Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recA and ygaD are separated by 79 nucleotides ygaD and mltB are separated by 144 nucleotides mltB and srlA are separated by 255 nucleotides srlA and srlE overlap by 4 nucleotides
b2699: recA - recombinase A (NCBI), at 2,820,730 to 2,821,791
recA
b2700: ygaD - competence damage-inducible protein A (NCBI), at 2,821,871 to 2,822,368
ygaD
b2701: mltB - membrane-bound lytic murein transglycosylase B (NCBI), at 2,822,513 to 2,823,598
mltB
b2702: srlA - glucitol/sorbitol-specific enzyme IIC component of PTS (NCBI), at 2,823,854 to 2,824,417
srlA
b2703: srlE - glucitol/sorbitol-specific enzyme IIB component of PTS (NCBI), at 2,824,414 to 2,825,373
srlE
Position (kb)
2822
2823
2824 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2821.522 kb on + strand, within recA at 2821.638 kb on + strand, within recA at 2821.701 kb on - strand at 2821.810 kb on - strand at 2821.810 kb on - strand at 2822.013 kb on + strand, within ygaD at 2822.231 kb on - strand, within ygaD at 2822.232 kb on + strand, within ygaD at 2822.333 kb on - strand at 2822.358 kb on + strand at 2822.437 kb on - strand at 2822.488 kb on + strand at 2822.520 kb on + strand at 2822.520 kb on + strand at 2822.545 kb on - strand at 2822.598 kb on - strand at 2822.641 kb on - strand, within mltB at 2822.668 kb on + strand, within mltB at 2822.764 kb on - strand, within mltB at 2822.765 kb on - strand, within mltB at 2822.827 kb on + strand, within mltB at 2822.840 kb on - strand, within mltB at 2822.880 kb on - strand, within mltB at 2822.996 kb on + strand, within mltB at 2823.026 kb on - strand, within mltB at 2823.150 kb on + strand, within mltB at 2823.162 kb on + strand, within mltB at 2823.183 kb on + strand, within mltB at 2823.256 kb on + strand, within mltB at 2823.279 kb on - strand, within mltB at 2823.486 kb on - strand, within mltB at 2823.486 kb on - strand, within mltB at 2823.519 kb on - strand at 2823.519 kb on - strand at 2823.633 kb on + strand at 2823.673 kb on - strand at 2823.673 kb on - strand at 2823.689 kb on - strand at 2823.807 kb on - strand at 2823.849 kb on + strand at 2823.872 kb on - strand at 2823.876 kb on + strand at 2823.939 kb on + strand, within srlA at 2823.939 kb on + strand, within srlA at 2823.970 kb on - strand, within srlA at 2823.976 kb on - strand, within srlA at 2824.104 kb on + strand, within srlA at 2824.104 kb on + strand, within srlA at 2824.117 kb on - strand, within srlA at 2824.196 kb on + strand, within srlA at 2824.229 kb on - strand, within srlA at 2824.229 kb on - strand, within srlA at 2824.287 kb on + strand, within srlA at 2824.287 kb on + strand, within srlA at 2824.300 kb on - strand, within srlA at 2824.412 kb on - strand at 2824.412 kb on - strand at 2824.460 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 2,821,522 + recA b2699 0.75 +1.1 2,821,638 + recA b2699 0.85 +2.7 2,821,701 - +0.1 2,821,810 - -0.4 2,821,810 - -0.4 2,822,013 + ygaD b2700 0.29 -0.5 2,822,231 - ygaD b2700 0.72 +0.8 2,822,232 + ygaD b2700 0.72 +0.5 2,822,333 - -3.5 2,822,358 + -1.4 2,822,437 - -1.2 2,822,488 + -0.3 2,822,520 + -0.7 2,822,520 + -2.3 2,822,545 - -0.7 2,822,598 - -2.5 2,822,641 - mltB b2701 0.12 -2.9 2,822,668 + mltB b2701 0.14 -3.5 2,822,764 - mltB b2701 0.23 -3.0 2,822,765 - mltB b2701 0.23 -2.3 2,822,827 + mltB b2701 0.29 -2.1 2,822,840 - mltB b2701 0.30 -3.6 2,822,880 - mltB b2701 0.34 -1.5 2,822,996 + mltB b2701 0.44 -1.7 2,823,026 - mltB b2701 0.47 -1.1 2,823,150 + mltB b2701 0.59 +0.1 2,823,162 + mltB b2701 0.60 -2.7 2,823,183 + mltB b2701 0.62 -2.6 2,823,256 + mltB b2701 0.68 -2.3 2,823,279 - mltB b2701 0.71 -2.1 2,823,486 - mltB b2701 0.90 -2.1 2,823,486 - mltB b2701 0.90 -1.9 2,823,519 - -2.3 2,823,519 - -1.5 2,823,633 + -0.2 2,823,673 - +0.9 2,823,673 - +2.4 2,823,689 - +0.8 2,823,807 - +1.2 2,823,849 + +0.3 2,823,872 - +1.3 2,823,876 + +0.4 2,823,939 + srlA b2702 0.15 -2.7 2,823,939 + srlA b2702 0.15 -1.8 2,823,970 - srlA b2702 0.21 +0.7 2,823,976 - srlA b2702 0.22 +0.3 2,824,104 + srlA b2702 0.44 -0.8 2,824,104 + srlA b2702 0.44 -1.2 2,824,117 - srlA b2702 0.47 -0.9 2,824,196 + srlA b2702 0.61 +0.3 2,824,229 - srlA b2702 0.66 -0.6 2,824,229 - srlA b2702 0.66 -0.3 2,824,287 + srlA b2702 0.77 +0.4 2,824,287 + srlA b2702 0.77 -1.5 2,824,300 - srlA b2702 0.79 +0.1 2,824,412 - -0.5 2,824,412 - -1.7 2,824,460 + -1.3
Or see this region's nucleotide sequence