 + -0.2 3,933,317 + +0.5 3,933,324 - +0.8 3,933,345 - -0.3 3,933,345 - +0.6 3,933,384 + -1.0 3,933,460 + rbsC b3750 0.15 +1.3 3,933,481 - rbsC b3750 0.18 +0.8 3,933,495 + rbsC b3750 0.19 +2.0 3,933,495 + rbsC b3750 0.19 +2.1 3,933,540 + rbsC b3750 0.24 +2.3 3,933,548 - rbsC b3750 0.25 -2.5 3,933,548 - rbsC b3750 0.25 -1.5 3,933,592 + rbsC b3750 0.29 +3.4 3,933,592 + rbsC b3750 0.29 +2.6 3,933,755 + rbsC b3750 0.46 -0.6 3,933,755 + rbsC b3750 0.46 -0.1 3,933,768 + rbsC b3750 0.47 +2.6 3,933,815 - rbsC b3750 0.52 +0.2 3,933,815 - rbsC b3750 0.52 +0.8 3,933,848 - rbsC b3750 0.56 -0.5 3,933,856 + rbsC b3750 0.56 +2.5 3,933,866 + rbsC b3750 0.57 +0.9 3,933,876 - rbsC b3750 0.58 -1.7 3,933,878 - rbsC b3750 0.59 -0.9 3,933,883 - rbsC b3750 0.59 -1.0 3,933,883 - rbsC b3750 0.59 -2.5 3,933,886 - rbsC b3750 0.60 +0.6 3,933,890 + rbsC b3750 0.60 +2.8 3,933,898 + rbsC b3750 0.61 +2.1 3,933,898 + rbsC b3750 0.61 +2.3 3,933,898 - rbsC b3750 0.61 -1.7 3,933,924 - rbsC b3750 0.63 +1.3 3,933,924 - rbsC b3750 0.63 +0.9 3,933,937 + rbsC b3750 0.65 +1.9 3,933,950 + rbsC b3750 0.66 +2.3 3,933,950 + rbsC b3750 0.66 +2.4 3,934,001 - rbsC b3750 0.71 +1.5 3,934,001 - rbsC b3750 0.71 -0.4 3,934,003 - rbsC b3750 0.72 -1.3 3,934,051 - rbsC b3750 0.77 +0.1 3,934,056 - rbsC b3750 0.77 +0.1 3,934,075 + +2.0 3,934,094 + rbsC b3750 0.81 +3.5 3,934,097 - rbsC b3750 0.81 -0.0 3,934,101 - rbsC b3750 0.82 -0.1 3,934,110 - rbsC b3750 0.83 -0.6 3,934,269 - -0.5
Or see this region's nucleotide sequence