Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliC and fliD are separated by 265 nucleotides fliD and fliS are separated by 24 nucleotides fliS and fliT overlap by 1 nucleotides fliT and amyA are separated by 77 nucleotides
b1923: fliC - flagellin (NCBI), at 2,000,134 to 2,001,630
fliC
b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302
fliD
b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737
fliS
b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102
fliT
b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667
amyA
Position (kb)
2001
2002
2003
2004 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2000.935 kb on + strand, within fliC at 2000.935 kb on + strand, within fliC at 2000.954 kb on + strand, within fliC at 2000.981 kb on - strand, within fliC at 2001.074 kb on - strand, within fliC at 2001.074 kb on - strand, within fliC at 2001.083 kb on - strand, within fliC at 2001.083 kb on - strand, within fliC at 2001.090 kb on - strand, within fliC at 2001.090 kb on - strand, within fliC at 2001.329 kb on + strand, within fliC at 2001.337 kb on - strand, within fliC at 2001.337 kb on - strand, within fliC at 2001.400 kb on + strand, within fliC at 2001.499 kb on - strand at 2001.522 kb on - strand at 2001.522 kb on - strand at 2001.548 kb on + strand at 2001.548 kb on + strand at 2001.706 kb on + strand at 2001.706 kb on + strand at 2001.842 kb on - strand at 2001.923 kb on + strand at 2001.964 kb on - strand at 2002.119 kb on + strand, within fliD at 2002.119 kb on + strand, within fliD at 2002.190 kb on - strand, within fliD at 2002.353 kb on + strand, within fliD at 2002.456 kb on + strand, within fliD at 2002.456 kb on + strand, within fliD at 2002.547 kb on - strand, within fliD at 2002.664 kb on - strand, within fliD at 2002.664 kb on - strand, within fliD at 2002.732 kb on - strand, within fliD at 2002.829 kb on - strand, within fliD at 2002.892 kb on - strand, within fliD at 2002.894 kb on - strand, within fliD at 2002.894 kb on - strand, within fliD at 2002.913 kb on - strand, within fliD at 2003.080 kb on - strand, within fliD at 2003.414 kb on + strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.462 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.699 kb on + strand at 2003.734 kb on + strand at 2003.735 kb on - strand at 2003.738 kb on - strand at 2003.923 kb on - strand, within fliT at 2003.933 kb on - strand, within fliT at 2004.035 kb on + strand, within fliT at 2004.073 kb on + strand at 2004.135 kb on + strand at 2004.148 kb on + strand at 2004.241 kb on + strand at 2004.241 kb on + strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.299 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 2,000,935 + fliC b1923 0.54 -1.8 2,000,935 + fliC b1923 0.54 -0.6 2,000,954 + fliC b1923 0.55 -2.1 2,000,981 - fliC b1923 0.57 -1.4 2,001,074 - fliC b1923 0.63 -0.7 2,001,074 - fliC b1923 0.63 -1.9 2,001,083 - fliC b1923 0.63 +0.3 2,001,083 - fliC b1923 0.63 +0.9 2,001,090 - fliC b1923 0.64 -2.1 2,001,090 - fliC b1923 0.64 -0.9 2,001,329 + fliC b1923 0.80 -1.2 2,001,337 - fliC b1923 0.80 -1.1 2,001,337 - fliC b1923 0.80 -3.0 2,001,400 + fliC b1923 0.85 -1.7 2,001,499 - -1.1 2,001,522 - +0.9 2,001,522 - +0.9 2,001,548 + -1.4 2,001,548 + -1.4 2,001,706 + -0.1 2,001,706 + -0.1 2,001,842 - -1.5 2,001,923 + +0.9 2,001,964 - -3.3 2,002,119 + fliD b1924 0.16 +1.9 2,002,119 + fliD b1924 0.16 +0.9 2,002,190 - fliD b1924 0.21 -1.9 2,002,353 + fliD b1924 0.32 -2.3 2,002,456 + fliD b1924 0.40 -1.7 2,002,456 + fliD b1924 0.40 -1.9 2,002,547 - fliD b1924 0.46 -0.8 2,002,664 - fliD b1924 0.55 -1.4 2,002,664 - fliD b1924 0.55 -2.7 2,002,732 - fliD b1924 0.59 -1.1 2,002,829 - fliD b1924 0.66 -1.0 2,002,892 - fliD b1924 0.71 -2.7 2,002,894 - fliD b1924 0.71 -1.9 2,002,894 - fliD b1924 0.71 -1.1 2,002,913 - fliD b1924 0.72 -2.6 2,003,080 - fliD b1924 0.84 -1.9 2,003,414 + fliS b1925 0.21 -0.2 2,003,451 - fliS b1925 0.30 -2.7 2,003,451 - fliS b1925 0.30 -1.9 2,003,451 - fliS b1925 0.30 -0.7 2,003,462 + fliS b1925 0.33 +0.3 2,003,544 + fliS b1925 0.53 -2.4 2,003,544 + fliS b1925 0.53 +0.6 2,003,699 + -0.3 2,003,734 + +0.1 2,003,735 - -2.3 2,003,738 - -0.3 2,003,923 - fliT b1926 0.51 -2.6 2,003,933 - fliT b1926 0.54 +3.4 2,004,035 + fliT b1926 0.81 +0.5 2,004,073 + +2.2 2,004,135 + +2.0 2,004,148 + +0.1 2,004,241 + -0.7 2,004,241 + +0.9 2,004,249 - +0.0 2,004,249 - -0.1 2,004,249 - -0.1 2,004,299 + +0.8
Or see this region's nucleotide sequence