Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nikD and nikE overlap by 4 nucleotides nikE and nikR are separated by 5 nucleotides nikR and rhsB are separated by 202 nucleotides
b3479: nikD - nickel transporter subunit (NCBI), at 3,615,038 to 3,615,802
nikD
b3480: nikE - nickel transporter subunit (NCBI), at 3,615,799 to 3,616,605
nikE
b3481: nikR - nickel responsive regulator (NCBI), at 3,616,611 to 3,617,012
nikR
b3482: rhsB - rhsB element core protein RshB (NCBI), at 3,617,215 to 3,621,450
rhsB
Position (kb)
3616
3617
3618 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3615.706 kb on - strand, within nikD at 3615.708 kb on + strand, within nikD at 3615.708 kb on + strand, within nikD at 3615.711 kb on - strand, within nikD at 3615.729 kb on + strand at 3615.735 kb on + strand at 3615.735 kb on + strand at 3615.783 kb on + strand at 3615.783 kb on + strand at 3615.820 kb on + strand at 3615.892 kb on + strand, within nikE at 3615.892 kb on + strand, within nikE at 3615.899 kb on + strand, within nikE at 3615.966 kb on + strand, within nikE at 3615.966 kb on + strand, within nikE at 3615.966 kb on + strand, within nikE at 3615.977 kb on + strand, within nikE at 3616.047 kb on + strand, within nikE at 3616.078 kb on + strand, within nikE at 3616.091 kb on + strand, within nikE at 3616.110 kb on + strand, within nikE at 3616.157 kb on - strand, within nikE at 3616.224 kb on + strand, within nikE at 3616.287 kb on - strand, within nikE at 3616.424 kb on - strand, within nikE at 3616.444 kb on - strand, within nikE at 3616.444 kb on - strand, within nikE at 3616.490 kb on + strand, within nikE at 3616.572 kb on - strand at 3616.691 kb on - strand, within nikR at 3616.721 kb on - strand, within nikR at 3616.723 kb on + strand, within nikR at 3616.743 kb on + strand, within nikR at 3616.764 kb on - strand, within nikR at 3616.793 kb on - strand, within nikR at 3616.793 kb on - strand, within nikR at 3616.815 kb on - strand, within nikR at 3616.853 kb on + strand, within nikR at 3616.853 kb on + strand, within nikR at 3616.872 kb on + strand, within nikR at 3616.932 kb on + strand, within nikR at 3616.937 kb on + strand, within nikR at 3617.048 kb on - strand at 3617.063 kb on + strand at 3617.063 kb on + strand at 3617.065 kb on - strand at 3617.069 kb on - strand at 3617.069 kb on - strand at 3617.069 kb on - strand at 3617.083 kb on - strand at 3617.083 kb on - strand at 3617.085 kb on + strand at 3617.177 kb on + strand at 3617.200 kb on + strand at 3617.251 kb on + strand at 3617.251 kb on + strand at 3617.290 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,615,706 - nikD b3479 0.87 -1.5 3,615,708 + nikD b3479 0.88 -2.4 3,615,708 + nikD b3479 0.88 -0.8 3,615,711 - nikD b3479 0.88 -2.5 3,615,729 + -3.1 3,615,735 + -2.3 3,615,735 + -1.7 3,615,783 + -2.0 3,615,783 + -2.5 3,615,820 + +0.1 3,615,892 + nikE b3480 0.12 -1.6 3,615,892 + nikE b3480 0.12 -0.1 3,615,899 + nikE b3480 0.12 -1.7 3,615,966 + nikE b3480 0.21 -2.6 3,615,966 + nikE b3480 0.21 -0.4 3,615,966 + nikE b3480 0.21 -0.9 3,615,977 + nikE b3480 0.22 -1.3 3,616,047 + nikE b3480 0.31 -1.6 3,616,078 + nikE b3480 0.35 -2.3 3,616,091 + nikE b3480 0.36 -0.6 3,616,110 + nikE b3480 0.39 -1.4 3,616,157 - nikE b3480 0.44 +2.4 3,616,224 + nikE b3480 0.53 -3.8 3,616,287 - nikE b3480 0.60 -0.4 3,616,424 - nikE b3480 0.77 -0.2 3,616,444 - nikE b3480 0.80 -0.6 3,616,444 - nikE b3480 0.80 -0.8 3,616,490 + nikE b3480 0.86 -0.9 3,616,572 - -0.9 3,616,691 - nikR b3481 0.20 -1.2 3,616,721 - nikR b3481 0.27 +1.2 3,616,723 + nikR b3481 0.28 +0.8 3,616,743 + nikR b3481 0.33 -1.6 3,616,764 - nikR b3481 0.38 +0.8 3,616,793 - nikR b3481 0.45 -0.6 3,616,793 - nikR b3481 0.45 -1.0 3,616,815 - nikR b3481 0.51 -2.0 3,616,853 + nikR b3481 0.60 +0.7 3,616,853 + nikR b3481 0.60 -2.2 3,616,872 + nikR b3481 0.65 +0.5 3,616,932 + nikR b3481 0.80 -1.5 3,616,937 + nikR b3481 0.81 -0.2 3,617,048 - -1.6 3,617,063 + -2.8 3,617,063 + +0.7 3,617,065 - +0.3 3,617,069 - -0.8 3,617,069 - +0.7 3,617,069 - -0.2 3,617,083 - +0.7 3,617,083 - -1.6 3,617,085 + -0.5 3,617,177 + +0.6 3,617,200 + +1.5 3,617,251 + +0.0 3,617,251 + -1.4 3,617,290 + -2.3
Or see this region's nucleotide sequence