Strain Fitness in Escherichia coli BW25113 around b1638

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttyrS and pdxH are separated by 128 nucleotidespdxH and ydhA are separated by 58 nucleotidesydhA and ydhH are separated by 97 nucleotides b1637: tyrS - tyrosyl-tRNA synthetase (NCBI), at 1,713,972 to 1,715,246 tyrS b1638: pdxH - pyridoxamine 5'-phosphate oxidase (NCBI), at 1,715,375 to 1,716,031 pdxH b1639: ydhA - orf, hypothetical protein (VIMSS), at 1,716,090 to 1,716,419 ydhA b1640: ydhH - anhydro-N-acetylmuramic acid kinase (NCBI), at 1,716,517 to 1,717,626 ydhH Position (kb) 1715 1716 1717Strain fitness (log2 ratio) -2 -1 0 1at 1715.267 kb on - strandat 1715.267 kb on - strandat 1716.201 kb on - strand, within ydhAat 1716.224 kb on + strand, within ydhAat 1716.226 kb on + strand, within ydhAat 1716.226 kb on + strand, within ydhAat 1716.370 kb on - strand, within ydhAat 1716.375 kb on + strand, within ydhAat 1716.375 kb on + strand, within ydhAat 1716.447 kb on + strandat 1716.447 kb on + strandat 1716.447 kb on + strandat 1716.450 kb on - strandat 1716.554 kb on - strandat 1716.810 kb on + strand, within ydhHat 1716.989 kb on - strand, within ydhH

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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1,715,267 - -1.5
1,715,267 - -2.5
1,716,201 - ydhA b1639 0.34 -0.8
1,716,224 + ydhA b1639 0.41 -0.3
1,716,226 + ydhA b1639 0.41 -1.5
1,716,226 + ydhA b1639 0.41 +1.4
1,716,370 - ydhA b1639 0.85 -0.3
1,716,375 + ydhA b1639 0.86 +0.6
1,716,375 + ydhA b1639 0.86 +0.7
1,716,447 + -0.5
1,716,447 + -0.4
1,716,447 + -1.0
1,716,450 - +1.1
1,716,554 - -1.7
1,716,810 + ydhH b1640 0.26 -0.1
1,716,989 - ydhH b1640 0.43 -0.5

Or see this region's nucleotide sequence