Strain Fitness in Escherichia coli BW25113 around b1814

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpabB and yeaB are separated by 3 nucleotidesyeaB and sdaA are separated by 183 nucleotidessdaA and b1815 are separated by 130 nucleotides b1812: pabB - para-aminobenzoate synthase component I (NCBI), at 1,892,829 to 1,894,190 pabB b1813: yeaB - predicted NUDIX hydrolase (NCBI), at 1,894,194 to 1,894,772 yeaB b1814: sdaA - L-serine deaminase I (NCBI), at 1,894,956 to 1,896,320 sdaA b1815: b1815 - orf, hypothetical protein (VIMSS), at 1,896,451 to 1,898,049 b1815 Position (kb) 1894 1895 1896 1897Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1893.960 kb on - strand, within pabBat 1893.960 kb on - strand, within pabBat 1894.035 kb on + strand, within pabBat 1894.301 kb on - strand, within yeaBat 1894.400 kb on + strand, within yeaBat 1894.406 kb on - strand, within yeaBat 1894.419 kb on + strand, within yeaBat 1894.446 kb on + strand, within yeaBat 1894.456 kb on + strand, within yeaBat 1894.500 kb on + strand, within yeaBat 1894.500 kb on + strand, within yeaBat 1894.551 kb on - strand, within yeaBat 1894.579 kb on - strand, within yeaBat 1894.629 kb on - strand, within yeaBat 1894.683 kb on + strand, within yeaBat 1894.683 kb on + strand, within yeaBat 1894.686 kb on - strand, within yeaBat 1894.693 kb on + strand, within yeaBat 1894.693 kb on + strand, within yeaBat 1894.735 kb on + strandat 1894.766 kb on + strandat 1894.766 kb on + strandat 1894.804 kb on + strandat 1894.875 kb on - strandat 1894.875 kb on - strandat 1894.890 kb on + strandat 1894.890 kb on + strandat 1894.915 kb on - strandat 1894.961 kb on - strandat 1894.997 kb on + strandat 1895.002 kb on - strandat 1895.028 kb on - strandat 1895.028 kb on - strandat 1895.039 kb on - strandat 1895.039 kb on - strandat 1895.465 kb on + strand, within sdaAat 1895.498 kb on - strand, within sdaAat 1895.540 kb on - strand, within sdaAat 1895.646 kb on - strand, within sdaAat 1895.646 kb on - strand, within sdaAat 1896.004 kb on - strand, within sdaAat 1896.004 kb on - strand, within sdaAat 1896.254 kb on + strandat 1896.386 kb on - strandat 1896.440 kb on - strandat 1896.440 kb on - strandat 1896.564 kb on + strandat 1896.618 kb on - strand, within b1815at 1896.650 kb on + strand, within b1815at 1896.681 kb on + strand, within b1815at 1896.681 kb on + strand, within b1815at 1896.686 kb on + strand, within b1815at 1896.770 kb on - strand, within b1815at 1896.770 kb on - strand, within b1815at 1896.929 kb on + strand, within b1815at 1896.956 kb on - strand, within b1815at 1897.151 kb on - strand, within b1815

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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1,893,960 - pabB b1812 0.83 -2.1
1,893,960 - pabB b1812 0.83 +0.5
1,894,035 + pabB b1812 0.89 +0.4
1,894,301 - yeaB b1813 0.18 -0.1
1,894,400 + yeaB b1813 0.36 -2.2
1,894,406 - yeaB b1813 0.37 -0.8
1,894,419 + yeaB b1813 0.39 +0.3
1,894,446 + yeaB b1813 0.44 +1.9
1,894,456 + yeaB b1813 0.45 +1.4
1,894,500 + yeaB b1813 0.53 -2.2
1,894,500 + yeaB b1813 0.53 +2.2
1,894,551 - yeaB b1813 0.62 +0.4
1,894,579 - yeaB b1813 0.66 +0.8
1,894,629 - yeaB b1813 0.75 -0.4
1,894,683 + yeaB b1813 0.84 -1.0
1,894,683 + yeaB b1813 0.84 -1.7
1,894,686 - yeaB b1813 0.85 +1.3
1,894,693 + yeaB b1813 0.86 +0.2
1,894,693 + yeaB b1813 0.86 -2.4
1,894,735 + -0.9
1,894,766 + -0.8
1,894,766 + -1.1
1,894,804 + +0.3
1,894,875 - +0.9
1,894,875 - +0.9
1,894,890 + +0.1
1,894,890 + +0.0
1,894,915 - -1.7
1,894,961 - +0.3
1,894,997 + +0.4
1,895,002 - +1.3
1,895,028 - -0.4
1,895,028 - +3.5
1,895,039 - -2.8
1,895,039 - -2.7
1,895,465 + sdaA b1814 0.37 -1.7
1,895,498 - sdaA b1814 0.40 -1.1
1,895,540 - sdaA b1814 0.43 -1.1
1,895,646 - sdaA b1814 0.51 -1.1
1,895,646 - sdaA b1814 0.51 -0.1
1,896,004 - sdaA b1814 0.77 +0.9
1,896,004 - sdaA b1814 0.77 +3.5
1,896,254 + +0.9
1,896,386 - +0.8
1,896,440 - -0.7
1,896,440 - -1.4
1,896,564 + +1.5
1,896,618 - b1815 b1815 0.10 -0.2
1,896,650 + b1815 b1815 0.12 +0.4
1,896,681 + b1815 b1815 0.14 +0.1
1,896,681 + b1815 b1815 0.14 -2.9
1,896,686 + b1815 b1815 0.15 +0.2
1,896,770 - b1815 b1815 0.20 +0.6
1,896,770 - b1815 b1815 0.20 -0.3
1,896,929 + b1815 b1815 0.30 +0.9
1,896,956 - b1815 b1815 0.32 +0.5
1,897,151 - b1815 b1815 0.44 -0.7

Or see this region's nucleotide sequence