Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliA and fliC are separated by 320 nucleotides fliC and fliD are separated by 265 nucleotides
b1922: fliA - flagellar biosynthesis; alternative sigma factor 28; regulation of flagellar operons (VIMSS), at 1,999,094 to 1,999,813
fliA
b1923: fliC - flagellin (NCBI), at 2,000,134 to 2,001,630
fliC
b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302
fliD
Position (kb)
2000
2001
2002 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1999.139 kb on + strand at 1999.145 kb on - strand at 1999.145 kb on - strand at 1999.177 kb on - strand, within fliA at 1999.209 kb on - strand, within fliA at 1999.292 kb on - strand, within fliA at 1999.292 kb on - strand, within fliA at 1999.302 kb on - strand, within fliA at 1999.504 kb on - strand, within fliA at 1999.504 kb on - strand, within fliA at 1999.508 kb on - strand, within fliA at 1999.509 kb on - strand, within fliA at 1999.509 kb on - strand, within fliA at 1999.541 kb on + strand, within fliA at 1999.595 kb on - strand, within fliA at 1999.600 kb on - strand, within fliA at 1999.615 kb on + strand, within fliA at 1999.655 kb on + strand, within fliA at 1999.800 kb on - strand at 1999.832 kb on + strand at 1999.903 kb on - strand at 1999.969 kb on + strand at 1999.969 kb on + strand at 2000.236 kb on - strand at 2000.439 kb on - strand, within fliC at 2000.523 kb on + strand, within fliC at 2000.591 kb on + strand, within fliC at 2000.591 kb on + strand, within fliC at 2000.766 kb on - strand, within fliC at 2000.772 kb on + strand, within fliC at 2000.772 kb on + strand, within fliC at 2000.849 kb on - strand, within fliC at 2000.935 kb on + strand, within fliC at 2000.935 kb on + strand, within fliC at 2000.954 kb on + strand, within fliC at 2000.981 kb on - strand, within fliC at 2001.074 kb on - strand, within fliC at 2001.074 kb on - strand, within fliC at 2001.083 kb on - strand, within fliC at 2001.083 kb on - strand, within fliC at 2001.090 kb on - strand, within fliC at 2001.090 kb on - strand, within fliC at 2001.329 kb on + strand, within fliC at 2001.337 kb on - strand, within fliC at 2001.337 kb on - strand, within fliC at 2001.400 kb on + strand, within fliC at 2001.499 kb on - strand at 2001.522 kb on - strand at 2001.522 kb on - strand at 2001.548 kb on + strand at 2001.548 kb on + strand at 2001.706 kb on + strand at 2001.706 kb on + strand at 2001.842 kb on - strand at 2001.923 kb on + strand at 2001.964 kb on - strand at 2002.119 kb on + strand, within fliD at 2002.119 kb on + strand, within fliD at 2002.190 kb on - strand, within fliD at 2002.353 kb on + strand, within fliD at 2002.456 kb on + strand, within fliD at 2002.456 kb on + strand, within fliD at 2002.547 kb on - strand, within fliD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 1,999,139 + -1.4 1,999,145 - -1.4 1,999,145 - +2.1 1,999,177 - fliA b1922 0.12 -0.0 1,999,209 - fliA b1922 0.16 -0.8 1,999,292 - fliA b1922 0.28 -0.1 1,999,292 - fliA b1922 0.28 -0.7 1,999,302 - fliA b1922 0.29 -2.8 1,999,504 - fliA b1922 0.57 -1.4 1,999,504 - fliA b1922 0.57 +0.9 1,999,508 - fliA b1922 0.57 +0.9 1,999,509 - fliA b1922 0.58 +1.2 1,999,509 - fliA b1922 0.58 -0.3 1,999,541 + fliA b1922 0.62 -0.1 1,999,595 - fliA b1922 0.70 -0.3 1,999,600 - fliA b1922 0.70 -0.8 1,999,615 + fliA b1922 0.72 -0.7 1,999,655 + fliA b1922 0.78 -1.8 1,999,800 - -1.8 1,999,832 + +0.1 1,999,903 - -1.7 1,999,969 + -1.8 1,999,969 + -1.9 2,000,236 - -0.9 2,000,439 - fliC b1923 0.20 -1.4 2,000,523 + fliC b1923 0.26 -2.8 2,000,591 + fliC b1923 0.31 -1.9 2,000,591 + fliC b1923 0.31 -0.7 2,000,766 - fliC b1923 0.42 -0.4 2,000,772 + fliC b1923 0.43 -0.1 2,000,772 + fliC b1923 0.43 +0.2 2,000,849 - fliC b1923 0.48 -1.3 2,000,935 + fliC b1923 0.54 -1.8 2,000,935 + fliC b1923 0.54 -0.6 2,000,954 + fliC b1923 0.55 -2.1 2,000,981 - fliC b1923 0.57 -1.4 2,001,074 - fliC b1923 0.63 -0.7 2,001,074 - fliC b1923 0.63 -1.9 2,001,083 - fliC b1923 0.63 +0.3 2,001,083 - fliC b1923 0.63 +0.9 2,001,090 - fliC b1923 0.64 -2.1 2,001,090 - fliC b1923 0.64 -0.9 2,001,329 + fliC b1923 0.80 -1.2 2,001,337 - fliC b1923 0.80 -1.1 2,001,337 - fliC b1923 0.80 -3.0 2,001,400 + fliC b1923 0.85 -1.7 2,001,499 - -1.1 2,001,522 - +0.9 2,001,522 - +0.9 2,001,548 + -1.4 2,001,548 + -1.4 2,001,706 + -0.1 2,001,706 + -0.1 2,001,842 - -1.5 2,001,923 + +0.9 2,001,964 - -3.3 2,002,119 + fliD b1924 0.16 +1.9 2,002,119 + fliD b1924 0.16 +0.9 2,002,190 - fliD b1924 0.21 -1.9 2,002,353 + fliD b1924 0.32 -2.3 2,002,456 + fliD b1924 0.40 -1.7 2,002,456 + fliD b1924 0.40 -1.9 2,002,547 - fliD b1924 0.46 -0.8
Or see this region's nucleotide sequence