Strain Fitness in Escherichia coli BW25113 around b2537

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthcaT and hcaR are separated by 159 nucleotideshcaR and hcaE are separated by 135 nucleotides b2536: hcaT - predicted 3-phenylpropionic transporter (NCBI), at 2,664,729 to 2,665,868 hcaT b2537: hcaR - DNA-binding transcriptional activator of 3-phenylpropionic acid catabolism (NCBI), at 2,666,028 to 2,666,918 hcaR b2538: hcaE - 3-phenylpropionate dioxygenase, large (alpha) subunit (NCBI), at 2,667,054 to 2,668,415 hcaE Position (kb) 2666 2667Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2665.029 kb on + strand, within hcaTat 2665.232 kb on + strand, within hcaTat 2665.232 kb on + strand, within hcaTat 2665.242 kb on + strand, within hcaTat 2665.242 kb on + strand, within hcaTat 2665.318 kb on - strand, within hcaTat 2665.358 kb on + strand, within hcaTat 2665.358 kb on + strand, within hcaTat 2665.375 kb on + strand, within hcaTat 2665.375 kb on + strand, within hcaTat 2665.383 kb on - strand, within hcaTat 2665.518 kb on - strand, within hcaTat 2665.518 kb on - strand, within hcaTat 2665.530 kb on - strand, within hcaTat 2665.530 kb on - strand, within hcaTat 2665.556 kb on + strand, within hcaTat 2665.556 kb on + strand, within hcaTat 2665.794 kb on - strandat 2665.794 kb on - strandat 2665.833 kb on + strandat 2665.833 kb on + strandat 2665.896 kb on - strandat 2665.896 kb on - strandat 2665.896 kb on - strandat 2665.896 kb on - strandat 2665.960 kb on + strandat 2666.111 kb on - strandat 2666.203 kb on - strand, within hcaRat 2666.213 kb on + strand, within hcaRat 2666.213 kb on + strand, within hcaRat 2666.216 kb on - strand, within hcaRat 2666.269 kb on - strand, within hcaRat 2666.334 kb on - strand, within hcaRat 2666.359 kb on + strand, within hcaRat 2666.361 kb on + strand, within hcaRat 2666.389 kb on - strand, within hcaRat 2666.389 kb on - strand, within hcaRat 2666.551 kb on + strand, within hcaRat 2666.563 kb on + strand, within hcaRat 2666.824 kb on + strand, within hcaRat 2666.839 kb on - strandat 2666.877 kb on + strandat 2666.877 kb on + strandat 2666.931 kb on - strandat 2667.028 kb on - strandat 2667.028 kb on - strandat 2667.079 kb on + strandat 2667.079 kb on + strandat 2667.364 kb on - strand, within hcaEat 2667.364 kb on - strand, within hcaEat 2667.536 kb on + strand, within hcaEat 2667.646 kb on - strand, within hcaEat 2667.730 kb on - strand, within hcaEat 2667.827 kb on - strand, within hcaEat 2667.880 kb on - strand, within hcaE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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2,665,029 + hcaT b2536 0.26 -1.6
2,665,232 + hcaT b2536 0.44 +1.0
2,665,232 + hcaT b2536 0.44 +2.2
2,665,242 + hcaT b2536 0.45 -0.6
2,665,242 + hcaT b2536 0.45 -1.0
2,665,318 - hcaT b2536 0.52 +0.6
2,665,358 + hcaT b2536 0.55 -0.3
2,665,358 + hcaT b2536 0.55 -0.3
2,665,375 + hcaT b2536 0.57 +0.1
2,665,375 + hcaT b2536 0.57 -0.9
2,665,383 - hcaT b2536 0.57 +0.3
2,665,518 - hcaT b2536 0.69 -0.3
2,665,518 - hcaT b2536 0.69 +0.3
2,665,530 - hcaT b2536 0.70 -0.8
2,665,530 - hcaT b2536 0.70 +0.4
2,665,556 + hcaT b2536 0.73 -1.5
2,665,556 + hcaT b2536 0.73 -0.7
2,665,794 - -0.0
2,665,794 - -1.8
2,665,833 + +0.1
2,665,833 + -1.6
2,665,896 - +0.6
2,665,896 - +0.6
2,665,896 - +0.7
2,665,896 - -2.9
2,665,960 + -1.3
2,666,111 - +1.2
2,666,203 - hcaR b2537 0.20 -1.2
2,666,213 + hcaR b2537 0.21 -0.8
2,666,213 + hcaR b2537 0.21 -0.4
2,666,216 - hcaR b2537 0.21 -1.1
2,666,269 - hcaR b2537 0.27 +0.4
2,666,334 - hcaR b2537 0.34 -0.7
2,666,359 + hcaR b2537 0.37 -0.8
2,666,361 + hcaR b2537 0.37 -0.2
2,666,389 - hcaR b2537 0.41 -1.0
2,666,389 - hcaR b2537 0.41 -0.1
2,666,551 + hcaR b2537 0.59 -0.7
2,666,563 + hcaR b2537 0.60 -2.3
2,666,824 + hcaR b2537 0.89 -1.1
2,666,839 - -0.8
2,666,877 + +0.3
2,666,877 + +0.8
2,666,931 - -0.5
2,667,028 - -0.3
2,667,028 - -0.7
2,667,079 + -0.2
2,667,079 + +0.5
2,667,364 - hcaE b2538 0.23 +0.0
2,667,364 - hcaE b2538 0.23 -1.4
2,667,536 + hcaE b2538 0.35 +0.5
2,667,646 - hcaE b2538 0.43 -0.3
2,667,730 - hcaE b2538 0.50 +0.9
2,667,827 - hcaE b2538 0.57 -1.1
2,667,880 - hcaE b2538 0.61 -0.1

Or see this region's nucleotide sequence