Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt uxaC and exuT are separated by 362 nucleotides exuT and exuR are separated by 129 nucleotides
b3092: uxaC - glucuronate isomerase (NCBI), at 3,241,351 to 3,242,763
uxaC
b3093: exuT - hexuronate transporter (NCBI), at 3,243,126 to 3,244,544
exuT
b3094: exuR - negative regulator of exu regulon, exuT, uxaAC, and uxuB (VIMSS), at 3,244,674 to 3,245,450
exuR
Position (kb)
3243
3244
3245 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3242.137 kb on + strand, within uxaC at 3242.167 kb on + strand, within uxaC at 3242.184 kb on - strand, within uxaC at 3242.184 kb on - strand, within uxaC at 3242.221 kb on - strand, within uxaC at 3242.221 kb on - strand, within uxaC at 3242.271 kb on + strand, within uxaC at 3242.333 kb on - strand, within uxaC at 3242.345 kb on + strand, within uxaC at 3242.345 kb on + strand, within uxaC at 3242.353 kb on - strand, within uxaC at 3242.353 kb on - strand, within uxaC at 3242.358 kb on - strand, within uxaC at 3242.709 kb on - strand at 3242.709 kb on - strand at 3242.747 kb on + strand at 3243.301 kb on - strand, within exuT at 3243.308 kb on + strand, within exuT at 3243.308 kb on + strand, within exuT at 3243.336 kb on - strand, within exuT at 3243.401 kb on + strand, within exuT at 3243.423 kb on - strand, within exuT at 3243.434 kb on - strand, within exuT at 3243.441 kb on + strand, within exuT at 3243.450 kb on - strand, within exuT at 3243.450 kb on - strand, within exuT at 3243.487 kb on + strand, within exuT at 3243.487 kb on + strand, within exuT at 3243.490 kb on - strand, within exuT at 3243.494 kb on + strand, within exuT at 3243.506 kb on + strand, within exuT at 3243.507 kb on - strand, within exuT at 3243.507 kb on - strand, within exuT at 3243.511 kb on + strand, within exuT at 3243.511 kb on + strand, within exuT at 3243.514 kb on - strand, within exuT at 3243.514 kb on - strand, within exuT at 3243.552 kb on - strand, within exuT at 3243.552 kb on - strand, within exuT at 3243.574 kb on - strand, within exuT at 3243.574 kb on - strand, within exuT at 3243.720 kb on - strand, within exuT at 3243.777 kb on - strand, within exuT at 3243.867 kb on - strand, within exuT at 3243.881 kb on + strand, within exuT at 3243.899 kb on - strand, within exuT at 3243.911 kb on + strand, within exuT at 3243.911 kb on + strand, within exuT at 3243.968 kb on + strand, within exuT at 3244.006 kb on + strand, within exuT at 3244.035 kb on - strand, within exuT at 3244.035 kb on - strand, within exuT at 3244.067 kb on + strand, within exuT at 3244.067 kb on + strand, within exuT at 3244.131 kb on - strand, within exuT at 3244.144 kb on + strand, within exuT at 3244.144 kb on + strand, within exuT at 3244.176 kb on + strand, within exuT at 3244.178 kb on + strand, within exuT at 3244.208 kb on + strand, within exuT at 3244.474 kb on - strand at 3244.474 kb on - strand at 3244.496 kb on + strand at 3244.505 kb on + strand at 3244.577 kb on - strand at 3244.577 kb on - strand at 3244.621 kb on - strand at 3244.621 kb on - strand at 3244.651 kb on + strand at 3244.664 kb on - strand at 3244.668 kb on - strand at 3244.689 kb on - strand at 3244.742 kb on - strand at 3244.860 kb on - strand, within exuR at 3245.058 kb on - strand, within exuR at 3245.058 kb on - strand, within exuR at 3245.086 kb on - strand, within exuR at 3245.086 kb on - strand, within exuR at 3245.098 kb on - strand, within exuR at 3245.168 kb on + strand, within exuR at 3245.168 kb on + strand, within exuR at 3245.183 kb on - strand, within exuR at 3245.200 kb on + strand, within exuR at 3245.217 kb on - strand, within exuR at 3245.299 kb on + strand, within exuR at 3245.299 kb on + strand, within exuR at 3245.376 kb on - strand at 3245.415 kb on + strand at 3245.420 kb on + strand at 3245.512 kb on + strand at 3245.514 kb on + strand at 3245.522 kb on - strand at 3245.532 kb on - strand at 3245.536 kb on + strand at 3245.536 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,242,137 + uxaC b3092 0.56 -0.9 3,242,167 + uxaC b3092 0.58 +1.0 3,242,184 - uxaC b3092 0.59 +0.5 3,242,184 - uxaC b3092 0.59 +1.4 3,242,221 - uxaC b3092 0.62 -0.3 3,242,221 - uxaC b3092 0.62 -2.8 3,242,271 + uxaC b3092 0.65 -0.2 3,242,333 - uxaC b3092 0.69 -0.1 3,242,345 + uxaC b3092 0.70 +0.8 3,242,345 + uxaC b3092 0.70 -1.9 3,242,353 - uxaC b3092 0.71 +0.6 3,242,353 - uxaC b3092 0.71 -1.3 3,242,358 - uxaC b3092 0.71 -0.2 3,242,709 - +0.7 3,242,709 - -0.6 3,242,747 + +2.1 3,243,301 - exuT b3093 0.12 +3.4 3,243,308 + exuT b3093 0.13 -0.9 3,243,308 + exuT b3093 0.13 +1.9 3,243,336 - exuT b3093 0.15 -0.3 3,243,401 + exuT b3093 0.19 -0.0 3,243,423 - exuT b3093 0.21 +0.2 3,243,434 - exuT b3093 0.22 -3.1 3,243,441 + exuT b3093 0.22 -1.5 3,243,450 - exuT b3093 0.23 -2.7 3,243,450 - exuT b3093 0.23 +0.7 3,243,487 + exuT b3093 0.25 +2.0 3,243,487 + exuT b3093 0.25 -3.0 3,243,490 - exuT b3093 0.26 +0.3 3,243,494 + exuT b3093 0.26 +0.2 3,243,506 + exuT b3093 0.27 -2.5 3,243,507 - exuT b3093 0.27 +1.2 3,243,507 - exuT b3093 0.27 +0.1 3,243,511 + exuT b3093 0.27 -1.3 3,243,511 + exuT b3093 0.27 +0.6 3,243,514 - exuT b3093 0.27 -1.6 3,243,514 - exuT b3093 0.27 -2.5 3,243,552 - exuT b3093 0.30 -1.6 3,243,552 - exuT b3093 0.30 -0.6 3,243,574 - exuT b3093 0.32 +0.7 3,243,574 - exuT b3093 0.32 +0.1 3,243,720 - exuT b3093 0.42 -0.7 3,243,777 - exuT b3093 0.46 +0.1 3,243,867 - exuT b3093 0.52 -2.2 3,243,881 + exuT b3093 0.53 +0.7 3,243,899 - exuT b3093 0.54 -0.9 3,243,911 + exuT b3093 0.55 -3.2 3,243,911 + exuT b3093 0.55 +1.4 3,243,968 + exuT b3093 0.59 +3.2 3,244,006 + exuT b3093 0.62 +0.8 3,244,035 - exuT b3093 0.64 -1.1 3,244,035 - exuT b3093 0.64 -0.8 3,244,067 + exuT b3093 0.66 +0.4 3,244,067 + exuT b3093 0.66 +1.6 3,244,131 - exuT b3093 0.71 -1.1 3,244,144 + exuT b3093 0.72 -1.4 3,244,144 + exuT b3093 0.72 -0.6 3,244,176 + exuT b3093 0.74 -0.4 3,244,178 + exuT b3093 0.74 -1.6 3,244,208 + exuT b3093 0.76 -0.4 3,244,474 - -0.8 3,244,474 - -0.3 3,244,496 + -0.2 3,244,505 + -1.1 3,244,577 - +0.1 3,244,577 - -1.5 3,244,621 - -0.8 3,244,621 - +0.2 3,244,651 + -0.2 3,244,664 - -2.0 3,244,668 - +0.1 3,244,689 - -1.9 3,244,742 - -1.6 3,244,860 - exuR b3094 0.24 +1.4 3,245,058 - exuR b3094 0.49 -1.8 3,245,058 - exuR b3094 0.49 -3.1 3,245,086 - exuR b3094 0.53 -0.8 3,245,086 - exuR b3094 0.53 -0.9 3,245,098 - exuR b3094 0.55 -0.8 3,245,168 + exuR b3094 0.64 -0.3 3,245,168 + exuR b3094 0.64 -1.5 3,245,183 - exuR b3094 0.66 -1.6 3,245,200 + exuR b3094 0.68 -2.9 3,245,217 - exuR b3094 0.70 -3.8 3,245,299 + exuR b3094 0.80 -2.9 3,245,299 + exuR b3094 0.80 -3.1 3,245,376 - -1.1 3,245,415 + +3.2 3,245,420 + +1.4 3,245,512 + +1.5 3,245,514 + +3.4 3,245,522 - -1.9 3,245,532 - -0.0 3,245,536 + +2.9 3,245,536 + +1.5
Or see this region's nucleotide sequence