Strain Fitness in Escherichia coli BW25113 around b4490

Experiment: LB Anaerobic with Trimethoprim 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntputP and efeU are separated by 542 nucleotidesefeU and ycdO are separated by 57 nucleotides b1015: putP - proline:sodium symporter (NCBI), at 1,078,528 to 1,080,036 putP b4490: efeU - no description, at 1,080,579 to 1,081,408 efeU b1018: ycdO - hypothetical protein (NCBI), at 1,081,466 to 1,082,593 ycdO Position (kb) 1080 1081 1082Strain fitness (log2 ratio) -1 0 1 2at 1079.650 kb on + strand, within putPat 1079.750 kb on - strand, within putPat 1079.750 kb on - strand, within putPat 1079.770 kb on + strand, within putPat 1079.770 kb on + strand, within putPat 1079.773 kb on - strand, within putPat 1079.783 kb on - strand, within putPat 1079.873 kb on + strand, within putPat 1079.881 kb on - strand, within putPat 1079.940 kb on + strandat 1079.940 kb on + strandat 1079.943 kb on + strandat 1079.943 kb on + strandat 1080.086 kb on + strandat 1080.086 kb on + strandat 1080.094 kb on - strandat 1080.094 kb on - strandat 1080.229 kb on - strandat 1080.595 kb on + strandat 1080.684 kb on - strand, within efeUat 1080.696 kb on - strand, within efeUat 1080.701 kb on - strand, within efeUat 1080.787 kb on + strand, within efeUat 1080.823 kb on - strand, within efeUat 1080.823 kb on - strand, within efeUat 1080.898 kb on - strand, within efeUat 1080.907 kb on + strand, within efeUat 1080.924 kb on + strand, within efeUat 1080.924 kb on + strand, within efeUat 1080.957 kb on + strand, within efeUat 1081.013 kb on + strand, within efeUat 1081.047 kb on + strand, within efeUat 1081.081 kb on + strand, within efeUat 1081.246 kb on - strand, within efeUat 1081.246 kb on - strand, within efeUat 1081.251 kb on - strand, within efeUat 1081.269 kb on - strand, within efeUat 1081.269 kb on - strand, within efeUat 1081.377 kb on - strandat 1081.542 kb on - strandat 1081.577 kb on + strandat 1081.663 kb on + strand, within ycdOat 1081.663 kb on + strand, within ycdOat 1081.663 kb on - strand, within ycdOat 1081.663 kb on - strand, within ycdOat 1081.678 kb on - strand, within ycdOat 1081.852 kb on + strand, within ycdOat 1081.852 kb on + strand, within ycdOat 1081.908 kb on + strand, within ycdOat 1081.944 kb on + strand, within ycdOat 1081.979 kb on + strand, within ycdOat 1082.039 kb on + strand, within ycdOat 1082.039 kb on + strand, within ycdOat 1082.156 kb on - strand, within ycdOat 1082.179 kb on + strand, within ycdOat 1082.192 kb on - strand, within ycdOat 1082.382 kb on + strand, within ycdOat 1082.392 kb on + strand, within ycdO

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Trimethoprim 0.0000003125 mM
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1,079,650 + putP b1015 0.74 +0.3
1,079,750 - putP b1015 0.81 -0.9
1,079,750 - putP b1015 0.81 -0.1
1,079,770 + putP b1015 0.82 +0.3
1,079,770 + putP b1015 0.82 -0.3
1,079,773 - putP b1015 0.83 -0.8
1,079,783 - putP b1015 0.83 -0.3
1,079,873 + putP b1015 0.89 -1.2
1,079,881 - putP b1015 0.90 -0.5
1,079,940 + +1.1
1,079,940 + +0.7
1,079,943 + -0.0
1,079,943 + +0.9
1,080,086 + +0.3
1,080,086 + +0.4
1,080,094 - +1.8
1,080,094 - +0.7
1,080,229 - -0.2
1,080,595 + +0.2
1,080,684 - efeU b4490 0.13 -0.1
1,080,696 - efeU b4490 0.14 +1.0
1,080,701 - efeU b4490 0.15 +0.9
1,080,787 + efeU b4490 0.25 +0.0
1,080,823 - efeU b4490 0.29 +0.1
1,080,823 - efeU b4490 0.29 +0.7
1,080,898 - efeU b4490 0.38 -0.5
1,080,907 + efeU b4490 0.40 +0.2
1,080,924 + efeU b4490 0.42 +1.1
1,080,924 + efeU b4490 0.42 +0.2
1,080,957 + efeU b4490 0.46 +0.2
1,081,013 + efeU b4490 0.52 +0.7
1,081,047 + efeU b4490 0.56 -0.1
1,081,081 + efeU b4490 0.60 +1.1
1,081,246 - efeU b4490 0.80 +0.8
1,081,246 - efeU b4490 0.80 -0.2
1,081,251 - efeU b4490 0.81 +0.2
1,081,269 - efeU b4490 0.83 +0.2
1,081,269 - efeU b4490 0.83 +1.7
1,081,377 - -0.1
1,081,542 - -0.5
1,081,577 + -0.4
1,081,663 + ycdO b1018 0.17 -0.7
1,081,663 + ycdO b1018 0.17 +0.2
1,081,663 - ycdO b1018 0.17 +0.2
1,081,663 - ycdO b1018 0.17 +0.7
1,081,678 - ycdO b1018 0.19 -0.1
1,081,852 + ycdO b1018 0.34 +1.3
1,081,852 + ycdO b1018 0.34 -0.1
1,081,908 + ycdO b1018 0.39 +1.1
1,081,944 + ycdO b1018 0.42 +0.1
1,081,979 + ycdO b1018 0.45 +0.7
1,082,039 + ycdO b1018 0.51 -0.2
1,082,039 + ycdO b1018 0.51 +1.2
1,082,156 - ycdO b1018 0.61 +0.3
1,082,179 + ycdO b1018 0.63 +0.2
1,082,192 - ycdO b1018 0.64 -0.8
1,082,382 + ycdO b1018 0.81 -0.5
1,082,392 + ycdO b1018 0.82 +1.5

Or see this region's nucleotide sequence