Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjE and ydjF are separated by 116 nucleotides ydjF and ydjG are separated by 136 nucleotides
b1769: ydjE - predicted transporter (NCBI), at 1,850,645 to 1,852,003
ydjE
b1770: ydjF - predicted DNA-binding transcriptional regulator (NCBI), at 1,852,120 to 1,852,878
ydjF
b1771: ydjG - predicted oxidoreductase (NCBI), at 1,853,015 to 1,853,995
ydjG
Position (kb)
1852
1853 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1851.163 kb on - strand, within ydjE at 1851.176 kb on - strand, within ydjE at 1851.276 kb on - strand, within ydjE at 1851.280 kb on - strand, within ydjE at 1851.297 kb on - strand, within ydjE at 1851.312 kb on - strand, within ydjE at 1851.312 kb on - strand, within ydjE at 1851.348 kb on - strand, within ydjE at 1851.348 kb on - strand, within ydjE at 1851.364 kb on - strand, within ydjE at 1851.435 kb on - strand, within ydjE at 1851.457 kb on - strand, within ydjE at 1851.521 kb on - strand, within ydjE at 1851.521 kb on - strand, within ydjE at 1851.526 kb on - strand, within ydjE at 1851.666 kb on + strand, within ydjE at 1851.668 kb on + strand, within ydjE at 1851.671 kb on + strand, within ydjE at 1851.671 kb on + strand, within ydjE at 1851.679 kb on - strand, within ydjE at 1851.703 kb on + strand, within ydjE at 1851.716 kb on + strand, within ydjE at 1851.736 kb on + strand, within ydjE at 1851.769 kb on + strand, within ydjE at 1851.774 kb on + strand, within ydjE at 1851.832 kb on + strand, within ydjE at 1851.877 kb on + strand at 1851.968 kb on + strand at 1851.968 kb on + strand at 1851.976 kb on - strand at 1852.035 kb on + strand at 1852.163 kb on - strand at 1852.165 kb on - strand at 1852.165 kb on - strand at 1852.165 kb on - strand at 1852.167 kb on - strand at 1852.172 kb on - strand at 1852.224 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.252 kb on - strand, within ydjF at 1852.264 kb on - strand, within ydjF at 1852.264 kb on - strand, within ydjF at 1852.388 kb on - strand, within ydjF at 1852.457 kb on - strand, within ydjF at 1852.527 kb on + strand, within ydjF at 1852.532 kb on + strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.535 kb on - strand, within ydjF at 1852.576 kb on - strand, within ydjF at 1852.581 kb on - strand, within ydjF at 1852.606 kb on + strand, within ydjF at 1852.611 kb on - strand, within ydjF at 1852.636 kb on - strand, within ydjF at 1852.853 kb on + strand at 1853.029 kb on - strand at 1853.029 kb on - strand at 1853.048 kb on - strand at 1853.102 kb on + strand at 1853.110 kb on - strand at 1853.170 kb on + strand, within ydjG at 1853.192 kb on - strand, within ydjG at 1853.368 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.425 kb on - strand, within ydjG at 1853.448 kb on + strand, within ydjG at 1853.537 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.621 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.666 kb on - strand, within ydjG at 1853.751 kb on + strand, within ydjG at 1853.763 kb on - strand, within ydjG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 1,851,163 - ydjE b1769 0.38 +0.8 1,851,176 - ydjE b1769 0.39 +0.8 1,851,276 - ydjE b1769 0.46 +0.8 1,851,280 - ydjE b1769 0.47 -1.2 1,851,297 - ydjE b1769 0.48 -0.2 1,851,312 - ydjE b1769 0.49 +0.8 1,851,312 - ydjE b1769 0.49 +0.8 1,851,348 - ydjE b1769 0.52 +0.8 1,851,348 - ydjE b1769 0.52 +0.8 1,851,364 - ydjE b1769 0.53 -1.2 1,851,435 - ydjE b1769 0.58 +0.8 1,851,457 - ydjE b1769 0.60 +0.8 1,851,521 - ydjE b1769 0.64 -0.2 1,851,521 - ydjE b1769 0.64 -0.8 1,851,526 - ydjE b1769 0.65 -1.2 1,851,666 + ydjE b1769 0.75 +1.1 1,851,668 + ydjE b1769 0.75 -0.2 1,851,671 + ydjE b1769 0.75 +0.8 1,851,671 + ydjE b1769 0.75 -0.2 1,851,679 - ydjE b1769 0.76 +0.8 1,851,703 + ydjE b1769 0.78 -0.8 1,851,716 + ydjE b1769 0.79 +2.6 1,851,736 + ydjE b1769 0.80 +0.1 1,851,769 + ydjE b1769 0.83 -1.5 1,851,774 + ydjE b1769 0.83 -0.6 1,851,832 + ydjE b1769 0.87 +0.0 1,851,877 + +2.3 1,851,968 + +2.8 1,851,968 + +2.1 1,851,976 - +0.8 1,852,035 + +1.3 1,852,163 - -3.2 1,852,165 - -0.5 1,852,165 - -0.5 1,852,165 - -1.2 1,852,167 - -2.2 1,852,172 - -2.0 1,852,224 - ydjF b1770 0.14 +0.2 1,852,252 - ydjF b1770 0.17 -1.5 1,852,252 - ydjF b1770 0.17 -2.6 1,852,252 - ydjF b1770 0.17 -1.2 1,852,252 - ydjF b1770 0.17 -2.6 1,852,252 - ydjF b1770 0.17 -2.2 1,852,264 - ydjF b1770 0.19 -2.3 1,852,264 - ydjF b1770 0.19 -0.2 1,852,388 - ydjF b1770 0.35 -1.5 1,852,457 - ydjF b1770 0.44 -0.6 1,852,527 + ydjF b1770 0.54 -0.2 1,852,532 + ydjF b1770 0.54 +0.8 1,852,535 - ydjF b1770 0.55 -1.2 1,852,535 - ydjF b1770 0.55 +0.0 1,852,535 - ydjF b1770 0.55 -1.2 1,852,576 - ydjF b1770 0.60 -1.4 1,852,581 - ydjF b1770 0.61 -1.0 1,852,606 + ydjF b1770 0.64 +0.8 1,852,611 - ydjF b1770 0.65 +0.4 1,852,636 - ydjF b1770 0.68 -1.1 1,852,853 + +0.8 1,853,029 - -0.2 1,853,029 - -0.2 1,853,048 - -1.0 1,853,102 + +1.1 1,853,110 - +1.2 1,853,170 + ydjG b1771 0.16 -2.3 1,853,192 - ydjG b1771 0.18 -1.3 1,853,368 - ydjG b1771 0.36 -0.2 1,853,425 - ydjG b1771 0.42 -1.0 1,853,425 - ydjG b1771 0.42 -0.8 1,853,448 + ydjG b1771 0.44 -0.2 1,853,537 - ydjG b1771 0.53 -0.0 1,853,621 - ydjG b1771 0.62 -3.1 1,853,621 - ydjG b1771 0.62 -1.0 1,853,666 - ydjG b1771 0.66 +0.4 1,853,666 - ydjG b1771 0.66 +0.6 1,853,751 + ydjG b1771 0.75 +0.8 1,853,763 - ydjG b1771 0.76 -0.8
Or see this region's nucleotide sequence