Strain Fitness in Escherichia coli BW25113 around b2837

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaas and rygA are separated by 87 nucleotidesrygA and omrB are separated by 114 nucleotidesomrB and galR are separated by 213 nucleotidesgalR and lysA are separated by 6 nucleotides b2836: aas - 2-acyl-glycerophospho-ethanolamine acyltransferase (NCBI), at 2,971,877 to 2,974,036 aas b4444: rygA - unknown RNA (NCBI), at 2,974,124 to 2,974,211 rygA b4445: omrB - sRNA down regulates OM proteins; positively regulated by OmpR/EnvZ; binds Hfq (RefSeq), at 2,974,326 to 2,974,407 omrB b2837: galR - DNA-binding transcriptional repressor (NCBI), at 2,974,621 to 2,975,652 galR b2838: lysA - diaminopimelate decarboxylase, PLP-binding (NCBI), at 2,975,659 to 2,976,921 lysA Position (kb) 2974 2975 2976Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2973.699 kb on - strand, within aasat 2973.741 kb on + strand, within aasat 2973.754 kb on + strand, within aasat 2973.831 kb on - strandat 2973.831 kb on - strandat 2973.842 kb on - strandat 2973.894 kb on - strandat 2973.916 kb on + strandat 2973.918 kb on + strandat 2973.974 kb on - strandat 2973.995 kb on + strandat 2974.053 kb on - strandat 2974.055 kb on + strandat 2974.055 kb on + strandat 2974.090 kb on + strandat 2974.090 kb on + strandat 2974.097 kb on - strandat 2974.148 kb on + strand, within rygAat 2974.161 kb on - strand, within rygAat 2974.163 kb on - strand, within rygAat 2974.163 kb on - strand, within rygAat 2974.168 kb on - strand, within rygAat 2974.174 kb on - strand, within rygAat 2974.183 kb on - strand, within rygAat 2974.340 kb on + strand, within omrBat 2974.353 kb on - strand, within omrBat 2974.363 kb on - strand, within omrBat 2974.381 kb on - strand, within omrBat 2974.429 kb on - strandat 2974.581 kb on - strandat 2974.635 kb on + strandat 2974.635 kb on + strandat 2974.636 kb on - strandat 2974.636 kb on - strandat 2974.684 kb on + strandat 2974.684 kb on + strandat 2974.723 kb on + strandat 2974.733 kb on + strand, within galRat 2974.733 kb on + strand, within galRat 2974.741 kb on - strand, within galRat 2974.741 kb on - strand, within galRat 2974.751 kb on + strand, within galRat 2974.793 kb on + strand, within galRat 2974.793 kb on + strand, within galRat 2975.062 kb on - strand, within galRat 2975.089 kb on + strand, within galRat 2975.111 kb on - strand, within galRat 2975.111 kb on - strand, within galRat 2975.152 kb on - strand, within galRat 2975.168 kb on + strand, within galRat 2975.176 kb on - strand, within galRat 2975.348 kb on + strand, within galRat 2975.354 kb on - strand, within galRat 2975.357 kb on + strand, within galRat 2975.399 kb on + strand, within galRat 2975.399 kb on + strand, within galRat 2975.406 kb on - strand, within galRat 2975.406 kb on - strand, within galRat 2975.407 kb on - strand, within galRat 2975.457 kb on + strand, within galRat 2975.457 kb on + strand, within galRat 2975.478 kb on - strand, within galRat 2975.516 kb on - strand, within galRat 2975.627 kb on - strandat 2975.794 kb on - strand, within lysAat 2975.794 kb on - strand, within lysAat 2975.806 kb on - strand, within lysAat 2975.816 kb on - strand, within lysAat 2975.871 kb on + strand, within lysAat 2975.883 kb on - strand, within lysAat 2975.917 kb on + strand, within lysAat 2975.984 kb on + strand, within lysAat 2976.036 kb on - strand, within lysAat 2976.036 kb on - strand, within lysAat 2976.041 kb on - strand, within lysAat 2976.216 kb on - strand, within lysAat 2976.302 kb on + strand, within lysAat 2976.305 kb on + strand, within lysAat 2976.305 kb on + strand, within lysAat 2976.305 kb on - strand, within lysAat 2976.305 kb on - strand, within lysAat 2976.313 kb on - strand, within lysAat 2976.319 kb on - strand, within lysAat 2976.345 kb on - strand, within lysAat 2976.397 kb on - strand, within lysAat 2976.401 kb on + strand, within lysAat 2976.420 kb on + strand, within lysAat 2976.424 kb on - strand, within lysAat 2976.424 kb on - strand, within lysAat 2976.428 kb on - strand, within lysAat 2976.428 kb on - strand, within lysAat 2976.479 kb on - strand, within lysAat 2976.494 kb on + strand, within lysAat 2976.501 kb on + strand, within lysAat 2976.501 kb on + strand, within lysAat 2976.532 kb on - strand, within lysAat 2976.532 kb on - strand, within lysAat 2976.554 kb on - strand, within lysA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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2,973,699 - aas b2836 0.84 +0.2
2,973,741 + aas b2836 0.86 +0.7
2,973,754 + aas b2836 0.87 +1.8
2,973,831 - -0.2
2,973,831 - -0.2
2,973,842 - -0.5
2,973,894 - +0.1
2,973,916 + -0.4
2,973,918 + -2.0
2,973,974 - +0.6
2,973,995 + -0.9
2,974,053 - -0.6
2,974,055 + -1.4
2,974,055 + +1.2
2,974,090 + +0.6
2,974,090 + +0.2
2,974,097 - -1.7
2,974,148 + rygA b4444 0.27 +0.7
2,974,161 - rygA b4444 0.42 +0.1
2,974,163 - rygA b4444 0.44 +1.4
2,974,163 - rygA b4444 0.44 -1.6
2,974,168 - rygA b4444 0.50 +0.3
2,974,174 - rygA b4444 0.57 -0.7
2,974,183 - rygA b4444 0.67 -0.3
2,974,340 + omrB b4445 0.17 -0.0
2,974,353 - omrB b4445 0.33 -2.4
2,974,363 - omrB b4445 0.45 -0.8
2,974,381 - omrB b4445 0.67 -2.1
2,974,429 - -0.3
2,974,581 - +0.5
2,974,635 + -1.4
2,974,635 + +0.5
2,974,636 - -2.0
2,974,636 - -1.0
2,974,684 + -3.3
2,974,684 + -0.8
2,974,723 + +0.3
2,974,733 + galR b2837 0.11 +1.2
2,974,733 + galR b2837 0.11 -1.3
2,974,741 - galR b2837 0.12 -2.0
2,974,741 - galR b2837 0.12 -0.9
2,974,751 + galR b2837 0.13 -0.7
2,974,793 + galR b2837 0.17 -1.4
2,974,793 + galR b2837 0.17 +0.4
2,975,062 - galR b2837 0.43 -2.5
2,975,089 + galR b2837 0.45 +0.9
2,975,111 - galR b2837 0.47 -0.7
2,975,111 - galR b2837 0.47 -1.1
2,975,152 - galR b2837 0.51 -0.1
2,975,168 + galR b2837 0.53 -1.0
2,975,176 - galR b2837 0.54 -1.0
2,975,348 + galR b2837 0.70 -0.8
2,975,354 - galR b2837 0.71 +0.2
2,975,357 + galR b2837 0.71 +0.3
2,975,399 + galR b2837 0.75 -1.3
2,975,399 + galR b2837 0.75 -1.7
2,975,406 - galR b2837 0.76 -2.0
2,975,406 - galR b2837 0.76 +0.5
2,975,407 - galR b2837 0.76 -1.6
2,975,457 + galR b2837 0.81 -0.2
2,975,457 + galR b2837 0.81 -0.7
2,975,478 - galR b2837 0.83 +1.5
2,975,516 - galR b2837 0.87 +0.2
2,975,627 - -2.1
2,975,794 - lysA b2838 0.11 +1.9
2,975,794 - lysA b2838 0.11 -0.4
2,975,806 - lysA b2838 0.12 -0.1
2,975,816 - lysA b2838 0.12 +0.4
2,975,871 + lysA b2838 0.17 -0.1
2,975,883 - lysA b2838 0.18 -1.4
2,975,917 + lysA b2838 0.20 +0.2
2,975,984 + lysA b2838 0.26 -0.1
2,976,036 - lysA b2838 0.30 -3.2
2,976,036 - lysA b2838 0.30 -0.1
2,976,041 - lysA b2838 0.30 -0.3
2,976,216 - lysA b2838 0.44 -1.9
2,976,302 + lysA b2838 0.51 +1.2
2,976,305 + lysA b2838 0.51 +0.9
2,976,305 + lysA b2838 0.51 +0.6
2,976,305 - lysA b2838 0.51 -0.1
2,976,305 - lysA b2838 0.51 -0.2
2,976,313 - lysA b2838 0.52 +0.2
2,976,319 - lysA b2838 0.52 -3.1
2,976,345 - lysA b2838 0.54 +0.8
2,976,397 - lysA b2838 0.58 +0.4
2,976,401 + lysA b2838 0.59 +1.0
2,976,420 + lysA b2838 0.60 +0.6
2,976,424 - lysA b2838 0.61 +0.2
2,976,424 - lysA b2838 0.61 -1.8
2,976,428 - lysA b2838 0.61 -1.8
2,976,428 - lysA b2838 0.61 -0.6
2,976,479 - lysA b2838 0.65 +1.2
2,976,494 + lysA b2838 0.66 +1.0
2,976,501 + lysA b2838 0.67 +0.2
2,976,501 + lysA b2838 0.67 -0.7
2,976,532 - lysA b2838 0.69 +1.6
2,976,532 - lysA b2838 0.69 +0.3
2,976,554 - lysA b2838 0.71 +0.9

Or see this region's nucleotide sequence