Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfgA and yfgB are separated by 284 nucleotides yfgB and ndk are separated by 149 nucleotides ndk and pbpC are separated by 148 nucleotides
b2516: yfgA - hypothetical protein (NCBI), at 2,639,853 to 2,640,866
yfgA
b2517: yfgB - predicted enzyme (NCBI), at 2,641,151 to 2,642,305
yfgB
b2518: ndk - nucleoside diphosphate kinase (NCBI), at 2,642,455 to 2,642,886
ndk
b2519: pbpC - fused transglycosylase/transpeptidase (NCBI), at 2,643,035 to 2,645,347
pbpC
Position (kb)
2641
2642
2643 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2640.158 kb on + strand, within yfgA at 2640.262 kb on - strand, within yfgA at 2640.262 kb on - strand, within yfgA at 2640.310 kb on + strand, within yfgA at 2640.315 kb on - strand, within yfgA at 2640.336 kb on + strand, within yfgA at 2640.336 kb on + strand, within yfgA at 2640.347 kb on + strand, within yfgA at 2640.347 kb on + strand, within yfgA at 2640.347 kb on + strand, within yfgA at 2640.348 kb on + strand, within yfgA at 2640.384 kb on + strand, within yfgA at 2640.387 kb on - strand, within yfgA at 2640.442 kb on + strand, within yfgA at 2640.442 kb on + strand, within yfgA at 2640.953 kb on + strand at 2640.955 kb on + strand at 2640.963 kb on + strand at 2640.963 kb on + strand at 2640.963 kb on + strand at 2640.963 kb on + strand at 2640.963 kb on + strand at 2640.963 kb on + strand at 2640.971 kb on - strand at 2640.971 kb on - strand at 2641.026 kb on - strand at 2641.028 kb on + strand at 2641.038 kb on + strand at 2641.046 kb on - strand at 2641.046 kb on - strand at 2641.046 kb on - strand at 2641.173 kb on + strand at 2641.179 kb on + strand at 2641.181 kb on - strand at 2641.181 kb on - strand at 2641.187 kb on - strand at 2641.279 kb on + strand, within yfgB at 2641.279 kb on + strand, within yfgB at 2641.299 kb on - strand, within yfgB at 2641.344 kb on + strand, within yfgB at 2641.397 kb on - strand, within yfgB at 2641.453 kb on - strand, within yfgB at 2641.453 kb on - strand, within yfgB at 2641.671 kb on + strand, within yfgB at 2641.688 kb on + strand, within yfgB at 2641.748 kb on + strand, within yfgB at 2641.748 kb on + strand, within yfgB at 2641.784 kb on - strand, within yfgB at 2641.819 kb on + strand, within yfgB at 2641.819 kb on + strand, within yfgB at 2641.923 kb on - strand, within yfgB at 2641.947 kb on + strand, within yfgB at 2641.947 kb on + strand, within yfgB at 2642.153 kb on - strand, within yfgB at 2642.153 kb on - strand, within yfgB at 2642.304 kb on - strand at 2642.392 kb on - strand at 2642.392 kb on - strand at 2642.509 kb on - strand, within ndk at 2642.509 kb on - strand, within ndk at 2642.590 kb on - strand, within ndk at 2642.592 kb on + strand, within ndk at 2642.684 kb on - strand at 2642.740 kb on - strand, within ndk at 2642.740 kb on - strand, within ndk at 2642.809 kb on + strand, within ndk at 2642.937 kb on + strand at 2643.036 kb on - strand at 2643.081 kb on + strand at 2643.131 kb on - strand at 2643.171 kb on - strand at 2643.235 kb on - strand at 2643.235 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 2,640,158 + yfgA b2516 0.30 -0.1 2,640,262 - yfgA b2516 0.40 +0.5 2,640,262 - yfgA b2516 0.40 +0.4 2,640,310 + yfgA b2516 0.45 -0.8 2,640,315 - yfgA b2516 0.46 +1.8 2,640,336 + yfgA b2516 0.48 -0.3 2,640,336 + yfgA b2516 0.48 -1.0 2,640,347 + yfgA b2516 0.49 +0.0 2,640,347 + yfgA b2516 0.49 -1.3 2,640,347 + yfgA b2516 0.49 -0.6 2,640,348 + yfgA b2516 0.49 +0.0 2,640,384 + yfgA b2516 0.52 -0.6 2,640,387 - yfgA b2516 0.53 -1.2 2,640,442 + yfgA b2516 0.58 -2.0 2,640,442 + yfgA b2516 0.58 -1.2 2,640,953 + +1.0 2,640,955 + +0.5 2,640,963 + -1.4 2,640,963 + -0.3 2,640,963 + +0.2 2,640,963 + -1.3 2,640,963 + -1.0 2,640,963 + -0.2 2,640,971 - -1.3 2,640,971 - -1.2 2,641,026 - -0.6 2,641,028 + -1.6 2,641,038 + -2.3 2,641,046 - +0.5 2,641,046 - -0.5 2,641,046 - -3.2 2,641,173 + -0.6 2,641,179 + +0.0 2,641,181 - -1.0 2,641,181 - -0.8 2,641,187 - -0.1 2,641,279 + yfgB b2517 0.11 -0.4 2,641,279 + yfgB b2517 0.11 -1.5 2,641,299 - yfgB b2517 0.13 -2.1 2,641,344 + yfgB b2517 0.17 -1.8 2,641,397 - yfgB b2517 0.21 -1.9 2,641,453 - yfgB b2517 0.26 -0.3 2,641,453 - yfgB b2517 0.26 -1.4 2,641,671 + yfgB b2517 0.45 -0.6 2,641,688 + yfgB b2517 0.46 -1.8 2,641,748 + yfgB b2517 0.52 -1.6 2,641,748 + yfgB b2517 0.52 -1.8 2,641,784 - yfgB b2517 0.55 -2.5 2,641,819 + yfgB b2517 0.58 -1.2 2,641,819 + yfgB b2517 0.58 -2.2 2,641,923 - yfgB b2517 0.67 -2.4 2,641,947 + yfgB b2517 0.69 -0.8 2,641,947 + yfgB b2517 0.69 -2.5 2,642,153 - yfgB b2517 0.87 -1.9 2,642,153 - yfgB b2517 0.87 -1.9 2,642,304 - -1.2 2,642,392 - -0.7 2,642,392 - +1.0 2,642,509 - ndk b2518 0.12 +0.0 2,642,509 - ndk b2518 0.12 +0.8 2,642,590 - ndk b2518 0.31 +0.4 2,642,592 + ndk b2518 0.32 +1.0 2,642,684 - +0.0 2,642,740 - ndk b2518 0.66 -1.5 2,642,740 - ndk b2518 0.66 +1.0 2,642,809 + ndk b2518 0.82 +0.0 2,642,937 + -1.3 2,643,036 - +1.3 2,643,081 + -0.5 2,643,131 - +0.4 2,643,171 - -2.1 2,643,235 - -0.6 2,643,235 - -0.8
Or see this region's nucleotide sequence