Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhbS and yhbT overlap by 7 nucleotides yhbT and yhbU are separated by 208 nucleotides yhbU and yhbV are separated by 8 nucleotides yhbV and yhbW are separated by 80 nucleotides
b3156: yhbS - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 3,298,277 to 3,298,780
yhbS
b3157: yhbT - predicted lipid carrier protein (NCBI), at 3,298,774 to 3,299,298
yhbT
b3158: yhbU - predicted peptidase (collagenase-like) (NCBI), at 3,299,507 to 3,300,502
yhbU
b3159: yhbV - orf, hypothetical protein (VIMSS), at 3,300,511 to 3,301,389
yhbV
b3160: yhbW - predicted enzyme (NCBI), at 3,301,470 to 3,302,477
yhbW
Position (kb)
3299
3300
3301 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3298.578 kb on + strand, within yhbS at 3298.578 kb on + strand, within yhbS at 3298.591 kb on - strand, within yhbS at 3298.635 kb on - strand, within yhbS at 3298.635 kb on - strand, within yhbS at 3298.649 kb on - strand, within yhbS at 3298.724 kb on + strand, within yhbS at 3298.737 kb on + strand at 3298.737 kb on + strand at 3298.858 kb on + strand, within yhbT at 3298.864 kb on + strand, within yhbT at 3298.951 kb on - strand, within yhbT at 3299.033 kb on - strand, within yhbT at 3299.033 kb on - strand, within yhbT at 3299.115 kb on + strand, within yhbT at 3299.123 kb on - strand, within yhbT at 3299.123 kb on - strand, within yhbT at 3299.220 kb on - strand, within yhbT at 3299.399 kb on - strand at 3299.399 kb on - strand at 3299.515 kb on + strand at 3299.515 kb on + strand at 3299.550 kb on - strand at 3299.626 kb on + strand, within yhbU at 3299.785 kb on - strand, within yhbU at 3299.785 kb on - strand, within yhbU at 3299.809 kb on - strand, within yhbU at 3299.857 kb on + strand, within yhbU at 3299.860 kb on - strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.968 kb on + strand, within yhbU at 3299.981 kb on - strand, within yhbU at 3300.162 kb on + strand, within yhbU at 3300.216 kb on - strand, within yhbU at 3300.216 kb on - strand, within yhbU at 3300.225 kb on - strand, within yhbU at 3300.240 kb on - strand, within yhbU at 3300.349 kb on - strand, within yhbU at 3300.349 kb on - strand, within yhbU at 3300.388 kb on + strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.396 kb on - strand, within yhbU at 3300.451 kb on + strand at 3300.451 kb on + strand at 3300.451 kb on + strand at 3300.451 kb on + strand at 3300.476 kb on + strand at 3300.532 kb on + strand at 3300.540 kb on - strand at 3300.541 kb on - strand at 3300.543 kb on - strand at 3300.543 kb on - strand at 3300.545 kb on + strand at 3300.630 kb on - strand, within yhbV at 3300.632 kb on - strand, within yhbV at 3300.729 kb on + strand, within yhbV at 3300.742 kb on - strand, within yhbV at 3300.742 kb on - strand, within yhbV at 3300.742 kb on - strand, within yhbV at 3300.761 kb on + strand, within yhbV at 3300.861 kb on + strand, within yhbV at 3300.917 kb on - strand, within yhbV at 3300.922 kb on - strand, within yhbV at 3300.957 kb on + strand, within yhbV at 3300.993 kb on + strand, within yhbV at 3300.993 kb on + strand, within yhbV at 3301.040 kb on - strand, within yhbV at 3301.094 kb on - strand, within yhbV at 3301.094 kb on - strand, within yhbV at 3301.100 kb on + strand, within yhbV at 3301.100 kb on + strand, within yhbV at 3301.230 kb on + strand, within yhbV at 3301.230 kb on + strand, within yhbV at 3301.245 kb on + strand, within yhbV at 3301.245 kb on + strand, within yhbV at 3301.286 kb on - strand, within yhbV at 3301.286 kb on - strand, within yhbV at 3301.305 kb on - strand at 3301.305 kb on - strand at 3301.467 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,298,578 + yhbS b3156 0.60 -0.7 3,298,578 + yhbS b3156 0.60 -0.6 3,298,591 - yhbS b3156 0.62 -0.5 3,298,635 - yhbS b3156 0.71 -2.6 3,298,635 - yhbS b3156 0.71 -3.0 3,298,649 - yhbS b3156 0.74 -1.6 3,298,724 + yhbS b3156 0.89 +0.0 3,298,737 + -2.8 3,298,737 + -0.1 3,298,858 + yhbT b3157 0.16 -0.6 3,298,864 + yhbT b3157 0.17 -2.2 3,298,951 - yhbT b3157 0.34 -1.2 3,299,033 - yhbT b3157 0.49 -0.3 3,299,033 - yhbT b3157 0.49 -2.1 3,299,115 + yhbT b3157 0.65 -0.5 3,299,123 - yhbT b3157 0.66 -2.2 3,299,123 - yhbT b3157 0.66 +0.3 3,299,220 - yhbT b3157 0.85 -0.3 3,299,399 - -0.7 3,299,399 - -1.4 3,299,515 + -0.6 3,299,515 + -1.7 3,299,550 - -2.2 3,299,626 + yhbU b3158 0.12 -0.1 3,299,785 - yhbU b3158 0.28 -2.6 3,299,785 - yhbU b3158 0.28 -0.2 3,299,809 - yhbU b3158 0.30 -1.5 3,299,857 + yhbU b3158 0.35 -2.7 3,299,860 - yhbU b3158 0.35 -1.5 3,299,968 + yhbU b3158 0.46 -1.9 3,299,968 + yhbU b3158 0.46 -0.2 3,299,968 + yhbU b3158 0.46 -1.0 3,299,981 - yhbU b3158 0.48 -2.3 3,300,162 + yhbU b3158 0.66 -1.4 3,300,216 - yhbU b3158 0.71 +0.3 3,300,216 - yhbU b3158 0.71 -1.6 3,300,225 - yhbU b3158 0.72 -1.9 3,300,240 - yhbU b3158 0.74 -3.0 3,300,349 - yhbU b3158 0.85 -0.6 3,300,349 - yhbU b3158 0.85 -2.1 3,300,388 + yhbU b3158 0.88 -0.0 3,300,396 - yhbU b3158 0.89 -0.3 3,300,396 - yhbU b3158 0.89 -1.4 3,300,396 - yhbU b3158 0.89 -0.5 3,300,396 - yhbU b3158 0.89 -0.7 3,300,396 - yhbU b3158 0.89 -2.4 3,300,451 + -2.8 3,300,451 + -0.3 3,300,451 + -0.3 3,300,451 + -1.8 3,300,476 + -1.3 3,300,532 + -2.9 3,300,540 - -1.4 3,300,541 - -0.7 3,300,543 - -0.2 3,300,543 - +0.2 3,300,545 + -1.1 3,300,630 - yhbV b3159 0.14 -2.1 3,300,632 - yhbV b3159 0.14 -0.2 3,300,729 + yhbV b3159 0.25 -2.0 3,300,742 - yhbV b3159 0.26 -3.0 3,300,742 - yhbV b3159 0.26 -2.2 3,300,742 - yhbV b3159 0.26 -0.7 3,300,761 + yhbV b3159 0.28 +0.3 3,300,861 + yhbV b3159 0.40 -1.3 3,300,917 - yhbV b3159 0.46 +1.0 3,300,922 - yhbV b3159 0.47 -1.4 3,300,957 + yhbV b3159 0.51 -0.1 3,300,993 + yhbV b3159 0.55 -1.8 3,300,993 + yhbV b3159 0.55 -2.0 3,301,040 - yhbV b3159 0.60 -1.6 3,301,094 - yhbV b3159 0.66 +0.3 3,301,094 - yhbV b3159 0.66 -1.0 3,301,100 + yhbV b3159 0.67 +0.5 3,301,100 + yhbV b3159 0.67 -0.3 3,301,230 + yhbV b3159 0.82 -0.7 3,301,230 + yhbV b3159 0.82 -1.3 3,301,245 + yhbV b3159 0.84 -1.8 3,301,245 + yhbV b3159 0.84 -3.4 3,301,286 - yhbV b3159 0.88 -1.4 3,301,286 - yhbV b3159 0.88 -1.1 3,301,305 - -0.7 3,301,305 - +2.0 3,301,467 + -0.9
Or see this region's nucleotide sequence