Strain Fitness in Escherichia coli BW25113 around b2341

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntsixA and yfcX are separated by 202 nucleotidesyfcX and yfcY overlap by 1 nucleotides b2340: sixA - phosphohistidine phosphatase (NCBI), at 2,454,349 to 2,454,834 sixA b2341: yfcX - fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase (NCBI), at 2,455,037 to 2,457,181 yfcX b2342: yfcY - acetyl-CoA acetyltransferase (NCBI), at 2,457,181 to 2,458,491 yfcY Position (kb) 2455 2456 2457 2458Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2454.088 kb on - strandat 2454.088 kb on - strandat 2454.178 kb on - strandat 2454.185 kb on - strandat 2454.201 kb on - strandat 2454.201 kb on - strandat 2454.216 kb on - strandat 2454.268 kb on - strandat 2454.275 kb on - strandat 2454.275 kb on - strandat 2454.280 kb on + strandat 2454.290 kb on - strandat 2454.290 kb on - strandat 2454.295 kb on - strandat 2454.295 kb on - strandat 2454.330 kb on - strandat 2454.375 kb on - strandat 2454.375 kb on - strandat 2454.389 kb on - strandat 2454.476 kb on + strand, within sixAat 2454.476 kb on + strand, within sixAat 2454.482 kb on - strand, within sixAat 2454.509 kb on + strand, within sixAat 2454.520 kb on + strand, within sixAat 2454.535 kb on - strand, within sixAat 2454.535 kb on - strand, within sixAat 2454.550 kb on - strand, within sixAat 2454.584 kb on - strand, within sixAat 2454.584 kb on - strand, within sixAat 2455.035 kb on + strandat 2455.071 kb on + strandat 2455.071 kb on + strandat 2455.084 kb on + strandat 2455.185 kb on + strandat 2455.185 kb on + strandat 2455.262 kb on + strand, within yfcXat 2455.268 kb on + strand, within yfcXat 2455.321 kb on + strand, within yfcXat 2455.414 kb on - strand, within yfcXat 2455.418 kb on - strand, within yfcXat 2455.587 kb on - strand, within yfcXat 2455.620 kb on + strand, within yfcXat 2455.796 kb on - strand, within yfcXat 2456.145 kb on + strand, within yfcXat 2456.145 kb on + strand, within yfcXat 2456.175 kb on - strand, within yfcXat 2456.253 kb on + strand, within yfcXat 2456.253 kb on + strand, within yfcXat 2456.383 kb on + strand, within yfcXat 2456.402 kb on - strand, within yfcXat 2456.425 kb on + strand, within yfcXat 2456.425 kb on + strand, within yfcXat 2456.544 kb on + strand, within yfcXat 2456.614 kb on + strand, within yfcXat 2456.614 kb on + strand, within yfcXat 2456.617 kb on - strand, within yfcXat 2456.617 kb on - strand, within yfcXat 2456.663 kb on - strand, within yfcXat 2456.684 kb on + strand, within yfcXat 2456.747 kb on + strand, within yfcXat 2456.747 kb on + strand, within yfcXat 2456.771 kb on + strand, within yfcXat 2456.771 kb on + strand, within yfcXat 2457.130 kb on - strandat 2457.130 kb on - strandat 2457.241 kb on + strandat 2457.268 kb on - strandat 2457.326 kb on - strand, within yfcYat 2457.381 kb on + strand, within yfcYat 2457.473 kb on + strand, within yfcYat 2457.532 kb on + strand, within yfcYat 2457.532 kb on + strand, within yfcYat 2457.535 kb on - strand, within yfcYat 2457.539 kb on - strand, within yfcYat 2457.561 kb on + strand, within yfcYat 2457.636 kb on - strand, within yfcYat 2457.876 kb on + strand, within yfcY

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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2,454,088 - +1.0
2,454,088 - -0.0
2,454,178 - -0.2
2,454,185 - +1.4
2,454,201 - +1.0
2,454,201 - +0.7
2,454,216 - -0.6
2,454,268 - -1.0
2,454,275 - +3.0
2,454,275 - +1.0
2,454,280 + -0.6
2,454,290 - -0.0
2,454,290 - -1.3
2,454,295 - +1.0
2,454,295 - -2.2
2,454,330 - +1.2
2,454,375 - +1.0
2,454,375 - +0.7
2,454,389 - -0.6
2,454,476 + sixA b2340 0.26 +1.0
2,454,476 + sixA b2340 0.26 +1.6
2,454,482 - sixA b2340 0.27 +1.3
2,454,509 + sixA b2340 0.33 +1.7
2,454,520 + sixA b2340 0.35 -1.0
2,454,535 - sixA b2340 0.38 +1.0
2,454,535 - sixA b2340 0.38 +2.1
2,454,550 - sixA b2340 0.41 +1.5
2,454,584 - sixA b2340 0.48 +0.9
2,454,584 - sixA b2340 0.48 +1.1
2,455,035 + -1.6
2,455,071 + -2.8
2,455,071 + -0.6
2,455,084 + -0.3
2,455,185 + -1.0
2,455,185 + +1.4
2,455,262 + yfcX b2341 0.10 +0.4
2,455,268 + yfcX b2341 0.11 -0.6
2,455,321 + yfcX b2341 0.13 -0.7
2,455,414 - yfcX b2341 0.18 -1.0
2,455,418 - yfcX b2341 0.18 -2.1
2,455,587 - yfcX b2341 0.26 -0.6
2,455,620 + yfcX b2341 0.27 -1.3
2,455,796 - yfcX b2341 0.35 -1.6
2,456,145 + yfcX b2341 0.52 +0.2
2,456,145 + yfcX b2341 0.52 -0.1
2,456,175 - yfcX b2341 0.53 -0.7
2,456,253 + yfcX b2341 0.57 -0.5
2,456,253 + yfcX b2341 0.57 -1.1
2,456,383 + yfcX b2341 0.63 -0.7
2,456,402 - yfcX b2341 0.64 +0.4
2,456,425 + yfcX b2341 0.65 -1.4
2,456,425 + yfcX b2341 0.65 -1.8
2,456,544 + yfcX b2341 0.70 +1.0
2,456,614 + yfcX b2341 0.74 -0.0
2,456,614 + yfcX b2341 0.74 +0.7
2,456,617 - yfcX b2341 0.74 +0.4
2,456,617 - yfcX b2341 0.74 -1.5
2,456,663 - yfcX b2341 0.76 +0.2
2,456,684 + yfcX b2341 0.77 +0.4
2,456,747 + yfcX b2341 0.80 -0.2
2,456,747 + yfcX b2341 0.80 +1.2
2,456,771 + yfcX b2341 0.81 -0.6
2,456,771 + yfcX b2341 0.81 +2.6
2,457,130 - -1.0
2,457,130 - -1.6
2,457,241 + -1.1
2,457,268 - -1.9
2,457,326 - yfcY b2342 0.11 -1.5
2,457,381 + yfcY b2342 0.15 -0.7
2,457,473 + yfcY b2342 0.22 -0.5
2,457,532 + yfcY b2342 0.27 -1.3
2,457,532 + yfcY b2342 0.27 -1.5
2,457,535 - yfcY b2342 0.27 +0.6
2,457,539 - yfcY b2342 0.27 -1.5
2,457,561 + yfcY b2342 0.29 -1.4
2,457,636 - yfcY b2342 0.35 -0.3
2,457,876 + yfcY b2342 0.53 -1.5

Or see this region's nucleotide sequence