Strain Fitness in Escherichia coli BW25113 around b3166

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpnp and sraG are separated by 56 nucleotidessraG and rpsO are separated by 16 nucleotidesrpsO and truB are separated by 148 nucleotidestruB and rbfA overlap by 1 nucleotidesrbfA and infB are separated by 163 nucleotides b3164: pnp - polynucleotide phosphorylase/polyadenylase (RefSeq), at 3,307,055 to 3,309,190 pnp b4449: sraG - unknown RNA (NCBI), at 3,309,247 to 3,309,420 sraG b3165: rpsO - 30S ribosomal protein S15 (NCBI), at 3,309,437 to 3,309,706 rpsO b3166: truB - tRNA pseudouridine synthase B (NCBI), at 3,309,855 to 3,310,799 truB b3167: rbfA - ribosome-binding factor A (NCBI), at 3,310,799 to 3,311,200 rbfA b3168: infB - translation initiation factor IF-2 (NCBI), at 3,311,364 to 3,314,036 infB Position (kb) 3309 3310 3311Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3309.216 kb on - strandat 3309.239 kb on - strandat 3309.437 kb on - strandat 3309.823 kb on - strandat 3309.823 kb on - strandat 3309.838 kb on - strandat 3309.987 kb on - strand, within truBat 3309.990 kb on - strand, within truBat 3310.065 kb on + strand, within truBat 3310.085 kb on - strand, within truBat 3310.126 kb on + strand, within truBat 3310.148 kb on + strand, within truBat 3310.148 kb on + strand, within truBat 3310.202 kb on + strand, within truBat 3310.210 kb on - strand, within truBat 3310.242 kb on + strand, within truBat 3310.371 kb on - strand, within truBat 3310.487 kb on + strand, within truBat 3310.523 kb on - strand, within truBat 3310.539 kb on + strand, within truBat 3310.545 kb on - strand, within truBat 3310.562 kb on + strand, within truBat 3310.562 kb on + strand, within truBat 3310.587 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.655 kb on + strand, within truBat 3310.682 kb on + strand, within truBat 3310.695 kb on - strand, within truBat 3310.700 kb on - strand, within truBat 3310.700 kb on - strand, within truBat 3310.723 kb on + strandat 3310.731 kb on - strandat 3310.736 kb on - strandat 3310.800 kb on - strandat 3310.800 kb on - strandat 3310.805 kb on - strandat 3310.845 kb on + strand, within rbfAat 3310.909 kb on - strand, within rbfAat 3311.290 kb on - strandat 3311.290 kb on - strandat 3311.382 kb on - strandat 3311.409 kb on - strandat 3311.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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3,309,216 - +1.9
3,309,239 - +0.0
3,309,437 - -0.6
3,309,823 - -1.5
3,309,823 - -0.1
3,309,838 - -2.5
3,309,987 - truB b3166 0.14 -2.7
3,309,990 - truB b3166 0.14 -2.4
3,310,065 + truB b3166 0.22 -1.1
3,310,085 - truB b3166 0.24 -2.5
3,310,126 + truB b3166 0.29 -2.6
3,310,148 + truB b3166 0.31 -2.5
3,310,148 + truB b3166 0.31 +0.3
3,310,202 + truB b3166 0.37 -2.9
3,310,210 - truB b3166 0.38 -3.0
3,310,242 + truB b3166 0.41 -2.0
3,310,371 - truB b3166 0.55 +0.3
3,310,487 + truB b3166 0.67 -3.4
3,310,523 - truB b3166 0.71 -1.5
3,310,539 + truB b3166 0.72 +0.1
3,310,545 - truB b3166 0.73 -0.5
3,310,562 + truB b3166 0.75 -2.1
3,310,562 + truB b3166 0.75 +0.3
3,310,587 + truB b3166 0.77 -1.1
3,310,655 + truB b3166 0.85 -1.4
3,310,655 + truB b3166 0.85 -2.2
3,310,682 + truB b3166 0.88 -4.1
3,310,695 - truB b3166 0.89 -0.3
3,310,700 - truB b3166 0.89 -1.0
3,310,700 - truB b3166 0.89 -2.6
3,310,723 + -1.9
3,310,731 - -2.6
3,310,736 - -2.2
3,310,800 - -0.9
3,310,800 - +0.0
3,310,805 - -1.6
3,310,845 + rbfA b3167 0.11 -1.3
3,310,909 - rbfA b3167 0.27 -0.7
3,311,290 - -0.7
3,311,290 - +1.9
3,311,382 - +0.7
3,311,409 - +1.3
3,311,409 - +1.3

Or see this region's nucleotide sequence