Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cheA and motB are separated by 4 nucleotides motB and motA overlap by 4 nucleotides
b1888: cheA - fused chemotactic sensory histidine kinase in two-component regulatory system with CheB and CheY: sensory histidine kinase/signal sensing protein (NCBI), at 1,971,384 to 1,973,348
cheA
b1889: motB - flagellar motor protein MotB (NCBI), at 1,973,353 to 1,974,279
motB
b1890: motA - flagellar motor protein MotA (NCBI), at 1,974,276 to 1,975,163
motA
Position (kb)
1973
1974
1975 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1972.498 kb on + strand, within cheA at 1972.631 kb on + strand, within cheA at 1972.777 kb on + strand, within cheA at 1972.859 kb on + strand, within cheA at 1972.874 kb on + strand, within cheA at 1972.963 kb on + strand, within cheA at 1973.015 kb on - strand, within cheA at 1973.103 kb on - strand, within cheA at 1973.279 kb on - strand at 1973.279 kb on - strand at 1973.443 kb on - strand at 1973.443 kb on - strand at 1973.577 kb on - strand, within motB at 1973.595 kb on - strand, within motB at 1973.697 kb on + strand, within motB at 1973.846 kb on - strand, within motB at 1973.850 kb on - strand, within motB at 1974.089 kb on - strand, within motB at 1974.089 kb on - strand, within motB at 1974.197 kb on + strand at 1974.226 kb on + strand at 1974.233 kb on + strand at 1974.233 kb on + strand at 1974.414 kb on + strand, within motA at 1974.854 kb on - strand, within motA at 1974.934 kb on + strand, within motA at 1974.934 kb on + strand, within motA at 1975.040 kb on + strand, within motA at 1975.092 kb on + strand at 1975.093 kb on - strand at 1975.173 kb on + strand at 1975.179 kb on + strand at 1975.179 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 1,972,498 + cheA b1888 0.57 +0.2 1,972,631 + cheA b1888 0.63 +0.4 1,972,777 + cheA b1888 0.71 +0.4 1,972,859 + cheA b1888 0.75 +0.5 1,972,874 + cheA b1888 0.76 -1.4 1,972,963 + cheA b1888 0.80 -1.3 1,973,015 - cheA b1888 0.83 -0.9 1,973,103 - cheA b1888 0.87 -2.9 1,973,279 - -1.8 1,973,279 - -0.6 1,973,443 - +3.2 1,973,443 - -0.4 1,973,577 - motB b1889 0.24 -1.5 1,973,595 - motB b1889 0.26 -3.9 1,973,697 + motB b1889 0.37 -1.2 1,973,846 - motB b1889 0.53 -1.2 1,973,850 - motB b1889 0.54 -2.0 1,974,089 - motB b1889 0.79 -1.7 1,974,089 - motB b1889 0.79 -2.4 1,974,197 + -1.1 1,974,226 + -2.6 1,974,233 + -2.7 1,974,233 + +0.7 1,974,414 + motA b1890 0.16 -2.9 1,974,854 - motA b1890 0.65 -1.1 1,974,934 + motA b1890 0.74 -2.2 1,974,934 + motA b1890 0.74 -0.4 1,975,040 + motA b1890 0.86 -0.7 1,975,092 + -1.7 1,975,093 - -0.2 1,975,173 + -0.9 1,975,179 + -1.8 1,975,179 + -1.4
Or see this region's nucleotide sequence