Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygdL and mltA are separated by 238 nucleotides mltA and metZ are separated by 208 nucleotides metZ and metW are separated by 33 nucleotides metW and metV are separated by 33 nucleotides metV and b2817 are separated by 73 nucleotides
b2812: ygdL - hypothetical protein (NCBI), at 2,943,058 to 2,943,864
ygdL
b2813: mltA - membrane-bound lytic murein transglycosylase A (NCBI), at 2,944,103 to 2,945,200
mltA
b2814: metZ - tRNA-Met (NCBI), at 2,945,409 to 2,945,485
metZ
b2815: metW - tRNA-Met (NCBI), at 2,945,519 to 2,945,595
metW
b2816: metV - tRNA-Met (NCBI), at 2,945,629 to 2,945,705
metV
b2817: b2817 - putative amidase (VIMSS), at 2,945,779 to 2,947,032
b2817
Position (kb)
2944
2945
2946 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2943.138 kb on - strand at 2943.208 kb on + strand, within ygdL at 2943.208 kb on + strand, within ygdL at 2943.240 kb on - strand, within ygdL at 2943.453 kb on + strand, within ygdL at 2943.473 kb on - strand, within ygdL at 2943.478 kb on - strand, within ygdL at 2943.509 kb on + strand, within ygdL at 2943.509 kb on + strand, within ygdL at 2943.517 kb on - strand, within ygdL at 2943.517 kb on - strand, within ygdL at 2943.517 kb on - strand, within ygdL at 2943.532 kb on - strand, within ygdL at 2943.563 kb on - strand, within ygdL at 2943.580 kb on - strand, within ygdL at 2943.580 kb on - strand, within ygdL at 2943.636 kb on - strand, within ygdL at 2943.754 kb on - strand, within ygdL at 2943.756 kb on + strand, within ygdL at 2943.756 kb on + strand, within ygdL at 2943.769 kb on - strand, within ygdL at 2943.805 kb on + strand at 2943.890 kb on + strand at 2943.890 kb on + strand at 2943.904 kb on - strand at 2943.904 kb on - strand at 2943.910 kb on - strand at 2943.910 kb on - strand at 2944.009 kb on + strand at 2944.012 kb on + strand at 2944.064 kb on - strand at 2944.064 kb on - strand at 2944.076 kb on - strand at 2944.076 kb on - strand at 2944.155 kb on + strand at 2944.155 kb on + strand at 2944.335 kb on + strand, within mltA at 2944.335 kb on + strand, within mltA at 2944.338 kb on - strand, within mltA at 2944.340 kb on - strand, within mltA at 2944.357 kb on + strand, within mltA at 2944.357 kb on + strand, within mltA at 2944.366 kb on - strand, within mltA at 2944.366 kb on - strand, within mltA at 2944.372 kb on - strand, within mltA at 2944.372 kb on - strand, within mltA at 2944.433 kb on + strand, within mltA at 2944.433 kb on + strand, within mltA at 2944.455 kb on + strand, within mltA at 2944.485 kb on - strand, within mltA at 2944.485 kb on - strand, within mltA at 2944.553 kb on + strand, within mltA at 2944.553 kb on + strand, within mltA at 2944.561 kb on - strand, within mltA at 2944.564 kb on + strand, within mltA at 2944.566 kb on - strand, within mltA at 2944.569 kb on + strand, within mltA at 2944.652 kb on + strand, within mltA at 2944.736 kb on - strand, within mltA at 2944.860 kb on + strand, within mltA at 2944.860 kb on + strand, within mltA at 2944.883 kb on - strand, within mltA at 2944.883 kb on - strand, within mltA at 2944.943 kb on + strand, within mltA at 2944.943 kb on + strand, within mltA at 2944.943 kb on + strand, within mltA at 2945.034 kb on + strand, within mltA at 2945.034 kb on + strand, within mltA at 2945.094 kb on - strand at 2945.105 kb on + strand at 2945.105 kb on + strand at 2945.124 kb on + strand at 2945.184 kb on + strand at 2945.272 kb on + strand at 2945.423 kb on + strand, within metZ at 2945.508 kb on - strand at 2946.029 kb on + strand, within b2817 at 2946.029 kb on + strand, within b2817 at 2946.068 kb on - strand, within b2817 at 2946.068 kb on - strand, within b2817 at 2946.086 kb on + strand, within b2817 at 2946.094 kb on + strand, within b2817 at 2946.164 kb on + strand, within b2817 at 2946.172 kb on - strand, within b2817
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 2,943,138 - -2.4 2,943,208 + ygdL b2812 0.19 +1.0 2,943,208 + ygdL b2812 0.19 -0.4 2,943,240 - ygdL b2812 0.23 +0.2 2,943,453 + ygdL b2812 0.49 +0.5 2,943,473 - ygdL b2812 0.51 -1.5 2,943,478 - ygdL b2812 0.52 +0.9 2,943,509 + ygdL b2812 0.56 -2.3 2,943,509 + ygdL b2812 0.56 +0.4 2,943,517 - ygdL b2812 0.57 -3.0 2,943,517 - ygdL b2812 0.57 -1.2 2,943,517 - ygdL b2812 0.57 +0.3 2,943,532 - ygdL b2812 0.59 -0.8 2,943,563 - ygdL b2812 0.63 -1.6 2,943,580 - ygdL b2812 0.65 -0.1 2,943,580 - ygdL b2812 0.65 +0.4 2,943,636 - ygdL b2812 0.72 -0.6 2,943,754 - ygdL b2812 0.86 +0.8 2,943,756 + ygdL b2812 0.86 -1.8 2,943,756 + ygdL b2812 0.86 +1.3 2,943,769 - ygdL b2812 0.88 -2.3 2,943,805 + -0.4 2,943,890 + +0.2 2,943,890 + -1.8 2,943,904 - +0.6 2,943,904 - +0.4 2,943,910 - +1.0 2,943,910 - -0.9 2,944,009 + -0.1 2,944,012 + +0.2 2,944,064 - -0.1 2,944,064 - +0.7 2,944,076 - -0.6 2,944,076 - -1.0 2,944,155 + -3.0 2,944,155 + -1.3 2,944,335 + mltA b2813 0.21 -2.0 2,944,335 + mltA b2813 0.21 -1.4 2,944,338 - mltA b2813 0.21 -0.8 2,944,340 - mltA b2813 0.22 -2.9 2,944,357 + mltA b2813 0.23 -0.4 2,944,357 + mltA b2813 0.23 -3.2 2,944,366 - mltA b2813 0.24 -3.5 2,944,366 - mltA b2813 0.24 -2.4 2,944,372 - mltA b2813 0.24 -4.5 2,944,372 - mltA b2813 0.24 -3.2 2,944,433 + mltA b2813 0.30 -1.1 2,944,433 + mltA b2813 0.30 -2.3 2,944,455 + mltA b2813 0.32 -0.1 2,944,485 - mltA b2813 0.35 -2.2 2,944,485 - mltA b2813 0.35 -0.3 2,944,553 + mltA b2813 0.41 -4.0 2,944,553 + mltA b2813 0.41 -2.4 2,944,561 - mltA b2813 0.42 -3.0 2,944,564 + mltA b2813 0.42 -2.5 2,944,566 - mltA b2813 0.42 -1.6 2,944,569 + mltA b2813 0.42 -2.3 2,944,652 + mltA b2813 0.50 -3.0 2,944,736 - mltA b2813 0.58 -1.9 2,944,860 + mltA b2813 0.69 -1.6 2,944,860 + mltA b2813 0.69 -1.1 2,944,883 - mltA b2813 0.71 -1.6 2,944,883 - mltA b2813 0.71 -2.5 2,944,943 + mltA b2813 0.77 -2.7 2,944,943 + mltA b2813 0.77 -1.1 2,944,943 + mltA b2813 0.77 -3.1 2,945,034 + mltA b2813 0.85 -2.9 2,945,034 + mltA b2813 0.85 -3.1 2,945,094 - -2.0 2,945,105 + -2.5 2,945,105 + -2.0 2,945,124 + -2.4 2,945,184 + -3.2 2,945,272 + -0.8 2,945,423 + metZ b2814 0.18 -0.0 2,945,508 - -0.8 2,946,029 + b2817 b2817 0.20 -1.8 2,946,029 + b2817 b2817 0.20 -0.5 2,946,068 - b2817 b2817 0.23 -0.4 2,946,068 - b2817 b2817 0.23 -1.0 2,946,086 + b2817 b2817 0.24 +0.4 2,946,094 + b2817 b2817 0.25 -0.3 2,946,164 + b2817 b2817 0.31 +0.8 2,946,172 - b2817 b2817 0.31 -0.8
Or see this region's nucleotide sequence