Strain Fitness in Escherichia coli BW25113 around b0657

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsH-3 and lnt are separated by 329 nucleotideslnt and ybeX are separated by 24 nucleotidesybeX and ybeY are separated by 89 nucleotides b0656: insH-3 - IS5 transposase and trans-activator (NCBI), at 687,220 to 688,236 insH-3 b0657: lnt - apolipoprotein N-acyltransferase (NCBI), at 688,566 to 690,104 lnt b0658: ybeX - predicteed ion transport (NCBI), at 690,129 to 691,007 ybeX b0659: ybeY - hypothetical protein (NCBI), at 691,097 to 691,564 ybeY Position (kb) 688 689 690 691Strain fitness (log2 ratio) -1 0 1 2at 688.239 kb on + strandat 688.499 kb on + strandat 688.588 kb on + strandat 690.152 kb on - strandat 690.252 kb on + strand, within ybeXat 690.312 kb on + strand, within ybeXat 690.370 kb on + strand, within ybeXat 690.378 kb on - strand, within ybeXat 690.378 kb on - strand, within ybeXat 690.415 kb on + strand, within ybeXat 690.415 kb on + strand, within ybeXat 690.435 kb on - strand, within ybeXat 690.457 kb on - strand, within ybeXat 690.457 kb on - strand, within ybeXat 690.517 kb on + strand, within ybeXat 690.589 kb on - strand, within ybeXat 690.609 kb on - strand, within ybeXat 690.713 kb on - strand, within ybeXat 690.779 kb on - strand, within ybeXat 690.795 kb on - strand, within ybeXat 690.795 kb on - strand, within ybeXat 690.809 kb on + strand, within ybeXat 690.817 kb on - strand, within ybeXat 691.017 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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688,239 + +0.8
688,499 + -0.3
688,588 + -1.0
690,152 - +0.8
690,252 + ybeX b0658 0.14 +1.1
690,312 + ybeX b0658 0.21 +0.1
690,370 + ybeX b0658 0.27 +1.1
690,378 - ybeX b0658 0.28 +0.1
690,378 - ybeX b0658 0.28 +1.1
690,415 + ybeX b0658 0.33 +1.1
690,415 + ybeX b0658 0.33 +1.1
690,435 - ybeX b0658 0.35 +1.1
690,457 - ybeX b0658 0.37 +1.1
690,457 - ybeX b0658 0.37 +2.1
690,517 + ybeX b0658 0.44 +1.1
690,589 - ybeX b0658 0.52 +1.1
690,609 - ybeX b0658 0.55 +1.1
690,713 - ybeX b0658 0.66 +1.1
690,779 - ybeX b0658 0.74 +1.1
690,795 - ybeX b0658 0.76 +1.1
690,795 - ybeX b0658 0.76 +1.1
690,809 + ybeX b0658 0.77 +0.1
690,817 - ybeX b0658 0.78 +1.1
691,017 - +0.7

Or see this region's nucleotide sequence