Strain Fitness in Escherichia coli BW25113 around b2518

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfgB and ndk are separated by 149 nucleotidesndk and pbpC are separated by 148 nucleotides b2517: yfgB - predicted enzyme (NCBI), at 2,641,151 to 2,642,305 yfgB b2518: ndk - nucleoside diphosphate kinase (NCBI), at 2,642,455 to 2,642,886 ndk b2519: pbpC - fused transglycosylase/transpeptidase (NCBI), at 2,643,035 to 2,645,347 pbpC Position (kb) 2642 2643Strain fitness (log2 ratio) -2 -1 0 1 2at 2641.671 kb on + strand, within yfgBat 2641.688 kb on + strand, within yfgBat 2641.748 kb on + strand, within yfgBat 2641.748 kb on + strand, within yfgBat 2641.784 kb on - strand, within yfgBat 2641.819 kb on + strand, within yfgBat 2641.819 kb on + strand, within yfgBat 2641.923 kb on - strand, within yfgBat 2641.947 kb on + strand, within yfgBat 2641.947 kb on + strand, within yfgBat 2642.153 kb on - strand, within yfgBat 2642.153 kb on - strand, within yfgBat 2642.304 kb on - strandat 2642.392 kb on - strandat 2642.392 kb on - strandat 2642.509 kb on - strand, within ndkat 2642.509 kb on - strand, within ndkat 2642.590 kb on - strand, within ndkat 2642.592 kb on + strand, within ndkat 2642.684 kb on - strandat 2642.740 kb on - strand, within ndkat 2642.740 kb on - strand, within ndkat 2642.809 kb on + strand, within ndkat 2642.937 kb on + strandat 2643.036 kb on - strandat 2643.081 kb on + strandat 2643.131 kb on - strandat 2643.171 kb on - strandat 2643.235 kb on - strandat 2643.235 kb on - strandat 2643.567 kb on + strand, within pbpCat 2643.567 kb on + strand, within pbpCat 2643.567 kb on + strand, within pbpCat 2643.575 kb on - strand, within pbpCat 2643.627 kb on + strand, within pbpCat 2643.645 kb on - strand, within pbpCat 2643.690 kb on + strand, within pbpC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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2,641,671 + yfgB b2517 0.45 -0.6
2,641,688 + yfgB b2517 0.46 -1.8
2,641,748 + yfgB b2517 0.52 -1.6
2,641,748 + yfgB b2517 0.52 -1.8
2,641,784 - yfgB b2517 0.55 -2.5
2,641,819 + yfgB b2517 0.58 -1.2
2,641,819 + yfgB b2517 0.58 -2.2
2,641,923 - yfgB b2517 0.67 -2.4
2,641,947 + yfgB b2517 0.69 -0.8
2,641,947 + yfgB b2517 0.69 -2.5
2,642,153 - yfgB b2517 0.87 -1.9
2,642,153 - yfgB b2517 0.87 -1.9
2,642,304 - -1.2
2,642,392 - -0.7
2,642,392 - +1.0
2,642,509 - ndk b2518 0.12 +0.0
2,642,509 - ndk b2518 0.12 +0.8
2,642,590 - ndk b2518 0.31 +0.4
2,642,592 + ndk b2518 0.32 +1.0
2,642,684 - +0.0
2,642,740 - ndk b2518 0.66 -1.5
2,642,740 - ndk b2518 0.66 +1.0
2,642,809 + ndk b2518 0.82 +0.0
2,642,937 + -1.3
2,643,036 - +1.3
2,643,081 + -0.5
2,643,131 - +0.4
2,643,171 - -2.1
2,643,235 - -0.6
2,643,235 - -0.8
2,643,567 + pbpC b2519 0.23 -0.5
2,643,567 + pbpC b2519 0.23 -0.2
2,643,567 + pbpC b2519 0.23 +0.6
2,643,575 - pbpC b2519 0.23 +0.3
2,643,627 + pbpC b2519 0.26 +2.6
2,643,645 - pbpC b2519 0.26 -1.3
2,643,690 + pbpC b2519 0.28 -0.5

Or see this region's nucleotide sequence