Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hybF and hybE overlap by 8 nucleotides hybE and hybD overlap by 8 nucleotides hybD and hybC overlap by 1 nucleotides hybC and hybB overlap by 4 nucleotides
b2991: hybF - protein involved with the maturation of hydrogenases 1 and 2 (NCBI), at 3,137,999 to 3,138,340
hybF
b2992: hybE - hydrogenase 2-specific chaperone (NCBI), at 3,138,333 to 3,138,821
hybE
b2993: hybD - predicted maturation element for hydrogenase 2 (NCBI), at 3,138,814 to 3,139,308
hybD
b2994: hybC - hydrogenase 2, large subunit (NCBI), at 3,139,308 to 3,141,011
hybC
b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186
hybB
Position (kb)
3139
3140
3141
3142 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3138.363 kb on + strand at 3138.474 kb on + strand, within hybE at 3138.474 kb on + strand, within hybE at 3138.640 kb on + strand, within hybE at 3138.640 kb on + strand, within hybE at 3138.649 kb on + strand, within hybE at 3138.658 kb on - strand, within hybE at 3138.707 kb on + strand, within hybE at 3138.715 kb on - strand, within hybE at 3138.839 kb on - strand at 3138.839 kb on - strand at 3138.928 kb on + strand, within hybD at 3139.075 kb on - strand, within hybD at 3139.075 kb on - strand, within hybD at 3139.157 kb on + strand, within hybD at 3139.227 kb on + strand, within hybD at 3139.242 kb on - strand, within hybD at 3139.245 kb on + strand, within hybD at 3139.288 kb on + strand at 3139.308 kb on + strand at 3139.371 kb on - strand at 3139.371 kb on - strand at 3139.379 kb on + strand at 3139.379 kb on + strand at 3139.379 kb on + strand at 3139.396 kb on + strand at 3139.443 kb on - strand at 3139.538 kb on - strand, within hybC at 3139.613 kb on - strand, within hybC at 3139.676 kb on + strand, within hybC at 3139.683 kb on + strand, within hybC at 3139.701 kb on + strand, within hybC at 3139.716 kb on + strand, within hybC at 3139.763 kb on + strand, within hybC at 3139.832 kb on - strand, within hybC at 3139.864 kb on + strand, within hybC at 3140.001 kb on - strand, within hybC at 3140.013 kb on - strand, within hybC at 3140.013 kb on - strand, within hybC at 3140.129 kb on + strand, within hybC at 3140.159 kb on + strand, within hybC at 3140.167 kb on - strand, within hybC at 3140.167 kb on - strand, within hybC at 3140.227 kb on + strand at 3140.227 kb on + strand, within hybC at 3140.235 kb on + strand, within hybC at 3140.284 kb on + strand, within hybC at 3140.320 kb on - strand, within hybC at 3140.361 kb on + strand, within hybC at 3140.391 kb on - strand, within hybC at 3140.418 kb on + strand, within hybC at 3140.418 kb on + strand, within hybC at 3140.525 kb on - strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.583 kb on + strand, within hybC at 3140.591 kb on - strand, within hybC at 3140.591 kb on - strand, within hybC at 3140.641 kb on - strand, within hybC at 3140.657 kb on + strand, within hybC at 3140.657 kb on + strand, within hybC at 3140.823 kb on - strand, within hybC at 3140.838 kb on + strand, within hybC at 3140.838 kb on + strand, within hybC at 3140.852 kb on - strand at 3140.885 kb on + strand at 3141.123 kb on + strand at 3141.192 kb on - strand, within hybB at 3141.215 kb on + strand, within hybB at 3141.215 kb on + strand, within hybB at 3141.223 kb on - strand, within hybB at 3141.240 kb on - strand, within hybB at 3141.240 kb on - strand, within hybB at 3141.286 kb on - strand, within hybB at 3141.341 kb on - strand, within hybB at 3141.341 kb on - strand, within hybB at 3141.472 kb on + strand, within hybB at 3141.486 kb on + strand, within hybB at 3141.554 kb on - strand, within hybB at 3141.632 kb on + strand, within hybB at 3141.642 kb on + strand, within hybB at 3141.642 kb on + strand, within hybB at 3141.657 kb on - strand, within hybB at 3141.676 kb on - strand, within hybB at 3141.733 kb on - strand, within hybB at 3141.738 kb on - strand, within hybB at 3141.738 kb on - strand, within hybB at 3141.751 kb on - strand, within hybB at 3141.751 kb on - strand, within hybB at 3141.779 kb on - strand, within hybB at 3141.804 kb on - strand, within hybB at 3141.808 kb on + strand, within hybB at 3141.843 kb on + strand, within hybB at 3141.851 kb on - strand, within hybB at 3141.888 kb on + strand, within hybB at 3141.901 kb on - strand, within hybB at 3141.901 kb on - strand, within hybB at 3141.913 kb on - strand, within hybB at 3141.955 kb on - strand, within hybB at 3141.955 kb on - strand, within hybB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,138,363 + +0.7 3,138,474 + hybE b2992 0.29 -0.7 3,138,474 + hybE b2992 0.29 -1.4 3,138,640 + hybE b2992 0.63 -0.9 3,138,640 + hybE b2992 0.63 -1.6 3,138,649 + hybE b2992 0.65 -0.6 3,138,658 - hybE b2992 0.66 -2.3 3,138,707 + hybE b2992 0.76 -0.7 3,138,715 - hybE b2992 0.78 -0.3 3,138,839 - +0.3 3,138,839 - -1.1 3,138,928 + hybD b2993 0.23 -1.8 3,139,075 - hybD b2993 0.53 -0.7 3,139,075 - hybD b2993 0.53 +0.7 3,139,157 + hybD b2993 0.69 -1.6 3,139,227 + hybD b2993 0.83 +0.5 3,139,242 - hybD b2993 0.86 -1.8 3,139,245 + hybD b2993 0.87 -1.7 3,139,288 + +0.8 3,139,308 + +0.5 3,139,371 - -2.4 3,139,371 - -0.8 3,139,379 + -2.4 3,139,379 + -0.4 3,139,379 + -0.6 3,139,396 + -0.2 3,139,443 - -0.9 3,139,538 - hybC b2994 0.13 -1.6 3,139,613 - hybC b2994 0.18 -2.9 3,139,676 + hybC b2994 0.22 -0.8 3,139,683 + hybC b2994 0.22 -1.5 3,139,701 + hybC b2994 0.23 -2.9 3,139,716 + hybC b2994 0.24 -0.7 3,139,763 + hybC b2994 0.27 -3.0 3,139,832 - hybC b2994 0.31 -0.9 3,139,864 + hybC b2994 0.33 -1.1 3,140,001 - hybC b2994 0.41 -2.4 3,140,013 - hybC b2994 0.41 -1.1 3,140,013 - hybC b2994 0.41 -0.1 3,140,129 + hybC b2994 0.48 -1.1 3,140,159 + hybC b2994 0.50 -2.3 3,140,167 - hybC b2994 0.50 -1.1 3,140,167 - hybC b2994 0.50 -2.4 3,140,227 + +0.2 3,140,227 + hybC b2994 0.54 +0.2 3,140,235 + hybC b2994 0.54 -3.3 3,140,284 + hybC b2994 0.57 +0.2 3,140,320 - hybC b2994 0.59 -0.7 3,140,361 + hybC b2994 0.62 -2.1 3,140,391 - hybC b2994 0.64 -0.5 3,140,418 + hybC b2994 0.65 -0.0 3,140,418 + hybC b2994 0.65 +0.0 3,140,525 - hybC b2994 0.71 -0.2 3,140,583 + hybC b2994 0.75 +0.1 3,140,583 + hybC b2994 0.75 -1.7 3,140,583 + hybC b2994 0.75 -2.2 3,140,583 + hybC b2994 0.75 -1.8 3,140,591 - hybC b2994 0.75 -1.4 3,140,591 - hybC b2994 0.75 -1.6 3,140,641 - hybC b2994 0.78 -3.3 3,140,657 + hybC b2994 0.79 -1.9 3,140,657 + hybC b2994 0.79 -1.8 3,140,823 - hybC b2994 0.89 -3.2 3,140,838 + hybC b2994 0.90 -1.8 3,140,838 + hybC b2994 0.90 -0.6 3,140,852 - -0.8 3,140,885 + -0.6 3,141,123 + -2.4 3,141,192 - hybB b2995 0.16 -1.1 3,141,215 + hybB b2995 0.18 -2.0 3,141,215 + hybB b2995 0.18 -0.3 3,141,223 - hybB b2995 0.18 -2.5 3,141,240 - hybB b2995 0.20 -1.0 3,141,240 - hybB b2995 0.20 -1.0 3,141,286 - hybB b2995 0.24 -0.0 3,141,341 - hybB b2995 0.28 -1.1 3,141,341 - hybB b2995 0.28 +0.9 3,141,472 + hybB b2995 0.39 -1.1 3,141,486 + hybB b2995 0.41 -0.4 3,141,554 - hybB b2995 0.46 +0.2 3,141,632 + hybB b2995 0.53 -1.0 3,141,642 + hybB b2995 0.54 -2.3 3,141,642 + hybB b2995 0.54 -0.8 3,141,657 - hybB b2995 0.55 -0.3 3,141,676 - hybB b2995 0.57 -1.8 3,141,733 - hybB b2995 0.61 -0.2 3,141,738 - hybB b2995 0.62 +0.0 3,141,738 - hybB b2995 0.62 +0.5 3,141,751 - hybB b2995 0.63 -1.4 3,141,751 - hybB b2995 0.63 +0.1 3,141,779 - hybB b2995 0.65 -0.6 3,141,804 - hybB b2995 0.68 -3.1 3,141,808 + hybB b2995 0.68 -4.0 3,141,843 + hybB b2995 0.71 +0.3 3,141,851 - hybB b2995 0.72 -1.0 3,141,888 + hybB b2995 0.75 -1.4 3,141,901 - hybB b2995 0.76 -1.0 3,141,901 - hybB b2995 0.76 -2.1 3,141,913 - hybB b2995 0.77 -0.7 3,141,955 - hybB b2995 0.80 -0.8 3,141,955 - hybB b2995 0.80 -0.7
Or see this region's nucleotide sequence