Strain Fitness in Escherichia coli BW25113 around b3222

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhcG and yhcH are separated by 59 nucleotidesyhcH and yhcI overlap by 4 nucleotidesyhcI and nanE overlap by 4 nucleotidesnanE and nanT are separated by 47 nucleotides b3220: yhcG - hypothetical protein (NCBI), at 3,365,849 to 3,366,976 yhcG b3221: yhcH - hypothetical protein (NCBI), at 3,367,036 to 3,367,500 yhcH b3222: yhcI - putative NAGC-like transcriptional regulator (VIMSS), at 3,367,497 to 3,368,372 yhcI b3223: nanE - predicted N-acetylmannosamine-6-P epimerase (NCBI), at 3,368,369 to 3,369,058 nanE b3224: nanT - sialic acid transporter (VIMSS), at 3,369,106 to 3,370,596 nanT Position (kb) 3367 3368 3369Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3366.683 kb on - strand, within yhcGat 3366.683 kb on - strand, within yhcGat 3366.684 kb on + strand, within yhcGat 3366.684 kb on + strand, within yhcGat 3366.692 kb on - strand, within yhcGat 3366.720 kb on + strand, within yhcGat 3366.760 kb on + strand, within yhcGat 3366.760 kb on + strand, within yhcGat 3366.808 kb on + strand, within yhcGat 3366.856 kb on - strand, within yhcGat 3366.857 kb on + strand, within yhcGat 3366.906 kb on + strandat 3366.924 kb on - strandat 3366.955 kb on - strandat 3366.960 kb on + strandat 3366.960 kb on + strandat 3366.960 kb on + strandat 3366.967 kb on - strandat 3366.968 kb on - strandat 3367.219 kb on - strand, within yhcHat 3367.221 kb on + strand, within yhcHat 3367.238 kb on + strand, within yhcHat 3367.246 kb on - strand, within yhcHat 3367.260 kb on - strand, within yhcHat 3367.273 kb on - strand, within yhcHat 3367.273 kb on - strand, within yhcHat 3367.348 kb on - strand, within yhcHat 3367.386 kb on - strand, within yhcHat 3367.386 kb on - strand, within yhcHat 3367.444 kb on - strand, within yhcHat 3367.452 kb on - strand, within yhcHat 3367.454 kb on + strandat 3367.480 kb on - strandat 3367.480 kb on - strandat 3367.489 kb on + strandat 3367.523 kb on - strandat 3367.600 kb on + strand, within yhcIat 3367.600 kb on + strand, within yhcIat 3367.666 kb on - strand, within yhcIat 3367.678 kb on + strand, within yhcIat 3367.678 kb on + strand, within yhcIat 3367.691 kb on + strand, within yhcIat 3367.691 kb on + strand, within yhcIat 3367.725 kb on + strand, within yhcIat 3367.728 kb on + strand, within yhcIat 3367.768 kb on - strand, within yhcIat 3367.855 kb on - strand, within yhcIat 3367.855 kb on - strand, within yhcIat 3367.901 kb on + strand, within yhcIat 3368.002 kb on + strand, within yhcIat 3368.179 kb on - strand, within yhcIat 3368.179 kb on - strand, within yhcIat 3368.184 kb on - strand, within yhcIat 3368.285 kb on + strandat 3368.327 kb on - strandat 3368.368 kb on + strandat 3368.585 kb on + strand, within nanEat 3368.593 kb on - strand, within nanEat 3368.696 kb on - strand, within nanEat 3368.699 kb on + strand, within nanEat 3368.699 kb on + strand, within nanEat 3368.703 kb on + strand, within nanEat 3368.768 kb on + strand, within nanEat 3368.780 kb on + strand, within nanEat 3368.791 kb on + strand, within nanEat 3368.791 kb on + strand, within nanEat 3368.887 kb on + strand, within nanEat 3368.989 kb on - strand, within nanEat 3368.989 kb on - strand, within nanEat 3369.084 kb on + strandat 3369.297 kb on + strand, within nanT

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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3,366,683 - yhcG b3220 0.74 +0.3
3,366,683 - yhcG b3220 0.74 -0.3
3,366,684 + yhcG b3220 0.74 -1.0
3,366,684 + yhcG b3220 0.74 +0.0
3,366,692 - yhcG b3220 0.75 +1.0
3,366,720 + yhcG b3220 0.77 -0.9
3,366,760 + yhcG b3220 0.81 +0.3
3,366,760 + yhcG b3220 0.81 -1.4
3,366,808 + yhcG b3220 0.85 -0.0
3,366,856 - yhcG b3220 0.89 -0.9
3,366,857 + yhcG b3220 0.89 -0.7
3,366,906 + -1.7
3,366,924 - +0.8
3,366,955 - -1.5
3,366,960 + -2.3
3,366,960 + -2.4
3,366,960 + +0.1
3,366,967 - -2.5
3,366,968 - +0.3
3,367,219 - yhcH b3221 0.39 -1.5
3,367,221 + yhcH b3221 0.40 -0.4
3,367,238 + yhcH b3221 0.43 -0.0
3,367,246 - yhcH b3221 0.45 +1.0
3,367,260 - yhcH b3221 0.48 -2.3
3,367,273 - yhcH b3221 0.51 +0.2
3,367,273 - yhcH b3221 0.51 -0.7
3,367,348 - yhcH b3221 0.67 -0.0
3,367,386 - yhcH b3221 0.75 -2.8
3,367,386 - yhcH b3221 0.75 +0.4
3,367,444 - yhcH b3221 0.88 +1.4
3,367,452 - yhcH b3221 0.89 -0.0
3,367,454 + +0.3
3,367,480 - -1.6
3,367,480 - -0.2
3,367,489 + -0.6
3,367,523 - +0.7
3,367,600 + yhcI b3222 0.12 -0.9
3,367,600 + yhcI b3222 0.12 +1.1
3,367,666 - yhcI b3222 0.19 -1.6
3,367,678 + yhcI b3222 0.21 +1.4
3,367,678 + yhcI b3222 0.21 +1.4
3,367,691 + yhcI b3222 0.22 -0.2
3,367,691 + yhcI b3222 0.22 +0.3
3,367,725 + yhcI b3222 0.26 +2.6
3,367,728 + yhcI b3222 0.26 -1.7
3,367,768 - yhcI b3222 0.31 -1.0
3,367,855 - yhcI b3222 0.41 -0.3
3,367,855 - yhcI b3222 0.41 -0.5
3,367,901 + yhcI b3222 0.46 -1.8
3,368,002 + yhcI b3222 0.58 -1.9
3,368,179 - yhcI b3222 0.78 -0.1
3,368,179 - yhcI b3222 0.78 -1.9
3,368,184 - yhcI b3222 0.78 -0.1
3,368,285 + +0.9
3,368,327 - +0.2
3,368,368 + -0.9
3,368,585 + nanE b3223 0.31 -0.8
3,368,593 - nanE b3223 0.32 -0.5
3,368,696 - nanE b3223 0.47 +0.2
3,368,699 + nanE b3223 0.48 -1.2
3,368,699 + nanE b3223 0.48 +0.4
3,368,703 + nanE b3223 0.48 -2.5
3,368,768 + nanE b3223 0.58 -0.0
3,368,780 + nanE b3223 0.60 +0.4
3,368,791 + nanE b3223 0.61 +0.2
3,368,791 + nanE b3223 0.61 +0.6
3,368,887 + nanE b3223 0.75 +0.7
3,368,989 - nanE b3223 0.90 -1.0
3,368,989 - nanE b3223 0.90 +1.8
3,369,084 + -0.7
3,369,297 + nanT b3224 0.13 +0.2

Or see this region's nucleotide sequence