Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt secF and yajD are separated by 128 nucleotides yajD and tsx are separated by 176 nucleotides tsx and yajI are separated by 298 nucleotides yajI and ybaD are separated by 150 nucleotides
b0409: secF - protein export protein SecF (NCBI), at 428,729 to 429,700
secF
b0410: yajD - hypothetical protein (NCBI), at 429,829 to 430,176
yajD
b0411: tsx - nucleoside channel, receptor of phage T6 and colicin K (NCBI), at 430,353 to 431,237
tsx
b0412: yajI - orf, hypothetical protein (VIMSS), at 431,536 to 432,075
yajI
b0413: ybaD - hypothetical protein (NCBI), at 432,226 to 432,675
ybaD
Position (kb) 430
431
432 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 429.625 kb on + strand at 429.625 kb on + strand at 429.757 kb on + strand at 429.757 kb on + strand at 429.917 kb on + strand, within yajD at 429.944 kb on + strand, within yajD at 429.958 kb on + strand, within yajD at 429.988 kb on + strand, within yajD at 430.066 kb on + strand, within yajD at 430.079 kb on - strand, within yajD at 430.079 kb on - strand, within yajD at 430.090 kb on - strand, within yajD at 430.300 kb on + strand at 430.349 kb on - strand at 430.382 kb on - strand at 430.430 kb on + strand at 430.453 kb on + strand, within tsx at 430.471 kb on - strand, within tsx at 430.471 kb on - strand, within tsx at 430.473 kb on + strand, within tsx at 430.473 kb on + strand, within tsx at 430.483 kb on + strand, within tsx at 430.483 kb on + strand, within tsx at 430.491 kb on - strand, within tsx at 430.506 kb on - strand, within tsx at 430.568 kb on - strand, within tsx at 430.624 kb on - strand at 430.709 kb on + strand, within tsx at 430.709 kb on + strand, within tsx at 430.753 kb on + strand, within tsx at 430.759 kb on + strand, within tsx at 430.759 kb on + strand, within tsx at 430.777 kb on - strand, within tsx at 430.779 kb on + strand, within tsx at 430.779 kb on + strand, within tsx at 430.796 kb on - strand, within tsx at 430.803 kb on - strand, within tsx at 430.803 kb on - strand, within tsx at 430.822 kb on + strand, within tsx at 430.823 kb on + strand, within tsx at 430.847 kb on - strand, within tsx at 430.863 kb on + strand, within tsx at 430.869 kb on - strand, within tsx at 430.869 kb on - strand, within tsx at 430.892 kb on + strand, within tsx at 430.905 kb on - strand, within tsx at 430.906 kb on + strand, within tsx at 431.038 kb on + strand, within tsx at 431.062 kb on + strand, within tsx at 431.212 kb on - strand at 431.212 kb on - strand at 431.311 kb on - strand at 431.365 kb on - strand at 431.365 kb on - strand at 431.541 kb on + strand at 431.565 kb on - strand at 431.709 kb on - strand, within yajI at 431.770 kb on - strand, within yajI at 431.775 kb on + strand, within yajI at 431.783 kb on - strand, within yajI at 431.783 kb on - strand, within yajI at 431.845 kb on + strand, within yajI at 431.901 kb on - strand, within yajI at 431.901 kb on - strand, within yajI at 431.973 kb on + strand, within yajI at 431.973 kb on + strand, within yajI at 432.007 kb on + strand, within yajI at 432.007 kb on + strand, within yajI at 432.017 kb on - strand, within yajI at 432.056 kb on - strand at 432.071 kb on + strand at 432.071 kb on + strand at 432.078 kb on - strand at 432.105 kb on + strand at 432.105 kb on + strand at 432.115 kb on + strand at 432.207 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 429,625 + -0.0 429,625 + -1.6 429,757 + -2.8 429,757 + -0.4 429,917 + yajD b0410 0.25 +0.2 429,944 + yajD b0410 0.33 -0.3 429,958 + yajD b0410 0.37 +0.1 429,988 + yajD b0410 0.46 +1.9 430,066 + yajD b0410 0.68 -1.8 430,079 - yajD b0410 0.72 -1.7 430,079 - yajD b0410 0.72 -1.5 430,090 - yajD b0410 0.75 -0.1 430,300 + +0.0 430,349 - -1.2 430,382 - -2.3 430,430 + -1.5 430,453 + tsx b0411 0.11 -1.5 430,471 - tsx b0411 0.13 -0.2 430,471 - tsx b0411 0.13 -1.6 430,473 + tsx b0411 0.14 +0.1 430,473 + tsx b0411 0.14 -0.2 430,483 + tsx b0411 0.15 -0.5 430,483 + tsx b0411 0.15 +3.4 430,491 - tsx b0411 0.16 -0.3 430,506 - tsx b0411 0.17 +0.1 430,568 - tsx b0411 0.24 -0.2 430,624 - -0.9 430,709 + tsx b0411 0.40 -2.5 430,709 + tsx b0411 0.40 -2.2 430,753 + tsx b0411 0.45 -0.8 430,759 + tsx b0411 0.46 -0.5 430,759 + tsx b0411 0.46 -3.0 430,777 - tsx b0411 0.48 -1.8 430,779 + tsx b0411 0.48 -2.4 430,779 + tsx b0411 0.48 -3.0 430,796 - tsx b0411 0.50 -1.9 430,803 - tsx b0411 0.51 -2.5 430,803 - tsx b0411 0.51 +0.0 430,822 + tsx b0411 0.53 -1.7 430,823 + tsx b0411 0.53 -1.0 430,847 - tsx b0411 0.56 -0.8 430,863 + tsx b0411 0.58 -3.3 430,869 - tsx b0411 0.58 +0.1 430,869 - tsx b0411 0.58 -0.5 430,892 + tsx b0411 0.61 -2.7 430,905 - tsx b0411 0.62 +0.1 430,906 + tsx b0411 0.62 -2.3 431,038 + tsx b0411 0.77 -2.3 431,062 + tsx b0411 0.80 +1.0 431,212 - -3.7 431,212 - -1.7 431,311 - -1.6 431,365 - +0.7 431,365 - +0.3 431,541 + +1.0 431,565 - -1.7 431,709 - yajI b0412 0.32 +0.2 431,770 - yajI b0412 0.43 +1.1 431,775 + yajI b0412 0.44 -0.3 431,783 - yajI b0412 0.46 +0.4 431,783 - yajI b0412 0.46 -2.8 431,845 + yajI b0412 0.57 -0.0 431,901 - yajI b0412 0.68 -0.1 431,901 - yajI b0412 0.68 -0.3 431,973 + yajI b0412 0.81 +1.5 431,973 + yajI b0412 0.81 +0.5 432,007 + yajI b0412 0.87 -1.0 432,007 + yajI b0412 0.87 -1.2 432,017 - yajI b0412 0.89 -3.7 432,056 - +0.6 432,071 + +0.8 432,071 + -0.5 432,078 - +1.4 432,105 + +0.7 432,105 + -0.6 432,115 + +1.4 432,207 - +1.1
Or see this region's nucleotide sequence