Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yccA and serT are separated by 206 nucleotides serT and hyaA are separated by 426 nucleotides hyaA and hyaB overlap by 4 nucleotides
b0970: yccA - inner membrane protein (NCBI), at 1,029,982 to 1,030,641
yccA
b0971: serT - tRNA-Ser (NCBI), at 1,030,848 to 1,030,935
serT
b0972: hyaA - hydrogenase 1, small subunit (NCBI), at 1,031,362 to 1,032,480
hyaA
b0973: hyaB - hydrogenase 1, large subunit (NCBI), at 1,032,477 to 1,034,270
hyaB
Position (kb)
1031
1032
1033 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1030.556 kb on - strand, within yccA at 1030.592 kb on - strand at 1030.592 kb on - strand at 1030.597 kb on + strand at 1030.613 kb on - strand at 1030.700 kb on - strand at 1030.756 kb on + strand at 1030.830 kb on - strand at 1030.955 kb on - strand at 1031.232 kb on + strand at 1031.317 kb on - strand at 1031.352 kb on + strand at 1031.352 kb on + strand at 1031.352 kb on - strand at 1031.352 kb on - strand at 1031.405 kb on + strand at 1031.438 kb on - strand at 1031.458 kb on + strand at 1031.478 kb on + strand, within hyaA at 1031.511 kb on + strand, within hyaA at 1031.552 kb on + strand, within hyaA at 1031.702 kb on - strand, within hyaA at 1031.702 kb on - strand, within hyaA at 1031.853 kb on - strand, within hyaA at 1031.853 kb on - strand, within hyaA at 1032.036 kb on + strand, within hyaA at 1032.165 kb on + strand, within hyaA at 1032.232 kb on + strand, within hyaA at 1032.241 kb on + strand, within hyaA at 1032.283 kb on + strand, within hyaA at 1032.283 kb on + strand, within hyaA at 1032.409 kb on - strand at 1032.409 kb on - strand at 1032.419 kb on + strand at 1032.419 kb on + strand at 1032.474 kb on + strand at 1032.474 kb on + strand at 1032.527 kb on + strand at 1032.527 kb on + strand at 1032.721 kb on + strand, within hyaB at 1032.822 kb on - strand, within hyaB at 1032.822 kb on - strand, within hyaB at 1032.884 kb on - strand, within hyaB at 1032.940 kb on - strand, within hyaB at 1032.972 kb on - strand, within hyaB at 1033.010 kb on + strand, within hyaB at 1033.010 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.093 kb on + strand, within hyaB at 1033.187 kb on + strand, within hyaB at 1033.214 kb on - strand, within hyaB at 1033.249 kb on + strand, within hyaB at 1033.249 kb on + strand, within hyaB at 1033.268 kb on + strand, within hyaB at 1033.268 kb on + strand, within hyaB at 1033.392 kb on - strand, within hyaB at 1033.419 kb on + strand, within hyaB at 1033.419 kb on + strand, within hyaB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 1,030,556 - yccA b0970 0.87 +0.1 1,030,592 - +0.9 1,030,592 - +1.6 1,030,597 + +0.7 1,030,613 - -0.5 1,030,700 - +0.5 1,030,756 + +0.2 1,030,830 - -2.0 1,030,955 - +1.4 1,031,232 + +3.4 1,031,317 - -1.9 1,031,352 + -1.0 1,031,352 + +0.9 1,031,352 - +0.7 1,031,352 - -0.0 1,031,405 + -2.0 1,031,438 - -0.1 1,031,458 + -0.6 1,031,478 + hyaA b0972 0.10 -2.0 1,031,511 + hyaA b0972 0.13 -1.0 1,031,552 + hyaA b0972 0.17 +0.3 1,031,702 - hyaA b0972 0.30 -0.8 1,031,702 - hyaA b0972 0.30 -1.2 1,031,853 - hyaA b0972 0.44 -0.7 1,031,853 - hyaA b0972 0.44 -0.8 1,032,036 + hyaA b0972 0.60 -1.5 1,032,165 + hyaA b0972 0.72 +1.0 1,032,232 + hyaA b0972 0.78 +0.1 1,032,241 + hyaA b0972 0.79 -0.5 1,032,283 + hyaA b0972 0.82 -0.7 1,032,283 + hyaA b0972 0.82 -0.1 1,032,409 - +1.0 1,032,409 - -1.0 1,032,419 + +1.0 1,032,419 + +0.3 1,032,474 + +0.7 1,032,474 + -2.4 1,032,527 + +0.5 1,032,527 + -0.3 1,032,721 + hyaB b0973 0.14 -0.1 1,032,822 - hyaB b0973 0.19 +1.0 1,032,822 - hyaB b0973 0.19 -1.4 1,032,884 - hyaB b0973 0.23 +1.6 1,032,940 - hyaB b0973 0.26 -2.0 1,032,972 - hyaB b0973 0.28 +0.3 1,033,010 + hyaB b0973 0.30 -1.8 1,033,010 + hyaB b0973 0.30 -0.5 1,033,093 + hyaB b0973 0.34 +0.8 1,033,093 + hyaB b0973 0.34 -0.4 1,033,093 + hyaB b0973 0.34 -0.3 1,033,187 + hyaB b0973 0.40 +3.3 1,033,214 - hyaB b0973 0.41 -0.3 1,033,249 + hyaB b0973 0.43 -0.2 1,033,249 + hyaB b0973 0.43 -1.8 1,033,268 + hyaB b0973 0.44 +0.2 1,033,268 + hyaB b0973 0.44 -0.9 1,033,392 - hyaB b0973 0.51 +0.3 1,033,419 + hyaB b0973 0.53 -0.8 1,033,419 + hyaB b0973 0.53 +0.4
Or see this region's nucleotide sequence