Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypB and hypC overlap by 10 nucleotides hypC and hypD overlap by 1 nucleotides hypD and hypE overlap by 4 nucleotides
b2727: hypB - GTP hydrolase involved in nickel liganding into hydrogenases (NCBI), at 2,849,023 to 2,849,895
hypB
b2728: hypC - protein required for maturation of hydrogenases 1 and 3 (NCBI), at 2,849,886 to 2,850,158
hypC
b2729: hypD - protein required for maturation of hydrogenases (NCBI), at 2,850,158 to 2,851,279
hypD
b2730: hypE - carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (RefSeq), at 2,851,276 to 2,852,286
hypE
Position (kb)
2850
2851
2852 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2849.290 kb on - strand, within hypB at 2849.290 kb on - strand, within hypB at 2849.345 kb on + strand, within hypB at 2849.390 kb on - strand, within hypB at 2849.430 kb on + strand, within hypB at 2849.430 kb on + strand, within hypB at 2849.486 kb on + strand, within hypB at 2849.486 kb on + strand, within hypB at 2849.494 kb on + strand, within hypB at 2849.507 kb on - strand, within hypB at 2849.581 kb on - strand, within hypB at 2849.748 kb on + strand, within hypB at 2849.884 kb on - strand at 2849.917 kb on + strand, within hypC at 2849.917 kb on + strand, within hypC at 2849.917 kb on - strand, within hypC at 2849.917 kb on - strand, within hypC at 2850.031 kb on - strand, within hypC at 2850.085 kb on + strand, within hypC at 2850.093 kb on - strand, within hypC at 2850.093 kb on - strand, within hypC at 2850.145 kb on - strand at 2850.170 kb on + strand at 2850.170 kb on + strand at 2850.222 kb on + strand at 2850.236 kb on + strand at 2850.246 kb on - strand at 2850.297 kb on - strand, within hypD at 2850.297 kb on - strand, within hypD at 2850.350 kb on - strand, within hypD at 2850.350 kb on - strand, within hypD at 2850.354 kb on + strand, within hypD at 2850.369 kb on - strand, within hypD at 2850.369 kb on - strand, within hypD at 2850.452 kb on + strand, within hypD at 2850.522 kb on - strand, within hypD at 2850.522 kb on - strand, within hypD at 2850.616 kb on - strand, within hypD at 2850.624 kb on - strand, within hypD at 2850.681 kb on + strand, within hypD at 2850.691 kb on - strand, within hypD at 2850.913 kb on - strand, within hypD at 2850.913 kb on - strand, within hypD at 2850.955 kb on - strand, within hypD at 2850.971 kb on + strand, within hypD at 2851.008 kb on + strand, within hypD at 2851.008 kb on + strand, within hypD at 2851.099 kb on + strand, within hypD at 2851.132 kb on + strand, within hypD at 2851.141 kb on - strand, within hypD at 2851.180 kb on + strand at 2851.180 kb on + strand at 2851.254 kb on - strand at 2851.318 kb on + strand at 2851.359 kb on - strand at 2851.359 kb on - strand at 2851.420 kb on - strand, within hypE at 2851.438 kb on - strand, within hypE at 2851.468 kb on + strand, within hypE at 2851.480 kb on + strand, within hypE at 2851.480 kb on + strand, within hypE at 2851.524 kb on - strand, within hypE at 2851.538 kb on + strand, within hypE at 2851.538 kb on + strand, within hypE at 2851.831 kb on - strand, within hypE at 2851.874 kb on + strand, within hypE at 2851.892 kb on - strand, within hypE at 2851.903 kb on + strand, within hypE at 2851.940 kb on - strand, within hypE at 2852.009 kb on - strand, within hypE at 2852.024 kb on + strand, within hypE at 2852.024 kb on + strand, within hypE at 2852.046 kb on - strand, within hypE at 2852.046 kb on - strand, within hypE at 2852.108 kb on - strand, within hypE at 2852.108 kb on - strand, within hypE at 2852.199 kb on - strand at 2852.199 kb on - strand at 2852.234 kb on - strand at 2852.234 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 2,849,290 - hypB b2727 0.31 -3.0 2,849,290 - hypB b2727 0.31 -1.2 2,849,345 + hypB b2727 0.37 -1.0 2,849,390 - hypB b2727 0.42 -2.2 2,849,430 + hypB b2727 0.47 -0.5 2,849,430 + hypB b2727 0.47 -0.1 2,849,486 + hypB b2727 0.53 -1.7 2,849,486 + hypB b2727 0.53 -2.3 2,849,494 + hypB b2727 0.54 -1.7 2,849,507 - hypB b2727 0.55 -1.9 2,849,581 - hypB b2727 0.64 -2.1 2,849,748 + hypB b2727 0.83 -1.3 2,849,884 - -3.8 2,849,917 + hypC b2728 0.11 -1.8 2,849,917 + hypC b2728 0.11 -0.7 2,849,917 - hypC b2728 0.11 -2.7 2,849,917 - hypC b2728 0.11 -1.8 2,850,031 - hypC b2728 0.53 -1.3 2,850,085 + hypC b2728 0.73 -0.5 2,850,093 - hypC b2728 0.76 -0.8 2,850,093 - hypC b2728 0.76 -0.4 2,850,145 - +0.3 2,850,170 + -2.1 2,850,170 + -0.3 2,850,222 + -1.6 2,850,236 + -0.9 2,850,246 - -2.1 2,850,297 - hypD b2729 0.12 -1.9 2,850,297 - hypD b2729 0.12 -1.5 2,850,350 - hypD b2729 0.17 -3.1 2,850,350 - hypD b2729 0.17 +0.2 2,850,354 + hypD b2729 0.17 -0.7 2,850,369 - hypD b2729 0.19 +0.7 2,850,369 - hypD b2729 0.19 -0.7 2,850,452 + hypD b2729 0.26 -1.1 2,850,522 - hypD b2729 0.32 -1.0 2,850,522 - hypD b2729 0.32 -0.9 2,850,616 - hypD b2729 0.41 -0.7 2,850,624 - hypD b2729 0.42 -1.5 2,850,681 + hypD b2729 0.47 -1.2 2,850,691 - hypD b2729 0.48 -2.0 2,850,913 - hypD b2729 0.67 -1.5 2,850,913 - hypD b2729 0.67 -2.2 2,850,955 - hypD b2729 0.71 -1.8 2,850,971 + hypD b2729 0.72 -1.2 2,851,008 + hypD b2729 0.76 -0.1 2,851,008 + hypD b2729 0.76 -2.8 2,851,099 + hypD b2729 0.84 -2.4 2,851,132 + hypD b2729 0.87 -0.8 2,851,141 - hypD b2729 0.88 -0.8 2,851,180 + -0.9 2,851,180 + -0.9 2,851,254 - +0.4 2,851,318 + +0.5 2,851,359 - -2.8 2,851,359 - +1.1 2,851,420 - hypE b2730 0.14 -2.2 2,851,438 - hypE b2730 0.16 +0.1 2,851,468 + hypE b2730 0.19 -3.5 2,851,480 + hypE b2730 0.20 -2.6 2,851,480 + hypE b2730 0.20 -1.6 2,851,524 - hypE b2730 0.25 -2.1 2,851,538 + hypE b2730 0.26 -1.7 2,851,538 + hypE b2730 0.26 -1.5 2,851,831 - hypE b2730 0.55 +0.1 2,851,874 + hypE b2730 0.59 -1.8 2,851,892 - hypE b2730 0.61 -0.4 2,851,903 + hypE b2730 0.62 -2.3 2,851,940 - hypE b2730 0.66 -0.9 2,852,009 - hypE b2730 0.73 -1.8 2,852,024 + hypE b2730 0.74 -1.1 2,852,024 + hypE b2730 0.74 -0.9 2,852,046 - hypE b2730 0.76 -0.7 2,852,046 - hypE b2730 0.76 -0.7 2,852,108 - hypE b2730 0.82 -2.1 2,852,108 - hypE b2730 0.82 -2.1 2,852,199 - -1.3 2,852,199 - +0.3 2,852,234 - +1.1 2,852,234 - -0.9
Or see this region's nucleotide sequence