Strain Fitness in Escherichia coli BW25113 around b0601

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybdL and ybdM are separated by 0 nucleotidesybdM and ybdN overlap by 28 nucleotides b0600: ybdL - putative aminotransferase (NCBI), at 632,809 to 633,969 ybdL b0601: ybdM - hypothetical protein (NCBI), at 633,970 to 634,599 ybdM b0602: ybdN - hypothetical protein (NCBI), at 634,572 to 635,792 ybdN Position (kb) 633 634 635Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 633.223 kb on + strand, within ybdLat 633.228 kb on + strand, within ybdLat 633.228 kb on + strand, within ybdLat 633.228 kb on - strand, within ybdLat 633.320 kb on - strand, within ybdLat 633.322 kb on - strand, within ybdLat 633.327 kb on - strand, within ybdLat 633.351 kb on - strand, within ybdLat 633.418 kb on + strand, within ybdLat 633.418 kb on - strand, within ybdLat 633.418 kb on - strand, within ybdLat 633.442 kb on - strand, within ybdLat 633.442 kb on - strand, within ybdLat 633.541 kb on - strand, within ybdLat 633.551 kb on - strand, within ybdLat 633.570 kb on - strand, within ybdLat 633.572 kb on + strand, within ybdLat 633.587 kb on + strand, within ybdLat 633.597 kb on - strand, within ybdLat 633.717 kb on - strand, within ybdLat 633.825 kb on + strand, within ybdLat 633.853 kb on - strand, within ybdLat 633.970 kb on + strandat 634.194 kb on + strand, within ybdMat 634.206 kb on + strand, within ybdMat 634.214 kb on - strand, within ybdMat 634.214 kb on - strand, within ybdMat 634.214 kb on - strand, within ybdMat 634.214 kb on - strand, within ybdMat 634.214 kb on - strand, within ybdMat 634.309 kb on + strand, within ybdMat 634.335 kb on - strand, within ybdMat 634.335 kb on - strand, within ybdMat 634.337 kb on - strand, within ybdMat 634.464 kb on - strand, within ybdMat 634.477 kb on - strand, within ybdMat 634.486 kb on - strand, within ybdMat 634.486 kb on - strand, within ybdMat 634.593 kb on + strandat 634.672 kb on + strandat 634.687 kb on + strandat 634.720 kb on - strand, within ybdNat 634.963 kb on + strand, within ybdNat 634.977 kb on + strand, within ybdNat 635.018 kb on + strand, within ybdNat 635.018 kb on + strand, within ybdNat 635.039 kb on - strand, within ybdNat 635.132 kb on + strand, within ybdNat 635.140 kb on - strand, within ybdNat 635.140 kb on - strand, within ybdNat 635.298 kb on - strand, within ybdNat 635.431 kb on - strand, within ybdNat 635.437 kb on - strand, within ybdNat 635.457 kb on - strand, within ybdNat 635.475 kb on + strand, within ybdNat 635.500 kb on + strand, within ybdNat 635.505 kb on + strand, within ybdNat 635.505 kb on + strand, within ybdNat 635.542 kb on + strand, within ybdNat 635.545 kb on - strand, within ybdNat 635.545 kb on - strand, within ybdN

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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633,223 + ybdL b0600 0.36 -0.7
633,228 + ybdL b0600 0.36 -1.9
633,228 + ybdL b0600 0.36 -0.9
633,228 - ybdL b0600 0.36 +0.9
633,320 - ybdL b0600 0.44 -0.6
633,322 - ybdL b0600 0.44 +0.1
633,327 - ybdL b0600 0.45 -1.8
633,351 - ybdL b0600 0.47 -0.1
633,418 + ybdL b0600 0.52 +1.1
633,418 - ybdL b0600 0.52 +0.3
633,418 - ybdL b0600 0.52 -3.2
633,442 - ybdL b0600 0.55 -0.4
633,442 - ybdL b0600 0.55 +0.4
633,541 - ybdL b0600 0.63 -0.1
633,551 - ybdL b0600 0.64 +1.6
633,570 - ybdL b0600 0.66 +0.2
633,572 + ybdL b0600 0.66 -0.1
633,587 + ybdL b0600 0.67 -0.4
633,597 - ybdL b0600 0.68 -0.5
633,717 - ybdL b0600 0.78 +1.5
633,825 + ybdL b0600 0.88 +1.0
633,853 - ybdL b0600 0.90 +1.1
633,970 + -0.5
634,194 + ybdM b0601 0.36 +0.3
634,206 + ybdM b0601 0.37 +1.2
634,214 - ybdM b0601 0.39 +1.3
634,214 - ybdM b0601 0.39 +0.1
634,214 - ybdM b0601 0.39 +2.8
634,214 - ybdM b0601 0.39 +0.5
634,214 - ybdM b0601 0.39 -0.2
634,309 + ybdM b0601 0.54 -1.2
634,335 - ybdM b0601 0.58 -0.7
634,335 - ybdM b0601 0.58 -2.2
634,337 - ybdM b0601 0.58 -0.1
634,464 - ybdM b0601 0.78 -2.4
634,477 - ybdM b0601 0.80 +0.1
634,486 - ybdM b0601 0.82 -0.1
634,486 - ybdM b0601 0.82 -1.2
634,593 + -0.4
634,672 + -0.5
634,687 + +0.3
634,720 - ybdN b0602 0.12 -0.3
634,963 + ybdN b0602 0.32 +0.7
634,977 + ybdN b0602 0.33 +1.3
635,018 + ybdN b0602 0.37 -2.5
635,018 + ybdN b0602 0.37 -0.9
635,039 - ybdN b0602 0.38 +0.9
635,132 + ybdN b0602 0.46 +0.6
635,140 - ybdN b0602 0.47 +0.9
635,140 - ybdN b0602 0.47 -1.0
635,298 - ybdN b0602 0.59 +0.2
635,431 - ybdN b0602 0.70 +1.6
635,437 - ybdN b0602 0.71 +0.2
635,457 - ybdN b0602 0.72 +2.1
635,475 + ybdN b0602 0.74 -1.8
635,500 + ybdN b0602 0.76 +1.7
635,505 + ybdN b0602 0.76 -0.5
635,505 + ybdN b0602 0.76 +2.1
635,542 + ybdN b0602 0.79 +0.1
635,545 - ybdN b0602 0.80 -1.2
635,545 - ybdN b0602 0.80 +0.5

Or see this region's nucleotide sequence