Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapC and sapB overlap by 14 nucleotides sapB and sapA overlap by 4 nucleotides sapA and ymjA are separated by 312 nucleotides ymjA and ycjJ are separated by 133 nucleotides
b1292: sapC - predicted antimicrobial peptide transporter subunit (NCBI), at 1,351,652 to 1,352,542
sapC
b1293: sapB - predicted antimicrobial peptide transporter subunit (NCBI), at 1,352,529 to 1,353,494
sapB
b1294: sapA - predicted antimicrobial peptide transporter subunit (NCBI), at 1,353,491 to 1,355,134
sapA
b1295: ymjA - hypothetical protein (NCBI), at 1,355,447 to 1,355,692
ymjA
b1296: ycjJ - putative amino acid/amine transport protein (VIMSS), at 1,355,826 to 1,357,211
ycjJ
Position (kb)
1353
1354
1355
1356 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1352.770 kb on - strand, within sapB at 1352.770 kb on - strand, within sapB at 1352.790 kb on + strand, within sapB at 1353.131 kb on + strand, within sapB at 1353.522 kb on + strand at 1353.566 kb on - strand at 1353.747 kb on + strand, within sapA at 1353.747 kb on + strand, within sapA at 1353.825 kb on + strand, within sapA at 1353.825 kb on + strand, within sapA at 1353.841 kb on + strand, within sapA at 1353.841 kb on + strand, within sapA at 1353.844 kb on - strand, within sapA at 1353.890 kb on - strand, within sapA at 1353.890 kb on - strand, within sapA at 1353.912 kb on - strand, within sapA at 1353.987 kb on + strand, within sapA at 1354.118 kb on - strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.136 kb on + strand, within sapA at 1354.320 kb on - strand, within sapA at 1354.330 kb on - strand, within sapA at 1354.427 kb on - strand, within sapA at 1354.486 kb on - strand, within sapA at 1354.486 kb on - strand, within sapA at 1354.640 kb on + strand, within sapA at 1354.789 kb on - strand, within sapA at 1354.915 kb on - strand, within sapA at 1354.915 kb on - strand, within sapA at 1354.998 kb on - strand at 1355.215 kb on + strand at 1355.225 kb on - strand at 1355.225 kb on - strand at 1355.370 kb on - strand at 1355.370 kb on - strand at 1355.445 kb on - strand at 1355.485 kb on + strand, within ymjA at 1355.488 kb on - strand, within ymjA at 1355.488 kb on - strand, within ymjA at 1355.507 kb on - strand, within ymjA at 1355.713 kb on - strand at 1355.723 kb on - strand at 1355.726 kb on - strand at 1355.728 kb on - strand at 1355.853 kb on - strand at 1355.862 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 1,352,770 - sapB b1293 0.25 +1.6 1,352,770 - sapB b1293 0.25 +1.8 1,352,790 + sapB b1293 0.27 +3.0 1,353,131 + sapB b1293 0.62 +2.1 1,353,522 + +0.8 1,353,566 - -0.9 1,353,747 + sapA b1294 0.16 +1.3 1,353,747 + sapA b1294 0.16 +1.5 1,353,825 + sapA b1294 0.20 +1.8 1,353,825 + sapA b1294 0.20 -1.6 1,353,841 + sapA b1294 0.21 +2.7 1,353,841 + sapA b1294 0.21 +0.8 1,353,844 - sapA b1294 0.21 +1.3 1,353,890 - sapA b1294 0.24 -1.8 1,353,890 - sapA b1294 0.24 +1.0 1,353,912 - sapA b1294 0.26 +2.3 1,353,987 + sapA b1294 0.30 +2.2 1,354,118 - sapA b1294 0.38 +0.1 1,354,136 + sapA b1294 0.39 +1.5 1,354,136 + sapA b1294 0.39 +1.1 1,354,136 + sapA b1294 0.39 +0.8 1,354,136 + sapA b1294 0.39 +3.6 1,354,320 - sapA b1294 0.50 +0.8 1,354,330 - sapA b1294 0.51 +1.0 1,354,427 - sapA b1294 0.57 +0.4 1,354,486 - sapA b1294 0.61 -0.3 1,354,486 - sapA b1294 0.61 +1.3 1,354,640 + sapA b1294 0.70 +1.6 1,354,789 - sapA b1294 0.79 +1.1 1,354,915 - sapA b1294 0.87 +0.2 1,354,915 - sapA b1294 0.87 -0.4 1,354,998 - +2.0 1,355,215 + +0.2 1,355,225 - +1.5 1,355,225 - -1.9 1,355,370 - -0.2 1,355,370 - -0.8 1,355,445 - -0.6 1,355,485 + ymjA b1295 0.15 +0.2 1,355,488 - ymjA b1295 0.17 -0.8 1,355,488 - ymjA b1295 0.17 -0.9 1,355,507 - ymjA b1295 0.24 -1.0 1,355,713 - +2.1 1,355,723 - +0.5 1,355,726 - -0.4 1,355,728 - -1.2 1,355,853 - -0.3 1,355,862 + -2.0
Or see this region's nucleotide sequence